Effects of Duchesnea indica on Human Stomach Cancer Cells(KATOIII)

사매의 KATOIII 위암세포(胃癌細胞)에 대(對)한 항암효능(抗癌效能)

  • Lim, Joong-Hwa (3rd Dept. of Internal Medicine, College of Oriental Medicine, Kyung-Hee University) ;
  • Kim, Jin-Sung (3rd Dept. of Internal Medicine, College of Oriental Medicine, Kyung-Hee University) ;
  • Yoon, Sang-Hyub (3rd Dept. of Internal Medicine, College of Oriental Medicine, Kyung-Hee University) ;
  • Ryu, Ki-Won (3rd Dept. of Internal Medicine, College of Oriental Medicine, Kyung-Hee University) ;
  • Ryu, Bong-Ha (3rd Dept. of Internal Medicine, College of Oriental Medicine, Kyung-Hee University)
  • 임중화 (경희대학교 한의과대학 비계내과학교실) ;
  • 김진성 (경희대학교 한의과대학 비계내과학교실) ;
  • 윤상협 (경희대학교 한의과대학 비계내과학교실) ;
  • 류기원 (경희대학교 한의과대학 비계내과학교실) ;
  • 류봉하 (경희대학교 한의과대학 비계내과학교실)
  • Published : 2005.06.30

Abstract

Background : Death by cancer, malignant tumor, is on the increase around the world. In South Korea, cancer is the number one cause of death, and, in South Korea, stomach cancer is the most common form of cancer. This may be due to genetic and enviornmental factors. Because stomach cancer is so prevalent here, this study was undertaken. Objectives: This study was conducted to investigate the anti-cancer effects of Duchesnea indica(Andr.) Focke. in human stomach cancer cells(KATOIII). Materials & Methods: For human stomach cancer cells KATO III cells were obtained from Korea Cell Line Bank. The water decoction of Duchesnea indica(Andr.) Focke was added to cultures. First, morphological changes were examined under an inverted microscope. The destruction of stomach cancer cells was measured through Trypan blue exclusion testing, and suppression of viability of stomach cancer cells was measured via MTT assay. Anti-cancer mechanisms were assessed by analyzing the cell cycle. Results & Conclusions : In morphologic change, stomach cancer cells showed the withdrawn and floating appearance that is typical in cellular impairment. In each test group more stomach cancer cells were killed than in the control group to a statistically significant degree. Each test group showed more suppression of viability of stomach cancer cells than that of the control group to a statistically significant degree. Analysis of the cell cycle showed that Duchesnea indica(Andr.) Focke inhibited division of stomach cancer cells. This experiment yielded empirical evidence for Duchesnea indica(Andr.) Focke as a statistically significant anticarcinogen, specifically against stomach cancer.