Effects of a ${\delta}-opioid$ Agonist on the Brainstem Vestibular Nuclear Neuronal Activity of Rats

  • Kim, Tae-Sun (Department of Neurosurgery, Chonnam National University Medicine School) ;
  • Huang, Mei (Department of Physiology, Chonnam National University Medicine School) ;
  • Jang, Myung-Joo (Department of Physiology, Chonnam National University Medicine School) ;
  • Jeong, Han-Seong (Department of Physiology, Chonnam National University Medicine School) ;
  • Park, Jong-Seong (Department of Physiology, Chonnam National University Medicine School)
  • 발행 : 2005.06.21

초록

This study was undertaken to investigate the effects of [$D-Ala^2$, D-Leu^5$]-enkephalin (DADLE) on the spontaneous activity of medial vestibular nuclear neurons of the rat. Sprague-Dawley rats, aged 14 to 16 days, were anesthetized with ether and decapitated. After enzymatic digestion, the brain stem portion of medial vestibular nuclear neuron was obtained by micropunching. The dissociated neurons were transferred to a recording chamber mounted on an inverted microscope, and spontaneous action potentials were recorded by standard patch-clamp techniques. The spontaneous action potentials were increased by DADLE in 12 cells and decreased in 3 cells. The spike frequency and resting membrane potential of these cells were increased by DADLE. The depth of afterhyperpolarization was not affected by DADLE. The potassium currents were decreased in 20 cells and increased in 5 cells. These results suggest that DADLE increases the neuronal activity of the medial vestibular nuclear neurons by altering resting membrane potential.

참고문헌

  1. Dewey WL, Brase D, Weleh SP. Pain control with opioid analgesics. In: Brody TM ed, Human Pharmacology: molecular to clinical. 2nd ed. Mosby, St. Louis, p 379-380, 1994
  2. Darlington CL, Smith PF. The recovery of static vestibular function following peripheral vestibular lesions in mammals: the intrinsic mechanism hypothesis. J Vestib Res 6: 185-201, 1996 https://doi.org/10.1016/0957-4271(95)02042-X
  3. Jeong HS, Lim YC, Kim TS, Heo T, Jung SM, Cho YB, Jun JY, Park JS. Excitatory effects of 5-hydroxytryptamine on the medial vestibular nuclear neuron via the 5-$HT_{2}$ receptor. Neuroreport 14: 2001-2004, 2003
  4. Saika T, Takeda N, Kiyama H, Kubo T, Tohyama M, Matsunaga T. Changes in preproenkephalin mRNA after unilateral and bilateral labyrinthectomy in the rat medial vesibular nucleus. Mol Brain Res 237-240, 1993
  5. Zanni M, Ciardino L, Toschi L, Galetti G, Calza L. Distribution of neurotransmission, neuropeptides and receptors in the vestibular nuclei complex of the rat: An immunocytochemical, in situ hybridzation and quantitaitve receptor autoradiographic study. Brain Res Bull 36: 443-452, 1995 https://doi.org/10.1016/0361-9230(94)00193-5
  6. Hamil OP, Marty A, Neher E, Sakman B, Sigworth F. Improved patch clamp techniques for high resolution current recording from cells and cell free membrane patches. Pflug Arch 391: 85- 100, 1981 https://doi.org/10.1007/BF00656997
  7. Lin Y, Carpenter DO. Direct excitatory opiate effects mediate by non-synaptic actions on rat medial vestibular neurons. Eur J Pharmacol 262: 99-106, 1994 https://doi.org/10.1016/0014-2999(94)90032-9
  8. Park JS, Jeong HS, Kim TS, Kim YS, Kim JH, Jang MJ, Kim JY, Huang M. Effects of 5-$HT_{1}$ receptor agonist on the excitability of medial vestibular nuclear neurons. Neurosci Res Communications 35: 139-149, 2004 https://doi.org/10.1002/nrc.20028
  9. Kay AR, Wong RK. Isolation of neurons suitable for patch-clamping from adult mammalian central nerveous system. J Neurosci Methods 16: 227-238, 1986 https://doi.org/10.1016/0165-0270(86)90040-3
  10. Cameron SA, Dutia MB. Lesion-induced plasticity in rat vestibular nucleus neurones dependent on glucocorticoid receptor activation. J Physiol 518: 151-158, 1999 https://doi.org/10.1111/j.1469-7793.1999.0151r.x
  11. Zennou-Azogui Y, Borel L, Lacour M, Ez-Zaher L, Ouaknine M. Recovery of head postural following unilateral vestibular neuroectomy in the cat. Acta Otolaryngol (Stockh) 509: 1-19, 1993
  12. Mansour A, Fox CA, Burke S, Meng F, Thompson RC, Akil H, Watson SJ. Mu, delta and kappa opioid receptor mRNA expression in the rat CNS: An in situ hybridzation study. J Comp Neurol 350: 413-438, 1994
  13. Park JS, Jeong HS. Effects of nitric oxide on the vestibular functional recovery after unilateral labyrinthectomy. Jpn J Pharmacol 84: 425-430, 2000 https://doi.org/10.1254/jjp.84.425
  14. Yamanaka T, Sasa M, Amano T, Miyahara H, Matsunaga T. Role of glucocorticoid in vestibular compensation in relation to activation of vestibular nucleus neurons. Acta Otolaryngol (Stockh) 519: 168-172, 1995
  15. Crain SM, Shen KF. Opioids can evoke direct receptor-mediated excitatory effects on sensory neurons. Trends Pharmacol Sci 11: 77-81, 1990 https://doi.org/10.1016/0165-6147(90)90322-Y
  16. Sulaiman MR, Dutia MB. Opioid inhibition of rat medial vestibular nucleus neurones in vitro and its dependence on age. Exp Brain Res 122: 196-202, 1998 https://doi.org/10.1007/s002210050507
  17. de Waele C, Muhlethaler M, Vidal PP. Neurochemistry of the central vestibular pathways. Brain Res Rev 20: 24-46, 1995 https://doi.org/10.1016/0165-0173(94)00004-9
  18. Smith PF, Darlongton CL. Neurochemical mechanisms of recovery from peripheral vestibular lesions (vestibular compensation). Brain Res Rev 16: 117-133, 1991 https://doi.org/10.1016/0165-0173(91)90001-O
  19. Serafin M, de Waele C, Khateb A, Vidal PP, Muhlethaler M. Medial vestibular nucleus in the guinea-pig. Ionic basis of the intrinsic membrane properties in brainstem slices. Exp Brain Res 84: 426- 433, 1991. https://doi.org/10.1007/BF00231465
  20. Johnston AR, MacLeod NK, Dutia MS. Ion conductances contributing to spike repolarization and afterpotential in rat medial vestibular nucleus neurons. J Physiol Lond 481: 61-77, 1994
  21. Kim HW, Park JS, Jeong HS, Jang MJ, Kim BC, Kim MK, Cho KH, Kim TS, Park SW. Nitric oxide modulation of the spontaneous firing of rat medial vestibular nuclear neurons. J Pharmacol Sci 96: 224-228, 2004 https://doi.org/10.1254/jphs.SCJ04006X
  22. Peusner KD, Gamkrelidze G, Giaume C. Potassium currents and excitability in second-order auditory and vestibular neurons. J Neurosci Res 53: 511-520, 1998 https://doi.org/10.1002/(SICI)1097-4547(19980901)53:5<511::AID-JNR1>3.0.CO;2-C