Protective Effect of N-Acetylcysteine on Progression of Adriamycin-induced Nephyopathy

  • Han, Sang-Woong (Department of Internal Medicine, Hanyang University College of Medicine) ;
  • Kim, Ho-Jung (Department of Internal Medicine, Hanyang University College of Medicine) ;
  • Paik, Seung-Sam (Department of Pathology, Hanyang University College of Medicine) ;
  • Lee, Jong-Un (Department of Physiology, Chonnam National University Medical School)
  • Published : 2005.06.21

Abstract

Effects of antioxidants on the established nephropathy were investigated. The experimental nephropathy was induced in rats by intravenous injection of adriamycin (2 mg/kg). Six weeks later, when proteinuria was apparent, the rats were supplemented with N-acetylcysteine (NAC, 1 g/kg/day) in drinking water for additional 6 weeks. Glomerulosclerosis score and tubulointerstitial injury index were determined by light microscopy. Expression of transforming growth factor (TGF) ${\beta}1$ and laminin ${\beta}1$ was determined in the renal cortex by reverse transcription-polymerase chain reaction, Western blotting, immunohistochemistry, and immunogold electron microscopy. The adriamycin-induced proteinuria as well as the glomerulosclerosis and tubulointerstitial injury was ameliorated by the treatment with NAC. Adriamycin increased the expression of TGF ${\beta}1$ mRNA and protein, which was ameliorated by NAC. Although the expression of laminin ${\beta}1$ mRNA was increased, adriamycin did not significantly alter that of its protein. These results indicate that antioxidants ameliorate the established nephropathy in association with normalization of overexpressed TGF ${\beta}1$.

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