Development of an Efficient Procedure for the Construction of Metagenomic Library from Environment Samples

효율적인 Metagenomic Library의 제작 방법 탐구

  • Lim Dongbin (Department of Bioinformatics and Life Science, Soongsil University)
  • 임동빈 (숭실대학교 자연과학대학 생명정보학과)
  • Published : 2004.12.01


I investigated an effective way to generate a metagenomic library from DNA prepared from environental samples. The sizes of DNA extracted from environmental samples were usually in the range of 10 to 100 kbp as estimated from $0.4\%$ agarose gel electrophoresis. Because of this small size, a fosmid, rather than BAC, was chosen as a vector. It was found that, for the successful generation of metagenomic library, the selection of DNA with the sized of about 40 kbp was critical and, therefore, a simple agarose gel electrophoresis system was developed to select this size of DNA. By the procedure described in this report, I obtained metagenomic libraries containing 25,000 fosmid clones, which corresponded to 1,000 Mb of metagenomic DNA.


metagenome;metagenomic library;environmental DNA;fosmid


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