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Effects of Ginsenoside Total Saponins on Experimental Irritable Bowel Syndrome in Rats

  • Kim, Jong-Hoon (Research Laboratory for the Study of Ginseng Signal Transduction and Dept. of Physiology, College of Veterinary Medicine, Konkuk University) ;
  • Nah, Seung-Yeol (Research Laboratory for the Study of Ginseng Signal Transduction and Dept. of Physiology, College of Veterinary Medicine, Konkuk University)
  • Published : 2005.06.01

Abstract

In the previous study, we reported that the in viかo inhibitory effect of ginsenosides, active ingredient of Panax ginseng, on $5-HT_{3A}$ receptor channel activity is coupled to in vivo anti-vomiting and anti-nausea effect. In the present study, we further investigated that the inhibitory effect of ginsenosides, active ingredient of Panax ginseng, on 5-HT3A receptor channel activity is also coupled to attenuation of irritable bowel syndrome (IBS), which is induced by colorectal distention (CRD) and $0.6\%$ acetic acid treatment. The CRD-induced visceral pains induced by CRD and acetic acid treatment are measured by frequency of contractions of the external oblique muscle in conscious rats. Treatment of GTS significantly inhibited CRD-induced visceral pain with dose-dependent manner. The $EC_{50}$ was $5.5{\pm}4.7$ mg/kg ($95\%$ confidence intervals: 1.2-15.7) and the antinociceptive effect of GTS on visceral pain was persistent for 4 h. We also compared the effects of protopanaxadiol (PD) ginsenosides and protopanaxatriol (PT) ginsenosides with saline on acetic acid-and CRD-induced visceral pain, and found that protopanaxatriol (PT) ginsenosides was much more potent than PD ginsenosides in attenuating CRD-induced visceral pain. These results indicate that U ginsenosides of Panax ginseng are components far attenuation of experimentally CRD-induced visceral pains.

References

  1. Camilleri, M., Northcutt, A.R., Kong, S., Dukes, G.E., McSorley, D. and Mangel, A.W. : Efficacy and safety of alosetron in women with irritable bowel syndrome: a randomised, placebo-controlled trial. Lancet. 355, 1035-1040 (2000) https://doi.org/10.1016/S0140-6736(00)02033-X
  2. Prior, A. and Read, N.W : Reduction of rectal sensitivity and post-prandial motility by granisetron, a 5-$HT_3$-receptor antagonist, in patients with irritable bowel syndrome. Aliment. Pharmacol. Ther. 7, 175-180 (1993) https://doi.org/10.1111/j.1365-2036.1993.tb00087.x
  3. Hammer, J., Phillips, S.P., Talley, N.J. and Camilleri, M : Effect of 5-$HT_3$-antagonist (ondansetron) on rectal sensitivity and compliance in health and the irritable bowel syndrome. Aliment. Pharmacol. Ther. 7, 543 (1993)
  4. Talley, N.J : 5-hydroxytryptamine agonists and antagonists in the modulation of gastrointestinal motility and sensation: clinical implications. Aliment. Pharmacol. Ther. Review article 6, 273 (1992) https://doi.org/10.1111/j.1365-2036.1992.tb00050.x
  5. Abe, T : Clinical studies of the vitamins. Jpn. Soc. Inter. Med. 54, 989-1006 (1965) https://doi.org/10.2169/naika.54.989
  6. Attele, A. S., Wu, J. A. and Yuan, C. S : Ginseng pharmacology: multiple constituents and multiple actions. Biochem. Pharmacol. 58, 1685- I 693 (1999) https://doi.org/10.1016/S0006-2952(99)00212-9
  7. Kudo, K., Tachikawa, E., Kashimoto, T. and Takahashi, E : Properties of ginseng saponin inhibition of catecholamine secretion in bovine chromaffin cells. Eur. J. Pharmacol. 341, 139-144 (1998) https://doi.org/10.1016/S0014-2999(97)01350-2
  8. Choi, S., Jung, S. Y., Lee, J. H., Sala, F., Criado, M., Mulet, J., Valor, L. M., Sala, S., Engel, A. G and Nah, S. Y.: Effects of ginsenosides, active components of ginseng, on nicotinic acetylcholine receptors expressed in Xenopus oocytes. Eur. J. Pharmacol. 442,37-45 (2002) https://doi.org/10.1016/S0014-2999(02)01508-X
  9. Sala, F., Mulet, J., Choi, S., Jung, S. Y., Nah, S. Y., Rhim, H., Valor, L. M., Criado, M. and Sala, S : Effects of ginsenoside Rg2 on human neuronal nicotinic acetylcholine receptors. J. Pharmacol. Exp. Ther. 301, 1052-1059 (2002) https://doi.org/10.1124/jpet.301.3.1052
  10. Lee, B.H., Jeong, S.M., Lee, J.H., Kim, D.H., Kim, J.H., Kim, J.I., Shin, H.C., Lee, S.M. and Nah, S.Y : Differential effect of ginsenoside metabolites on the 5-$HT_3$A receptor- mediated ion current in Xenopus oocytes. Mol Cells. 29, 17(1),51-56 (2004) https://doi.org/10.1007/s10059-010-0016-0
  11. Langlois, A., Pascaud, X., Junien, J.L., Dahl, S.G and Riviere, P.J: Response heterogeneity of 5-$HT_3$ receptor antagonists in a rat visceral hypersensitivity model. Eur J Pharmacol. 318, 141-144 (1996) https://doi.org/10.1016/S0014-2999(96)00857-6
  12. Litchfield, J.T., and Wilcoxon, F.: A simplified method of evaluating dose-effect experiments. J Pharmacol. Exp. Ther. 96-99 (1949)
  13. Moss, H.E. and Sanger, G.J : The effects of granisetron, lCS 205-930 and ondansetron on the visceral pain reflex induced by duodenal distension. Br. J Pharmacol. 100, 497-501 (1990) https://doi.org/10.1111/j.1476-5381.1990.tb15836.x
  14. Banner, S.E. and Sanger, G.J : Differences between 5-$HT_3$receptor antagonists in modulation of visceral hypersensitivity. Br. J. Pharmacol. 114, 558-562 (1995) https://doi.org/10.1111/j.1476-5381.1995.tb13263.x

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