[$Ca^{2+}-induced$ $Ca^{2+}$ Release from Sarcoplasmic Reticulum Negatively Regulates Myocytic ANP Release in Beating Rabbit Atria

  • Li, Dan (Department of Physiology, Institute for Medical Sciences, Institute for Basic Sciences, Jeonbug National University Medical School) ;
  • Quan, He Xiu (Department of Physiology, Institute for Medical Sciences, Institute for Basic Sciences, Jeonbug National University Medical School) ;
  • Wen, Jin-Fu (Department of Physiology, Institute for Medical Sciences, Institute for Basic Sciences, Jeonbug National University Medical School) ;
  • Jin, Jing-Yu (Department of Physiology, Institute for Medical Sciences, Institute for Basic Sciences, Jeonbug National University Medical School) ;
  • Park, Sung-Hun (Department of Physiology, Institute for Medical Sciences, Institute for Basic Sciences, Jeonbug National University Medical School) ;
  • Kim, Sun-Young (Department of Physiology, Institute for Medical Sciences, Institute for Basic Sciences, Jeonbug National University Medical School) ;
  • Kim, Sung-Zoo (Department of Physiology, Institute for Medical Sciences, Institute for Basic Sciences, Jeonbug National University Medical School) ;
  • Cho, Kyung-Woo (Department of Physiology, Institute for Medical Sciences, Institute for Basic Sciences, Jeonbug National University Medical School)
  • Published : 2005.04.21


It is not clear whether $Ca^{2+}-induced$ $Ca^{2+}$ release from the sarcoplasmic reticulum (SR) is involved in the regulation of atrial natriuretic peptide (ANP) release. Previously, we have shown that nifedipine increased ANP release, indicating that $Ca^{2+}$ entry via voltage-gated L-type $Ca^{2+}$ channel activation decreases ANP release. The purpose of the present study was two-fold: to define the role of SR $Ca^{2+}$ release in the regulation of ANP release and whether $Ca^{2+}$ entry via L-type $Ca^{2+}$ channel is prerequisite for the SR-related effect on ANP release. Experiments were performed in perfused beating rabbit atria. Ryanodine, an inhibitor of SR $Ca^{2+}$ release, increased atrial myocytic ANP release ($8.69{\pm}3.05$, $19.55{\pm}1.09$, $27.31{\pm}3.51$, and $18.91{\pm}4.76$% for 1, 2, 3, and $6{\mu}M$ ryanodine, respectively; all P<0.01) with concomitant decrease in atrial stroke volume and pulse pressure in a dose-dependent manner. In the presence of thapsigargin, an inhibitor of SR $Ca^{2+}$ pump, ryanodine-induced increase in ANP release was not observed. Thapsigargin attenuated ryanodine-induced decrease in atrial dynamic changes. Blockade of L-type $Ca^{2+}$ channel with nifedipine abolished ryanodine-induced increase in ANP release ($0.69{\pm}5.58$% vs. $27.31{\pm}3.51$%; P<0.001). In the presence of thapsigargin and ryanodine, nifedipine increased ANP release and decreased atrial dynamics. These data suggest that $Ca^{2+}$-induced $Ca^{2+}$ release from the SR is inversely involved in the regulation of atrial myocytic ANP release.


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