Effect of Tea Catechin, EGCG (Epigallocatechin Gallate) on Killing of Oral Bacteria

차 카테킨 EGCG (Epigallocatechin Gallate)의 구강세균에 대한 살균효과

  • Yu Mi-Ok (Department of Life Science, Soonchun­hyang University) ;
  • Chun Jae-Woo (Department of Life Science, Soonchun­hyang University) ;
  • Oh Kye-Heon (Department of Life Science, Soonchun­hyang University)
  • 유미옥 (순천향대학교 자연과학대학 생명과학부) ;
  • 천재우 (순천향대학교 자연과학대학 생명과학부) ;
  • 오계헌 (순천향대학교 자연과학대학 생명과학부)
  • Published : 2004.12.01


The purpose of this work was to investigate the effect oftea catechin, epigallocatechin gallate (EGCG) on killing of oral bacteria. The antibacterial activity of 2.5 mg/ml and 5.0 mg/ml EGCG was investigated for target bacteria of which initial cell number was approximately adjusted to $10^{7}ml$. The antibacterial activity of EGCG was proportional to the concentration according to colony-forming unit(CFU) of target bacteria enumerating on selective and complex media. Streptococcus mutans and Streptococcus sobrinus at 5mg/ml EGCG were completely killed within 8 hrs. Lactobacillus plantarum and Lactobacillus acidophilus were also killed within 2 hrs and 4 hrs under the same conditions, respectively. Oral bacteria at 2.5 mg/ml EGCG were completely killed within 10 hr. Colony numvers of S. mitis and S. salivarius treated with 2.5 mg/ml EGCG were decreased on MS solid media and no colony was observed on the media within 12 hrs. In consequence, EGCG would be a natural and effective compound that kill oral bacteria being caused of bad breath, plaque and gingivitis, and for preventing and treating dental caries.


EGCG;oral bacteria;tea catechin


  1. Cho, Y.S., H.Y. Kahng, C.K. Kim, J.J. Kukor, and K.H. Oh. 2002. Physiological and cellular responses of the 2,4-D degrading bacterium, Burkholderia cepacia YK-2, to the phenoxyherbicides 2,4-D and 2,4,5-T. Curr. Microbiol. 415-422
  2. Grahm, H.N. 1992. Green tea composition, consumption, and polyphenol chemistry. Prev. Med. 21, 334-350
  3. Hamilton-Miller, J.M. 1995. Antimicrobial properties of tea (Camella sinensis, L). Antimicrob. Agents Chemother. 39, 2375- 2377
  4. Hattori, M., I.T. Kusumoto, T. Namba, T. Ishigami, and Y. Hara. 1990. Effect of tea polyphenols on glucan synthesis by glucosyltransferase from Streptococcus mutans. Chem. Pharm. Bull. 38, 717-720
  5. Hu, Z.Q., W.H. Zhao, N. Asano, Y. Yoda, Y. Hara, and T. Shimamura. 2002. Epigallocatechin gallate synergistically enhances the activity of carbapenems against methicillin-resistant Staphylococcus aureus. Antimicrob. Agents Chemother. 46, 558-560
  6. Katsuhiro, K., M. Yanada, I. Oguni, and T. Takahashi. 1999. In vitro and in vivo activities of tea catechin against Helicobacter pylori. Antimicrob. Agents Chemother. 43, 1788-1791
  7. Nagano, J., K. Suminori, Kono, D.L. Preston and K. Mabuchi. 2001. A prospective study of green tea consumption and cancer incidence, Hiroshima and Nagasaki (Japan). Cancer Cause & Control 12, 501-508
  8. Zhao, W.H., Z.Q. Hu, Y. Hara, and T. Shimamura. 2002. Inhibition of penicillinase by epigallocatechin gallate resulting in restoration of antibacterial activity of penicillin against penicillinase-producing Staphylococcus aureus. Antimicrob. Agents Chemother. 46, 2266-2268
  9. Leanderson, P., A.O. Faresjoe, C. Tagesson, 1997. Green tea polyphenols inhibit oxidant-induced DNA strand breakage in cultured lung cells. Free Radical Biol. Med. 23, 235-242
  10. Cowan, M.M. 1999. Plant products as antimicrobial agents. Clin. Microbiol. Rev. 12, 564-582
  11. Alessio, H.M., A.E. Hagerman, M. Romanello, S. Carando, M.S. Threlkeld, J. Rogers, Y. Dimitrova, S. Muhanned, and R.L. Wiley. 2002. Comsumption of green tea protects rats from exerciseinduced oxidative stress stress in kidney and liver. Nutri. Res. 22, 1177-1188
  12. Mimoto, J., K. Kiura, K. Matsuo, T. Yoshino, I. Takara, H. Ueoka, M. Kataoka, and M. Harada. 2000. Epigallocatechin gallate can prevent cisplatin-induced lung tumorogenesis in A/J mice. Carcinogenesis 21, 915-919
  13. Kawamura, J. and T. Takeo. 1989. Antibacterial activity of tea catechin to Streptococcus mutans. J. Jpn. Soc. Food Sci. Technol. 36, 463-467
  14. Chang, H.W., M.O. Yu, H.K. Bae, W.S. Choi, and K.H. Oh. 2002. In vitro antimicrobial activity of korean green tea extracts against potential pathogenic microorganisms. Abstracts of 102nd American Society for Microbiology, Salt Lake City, Utah, USA
  15. Riso, P., D. Erba, F. Criscuoli, and G. Testolin. 2002. Effect of green tea extract on DNA repair and oxidative damage due to H$_2$O$_2$ in Jurkat T cells. Nutri. Res. 22: 1143-1150