Expression of a Cu-Zn Superoxide Dismutase Gene in Response to Stresses and Phytohormones in Rehmannia Glutinosa

  • Park, Myoung-Ryoul (Division of Biological Resources Sciences, Chonbuk Nat'l Univ.) ;
  • Ryu, Sang-Soo (Division of Biological Resources Sciences, Chonbuk Nat'l Univ.) ;
  • Yoo, Nam-Hee (Institute of Agricultural Science and Technology, Chonbuk Nat'l Univ.) ;
  • Yu, Chang-Yeon (Division of Applied Plant Sciences, Kangwon Nat'l Univ.) ;
  • Yun, Song-Joong (Division of Biological Resources Sciences, Chonbuk Nat'l Univ.,Institute of Agricultural Science and Technology, Chonbuk Nat'l Univ.)
  • Published : 2005.10.30

Abstract

Superoxide dismutases (SOD) are metalloenzymes that convert $O_2^-\;to\;H_2O_2$. Rehmannia glutinosa is highly tolerant to paraquat-induced oxidative stress. The primary objective of this study was to characterize regulation of SOD gene expression in R. glutinosa in response to oxidative stresses and hormones. A full-length putative SOD clone (RgCu-ZnSOD1) was isolated from the leaf cDNA library of R. glutinosa using an expressed sequence tag clone as a probe. RgCu-ZnSOD1 cDNA is 777 bp in length and contains an open reading frame for a polypeptide consisted of 152 amino acid residues. The deduced amino acid sequence of the clone shows highest sequence similarity to the cytosolic Cu-ZnSODs. The two to three major bands with several minor ones on the Southern blots indicate that RgCu-ZnSOD1 is a member of a small multi-gene family. RgCuZnSOD1 mRNA was constitutively expressed in the leaf, flower and root. The expression of RgCu-ZnSOD1 mRNA was increased about 20% by wounding and paraquat, but decreased over 50% by ethylene and $GA_3$. This result indicates that the RgCu-ZnSOD1 expression is regulated differentially by different stresses and phytohormones at the transcription level. The RgCu-ZnSOD1 sequence and information on its regulation will be useful in investigating the role of SOD in the paraquat tolerance of R. glutinosa.

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