Isolation of Pigment Overproducing Mutant from Monascus purpureus and Optimization of Pigment Production

Monascus purpureus로부터 다량의 색소생성 변이주의 분리 및 색소생성의 최적조건

  • Park Chi Duck (Department of Microbiology, Keimyung University) ;
  • Jung Hyuck Jun (Department of Microbiology, Keimyung University) ;
  • Yu Tae Shick (Department of Microbiology, Keimyung University)
  • 박치덕 (계명대학교 미생물학과) ;
  • 정혁준 (계명대학교 미생물학과) ;
  • 유대식 (계명대학교 미생물학과)
  • Published : 2005.06.01


Isolation of a pigment overproducing mutant, P-57, by ultraviolet irradiation of Monascus purpureus KCCM 60016 and investigation of the optimal conditions for pigment production of the mutant were carried out. P-57 mutant produced pigment on solid state culture. Unpolished rice was the best cereal source for pigment production among eight kinds of tested cereal sources for the solid culture of the mutant. The optimal culture condition for pigment production were obtained from the cultivated at $30^{\circ}C,\;90\%$ humidity for 30 days. The P-57 mutant strain showed the best pigment productivity of 160.0 unit at red pigment, 193.6 unit at orange pigment, and 141.6 unit at yellow pigment on solid state culture under optimal condition.


  1. Kim, H.S., H.S. Kwak, H.S. Yang, Y.R. Pyun, and J.H. Yu. 1979. Stuides on the pigment produced by Monascus sp. in submerged culture. Kor. J. Appl. Microbiol. Bieoeng. 7, 31-36
  2. Lee, B.K., N.H. Park, H.Y. Pyao, and W.J. Chung. 2001. Production of red pigments by Monascus purpureus in submerged culture. Biotechnol. Bioprocess Eng. 6, 341-346
  3. Lotong, N., and P. Suwanarit. 1990. Fermentation of ang-kak in plastic bags and regulation of pigmentation by initial moisture content. J. Appl. Bacteriol. 68, 565-570
  4. Ryu, H.S., B.H. Lee, B.G. Park, H.S. Kim, H.S. Kim and J.H. Lee. 1989. Production of red pigment by mutants of Monascus anka. Korean J. Food Sci. Technol. 21, 31-36
  5. Manzoni, M. and M. Rollini. 2002. Biosynthesis and biotechnological production of strain by filamentous fungi and application of these cholesterol-lowing drugs. Appl. Microbiol. Biotechnol.58, 555-564
  6. Yoshimura, M., Y. Shigeru, K. Mitsugi, and H. Yoshio. 1975. Production of Monascus-pigment in a submerged culture. Agr. Biol. Chem. 39, 1789-1795
  7. Martinkova. L., P. Juzlova, and D. Vesely. 1995. Biological activity of polyketide  pigments produced by the fungus Monascus. J. Appl. Bacteriol. 79, 609-616
  8. Yu, T.S. 1999. Hong-ju and pigments produced by filamentous fungus Monascus. J. Inst. Nat. Sci. Keimyung University. 18, 87- 92
  9. Yongsmith, B., V. Kitprechavanichi, L. Chitradon, C. Chaisrisook, and N. Budda. 2000. Color mutants of Monascus sp. KB9 and their comparative glucoamylase on rice solid culture. J. Mol. Catal. B: Enzym. 10, 263-272
  10. Park, H.E., C.H. Kim, and K.H. Min. 1991. Isolation of pigment- producing mutants from Monascus sp. KS2 and optimization of cultural conditions. Kor. J. Mycol. 19, 120-127
  11. Frazier, W.C. 1967. Food Microbiology, McGraw-Hill Book Co., New York, p. 23, 411
  12. Kim, S. Y., and J. K. Kim. 1990. Pigment production in Monascus anka. J. Korean Agric. Chem. Soc. 3, 239-246
  13. Krairak, S., K. Yamamura, R. Irie, M. Nakajima, H. Shimizu, P. Anage, B. Yongsmith, and S. Shioya. 2000. Maximizing yellow pigment production in fed-betch culture of Monascus sp. J. Biosi. Bioeng. 90, 363-367
  14. Yuan, C.S. 1983. Fermentative production of anka-pigments. Kor. J. Appl. Microbiol. Bieng. 11, 325-337
  15. Saito, K. 1910. Notizen uber einige koreanische garungsorganismen. Cent. f. Bakt. II Abt. Bd., 26, 369-373
  16. Lee, Y.K.,D.C.Chen, S. Chauvatcharin, T. Seki, and T. Yoshida. 1995. Production of Monascus pigments by a soild-liquid state culture method. J. Ferment. Bio. 79, 516-518
  17. Wang, H.C., and T.S. Bau. 1977. Pigmentation and antibacterial activity of fast neutron and X-ray-induced strains of Monascus purpureus Went. Plant Physiol. 69, 578-581
  18. Jung, H.J. and T.S. Yu 2004. Production of cell mass and monacolin K from Monascus sp. on rice solid culture. Kor. J. Microbiol. 40, 160-166