Effects of Salicylate and Glucose on Biodegradation of Phenanthrene by Burkholderia cepacia PM07

  • LEE DAE SUNG (Department of Chemical Engineering, School of Environmental Science and Engineering, Pohang University of Science and Technology) ;
  • LEE MIN WOO (Department of Chemical Engineering, School of Environmental Science and Engineering, Pohang University of Science and Technology) ;
  • WOO SEUNG HAN (Department of Chemical Engineering, Hanbat National University) ;
  • PARK JONG MOON (Department of Chemical Engineering, School of Environmental Science and Engineering, Pohang University of Science and Technology)
  • Published : 2005.08.01


The stimulatory effects of exogenous salicylate as a pathway inducer on phenanthrene biodegradation were investigated using Burkholderia cepacia PM07. The phenanthrene degradation rate was greatly enhanced by increasing the salicylate additions, and the maximum rate was 19.6 mg $I^{-1}\;d^{-1}$ with the addition of 200 mg $I^{-1}$ of salicylate, 3.5 times higher than that (5.6 mg $I^{-1}\;d^{-1}$) without the addition of salicylate. The degradation rate was decreased at higher concentrations of salicylate (above 500 mg$I^{-1}$), and cell growth was significantly inhibited. The phenanthrene degradation was not affected by increasing glucose up to 2 g $I^{-1}$, although dramatic microbial growth was obtained. The stimulatory effect of exogenous salicylate decreased in the presence of glucose. After the addition of 200 mg $I^{-1}$ of salicylate, approximately $60\%$ of the initial phenanthrene (50 mg $I^{-1}$) was degraded after 96 h. However, with extra addition of 200 mg $I^{-1}$ of glucose, the phenanthrene degradation rate decreased, and only $18.5\%$ of the initial phenanthrene was degraded.


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