Effects of Sphingosine-1-phosphate, Furosemide and Indomethacin on Mucin Release from Airway Goblet Cells

  • Kim, Ju-Ock (Pulmonology Section, Department of Internal Medicine, Chungnam National University Hospital) ;
  • Kim, Hyung-Soo (Department of Pharmacology, College of Medicine, Chungnam National University) ;
  • Kim, Sun-Young (Pulmonology Section, Department of Internal Medicine, Chungnam National University Hospital) ;
  • Jeong, Seong-Soo (Pulmonology Section, Department of Internal Medicine, Chungnam National University Hospital) ;
  • Heo, Ho-Jin (Department of Pharmacology, College of Medicine, Chungnam National University) ;
  • Seok, Jeong-Ho (Department of Pharmacology, College of Medicine, Chungnam National University) ;
  • Lee, Choong-Jae (Department of Pharmacology, College of Medicine, Chungnam National University)
  • Published : 2005.06.21

Abstract

In this study, we investigated whether sphingosine-1-phosphate, furosemide, and indomethacin affect mucin release from airway goblet cells. Confluent primary hamster tracheal surface epithelial cells were metabolically radiolabeled and chased for 30 min or 24 hr in the presence of varying concentrations of the above agents to assess the effects on $^3H-mucin$ release. Sphingosine-1-phosphate stimulated mucin release during 30 min of treatment period in a dose-dependent manner. However, furosemide and indomethacin showed no effect on both basal and stimulated mucin release during 30 min or 24 hr of treatment period. We conclude that sphingosine-1-phosphate can affect mucin release by directly acting on airway mucin-secreting cells.

References

  1. Cummings RJ, Parinandi NL, Zaiman A, Wang L, Usatyuk PV, Garcia JG, Natarajan V. Phospholipase D activation by sphingosine- 1-phosphate regulates interleukin-8 secretion in human bronchial epithelial cells. J Biol Chem 277: 30227-30235, 2002 https://doi.org/10.1074/jbc.M111078200
  2. Kim KC, Rearick JI, Nettesheim P, Jetten AM. Biochemical characterization of mucous glycoproteins synthesized and secreted by hamster tracheal epithelial cells in primary culture. J Biol Chem 260: 4021-4027, 1985
  3. Ko KH, Lee CJ, Shin CY, Jo M, Kim KC. Inhibition of mucin release from airway goblet cells by polycationic peptides. Am J Physiol 277: L811-L815, 1999
  4. Tamaoki J, Chiyotani A, Kobayashi K, Sakai N, Kanemura T, Takizawa T. Effect of indomethacin on bronchorrhea in patients with chronic bronchitis, diffuse panbroncjiolitis, or bronchiectasis. Am Rev Respir Dis 145: 548-552, 1992 https://doi.org/10.1164/ajrccm/145.3.548
  5. Kim KC, McCracken K, Lee BC, Shin CY, Jo MJ, Lee CJ, Ko KH. Airway goblet cell mucin: its structure and regulation of secretion. Eur Respir J 10: 2644-2649, 1997 https://doi.org/10.1183/09031936.97.10112644
  6. Prandota J. Clinical pharmacology of furosemide in children: a supplement. Am J Ther 8: 275-289, 2001 https://doi.org/10.1097/00045391-200107000-00010
  7. Wasano K, Kim KC, Niles RM, Brody JS. Membrane differentiation markers of airway epithelial secretory cells. J Histochem Cytochem 36: 167-178, 1988 https://doi.org/10.1177/36.2.3335774
  8. Rabano M, Pena A, Brizuela A, Marino A, Macarulla JM, Trueba M, Gomez-Munoz A. Sphingosine-1-phosphate stimulates cortisol secretion. FEBS Lett 535: 101-105, 2003 https://doi.org/10.1016/S0014-5793(02)03882-6
  9. Ammit AJ, Hastie AT, Edsall LC, Hoffman RK, Amrani Y, Krymska VP. Sphingosine-1-phosphate modulates human airway smooth muscle cell functions that promote inflammation and airway remodeling in asthma. FASEB J 15: 1212-1214, 2001 https://doi.org/10.1096/fj.00-0742fje
  10. Bianco S, Pieroni MG, Refini RM, Rottoli L, Sestini P. Protective effect of inhaled furosemide on allergen-induced early and late asthmatic reactions. N Engl J Med 321: 1069-1073, 1989 https://doi.org/10.1056/NEJM198910193211602
  11. Jeffery PK. Anti-inflammatory drugs and experimental bronchitis. Eur J Respir Dis 146: 245-257, 1986
  12. Jolly PS, Rosenfeldt HM, Milstien S, Spiegel S. The roles of sphingosine-1-phosphate in asthma. Mol Immunol 38: 1239- 1245, 2001 https://doi.org/10.1016/S0161-5890(02)00070-6
  13. Kim KC, Wasano K, Niles RM, Schuster JE, Stone PJ, Brody JS. Human neutrophil elastase releases cell surface mucins from primary cultures of hamster tracheal epithelial cells. Proc Natl Acad Sci USA 84: 9304-9308, 1987 https://doi.org/10.1073/pnas.84.24.9304
  14. Mutschler E, Derendorf H. Drug actions. Boca Raton, Florida, CRC Press, p 410-411, 1995
  15. Lee CJ, Paik SH, Ko KH, Kim KC. Effects of polycationic peptides on mucin release from airway goblet cells: relationship between polymer size and activity. Inflamm Res 51: 490-494, 2002 https://doi.org/10.1007/PL00012417
  16. Ellis EF. Asthma in childhood. J Allergy Clin Immunol 72 (suppl): 526-539, 1985