Occurrence of the B- and Q-biotypes of Bemisia tabaci in Korea

담배가루이 B와 Q 계통의 국내 발생 상황

  • 이민호 (농업과학기술원 농업해충과) ;
  • 강석영 (농업과학기술원 농업해충과) ;
  • 이선영 (농업과학기술원 농업해충과) ;
  • 이흥수 (경남농업기술원 식물환경연구과) ;
  • 최준열 (농업과학기술원 농업해충과) ;
  • 이관석 (농업과학기술원 농업해충과) ;
  • 김황용 (농업과학기술원 농업해충과) ;
  • 이시우 (농업과학기술원 농업해충과) ;
  • 김선곤 (전남농업기술원) ;
  • 엄기백 (농업과학기술원 농업해충과)
  • Published : 2005.09.01


The occurrence of tobacco whiteflies, Bemisia tabaci, in greenhouses was monitored in Korea in 2005. Bemisia tabaci occurred in the rose, sweet pepper, tomato, and cucumber greenhouses of Chungbuk, Chungnam, Gyongnam, and Jeonnam Provinces, but not in Jeonbuk and Gyongbuk Provinces. The biotypes and genetic differentiation of the whiteflies collected in each regions were analyzed by mitochondrial 16S DNA sequences. The 16S DNA sequences of Jincheon (Chungbuk Province) samples were similar to DNA data reported from Japan and Israel which were known as the B biotype. However, the DNA sequences of the Buyeo (Chungnam), Geoje (Gyongnam) and Boseong (Jeonnam) collections, which were 100% homologous showed over 99% similarity to the DNA of Q biotype from Spain and Egyrt. Here we report the first founding of the Q biotype in Korea. It is assumed that, unlike the B biotype reported from Jincheon since 1998, the Q biotype might have been introduced recently from the certain foreign region/country to the greenhouses in those provinces.


  1. EPPO. 2004. Diagnostic protocols for regulated pests. Bemisia tabaci. EPPO Bulletin 34: 281-288 https://doi.org/10.1111/j.1365-2338.2004.00729.x
  2. Kimura, M. 1980. A simple method for estimating evolutionary rate of base substitution through comparative studies of nucleotide sequences. J. Mol. Evol. 16: 111-120 https://doi.org/10.1007/BF01731581
  3. Lee, M.L. and P.J. De Barro. 2000. Characterization of different biotypes of Bemisia tabaci (Gennadius) (Homoptera: Aleyrodidae) in South Korea on 16S ribosomal RNA sequences. Korean J. Entomol. 30: 125-130
  4. Lee, M.L., S.B. Ahn and W.S. Cho. 2000. Morphological characteristics of Bemisia tabaci (Gennadius) (Homoptera: Aleyrodidae) and discrimination of their biotypes in Korea by DNA markers. Korean J. Appl. Entomol. 39: 5-12
  5. Moya, A., P. Guirao, D. Cifuentes, F. Beitia and J.L. Cenis. 2001. Genetic diversity of Iberian populations of Bemisia tabaci (Hemiptera: Aleyrodidae) based on random amplified polymorphic DNA-polymerase chain reaction. Mol. Ecol. 10: 891-897 https://doi.org/10.1046/j.1365-294X.2001.01221.x
  6. Perring, T.M. 2001. The Bemisia tabaci species complex. Crop Protection 20: 725-737 https://doi.org/10.1016/S0261-2194(01)00109-0
  7. Burban, C., L.D.C. Fishpool, C. Fauquet, D. Fargette and J.C. Thouvenel. 1992. Host associated biotypes within West African population of the whitefly Bemisia tabaci (Genn.) (Hom., Aleyrodidae). J. Appl. Entomol. 113: 416-423 https://doi.org/10.1111/j.1439-0418.1992.tb00682.x
  8. Sambrook, J. and D.W. Russell. 2001. Molecular cloning a laboratory manual. Cold Spring Harbor Lab. Press, Cold Spring Harbor, New York
  9. Honda, K. 2005. The present status and control strategies of tomato yellow leaf curl virus. Kongetsu no Nogyo, Feb. pp. 15-19
  10. Jones, D. 2003. Plant viruses transmitted by whiteflies. Eur. J. Plant Pathol. 109: 197-221
  11. Muniz, M. and G. Nombela. 2001. Differential variation in development of the B- and Q-biotypes of Bemisia tabaci (Homoptera: Aleyrodidae) on sweet pepper at constant temperatures. Environ. Entomol. 30: 720-727 https://doi.org/10.1603/0046-225X-30.4.720
  12. Thompson, J.D., D.G. Higgins and T.J. Gibson. 1994. Clustal W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position specific gap penalties and weight matrix choice. Nucleic Acids Res. 22: 4673-4680 https://doi.org/10.1093/nar/22.22.4673
  13. Zhang, L.P., Y.J. Zhang, W.J. Zhang, Q.J. Wu, B.Y. Xu and D. Chu. 2005. Analysis of genetic diversity among different geographical populations and determination of biotyes of Bemisia tabaci in China. JEN 129: 121-128
  14. Nombela, G., F. Beitia and M. Muniz. 2001. A differential interaction study of Bemisia tabaci Q-biotype on commercial tomato varieties with and without the Mi resistance gene, and comparative host responses with the B-biotype. Entomol. Exp. Appl. 98: 339-344 https://doi.org/10.1023/A:1018911114463
  15. Gennadius, P. 1889. Disease of the tobacco plantations in the Trikonia. The alcurodid of tobacco. Ellenike Georgia 5: 1-3
  16. Guirao, P., F. Beitia and J.L. Cenis. 1997. Biotype determination in Spanish populations of Bemisia tabaci (Hemiptera: Aleyrodidae). Bull. Entomol. Res. 87: 587-593 https://doi.org/10.1017/S0007485300038682
  17. Costa, H.S., D.E. Ullman, M.W. Johnson and B.E. Tabashnik. 1993. Squash silverleaf symptoms induced by immature, but not adult, Bemisia tabaci. Phytopathology 83: 763-766 https://doi.org/10.1094/Phyto-83-763
  18. Elbert, A. and R. Nauen, 2000. Resistance of Bemisia tabaci (Homoptera: Aleyrodidae) to insecticides in southern Spain with special reference to neonicotinoids. Pest Management Sci. 56: 60-64 https://doi.org/10.1002/(SICI)1526-4998(200001)56:1<60::AID-PS88>3.0.CO;2-K
  19. Pascual, S. and C. Callejas. 2004. Intra- and interspecific competition between biotypes Band Q of Bemisia tabaci (Hemiptera: Aleyrodidae) from Spain. Bull. Entomol. Res. 94: 369-375
  20. Perring, T.M., A.D. Cooper, R.J. Rodriguez, C.A. Farrar and T.S. Bellows. 1993. Identification of a whitefly species by genomic and behavioral studies. Science 259: 74-77 https://doi.org/10.1126/science.8418497
  21. Simon, C., F. Frati, A. Beckenbach, B. Crespi, H. Liu and P. Flook. 1994. Evolution, weighting, and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers. Ann. Entomol. Soc. Am. 87: 651-701 https://doi.org/10.1093/aesa/87.6.651
  22. Beitia, F., I. Mayo, E.M. Robles-Chillida, P. Guirao, J.L. Cenis. 1997. Current status of Bemisia tabaci (Gennadius) in Spain: the presence of biotypes of this species. Bulletin of the OILB/SROP 20: 99-107
  23. Cervera, M.T., J.A. Cabezas, B. Simon, J.M. Martinez-Zpapter, F. Beitia and J.L. Cenis. 2000. Genetic relationships among biotypes of Bemisia tabaci (Hemiptera: Aleyrodidae) based on AFLP analysis. Bull. Entomol. Res. 9: 391-396
  24. Ueda, S., T. Kimura, M. Onuki, K. Handa and T. Iwanami. 2004. Three distinct groups of isolates of Tomato yellow leaf curl virus in Japan and construction of an infectious clone. J. Gen. Plant Pathol. 70: 232-238 https://doi.org/10.1007/s10327-003-0112-5
  25. Bedford, I.D., R.W. Briddon, J.K. Brown, R.C. Rosell and P.G. Markham. 1994. Geminivirus transmission and biological characterisation of Bemisia tabaci (Gennadius) biotypes from different geographic regions. Ann. Appl. Biol. 125: 311-325 https://doi.org/10.1111/j.1744-7348.1994.tb04972.x
  26. Bellows, T.S., T.M. Perring, R.J. Gill and D.H. Headrick. 1994. Description of a species of Bemisia. Ann. Entomol. Soc. Am. 87: 195-206 https://doi.org/10.1093/aesa/87.2.195
  27. Byrne, D.N. 1999. Migration and dispersal by the sweet potato whitefly, Bemisia tabaci. Agri. Forest Meteorol. 97: 309-316 https://doi.org/10.1016/S0168-1923(99)00074-X
  28. Nauen, R., N. Stumpf and A. Elbert. 2002. Toxicological and mechanistic studies on neonicotinoid cross resistance in Q-type Bemisia tabaci. Pest Management Sci. 58: 868-875 https://doi.org/10.1002/ps.557