Identification of Antagonistic Bacteria, Pseudomonas aurantiaca YC4963 to Colletotri­chum orbiculare Causing Anthracnose of Cucumber and Production of the Antibiotic Phenazine-l-carboxylic acid

Colletotrichum orbiculare에 대한 길항세균 Pseudomonas aurantiaca YC4963의 분리 동정 및 항균물질 Phenazine-1-carboxylic acid의 생산

  • Chae Hee-Jung (Division of Applied Life Sciences (BK21 program), Gyeongsang National University) ;
  • Kim Rumi (Division of Applied Life Sciences (BK21 program), Gyeongsang National University) ;
  • Moon Surk-Sik (Department of Chemistry, Kongju National University) ;
  • Ahn Jong-Woong (Division of Ocean Science, Korea Maritime University) ;
  • Chung Young-Ryun (Division of Applied Life Sciences (BK21 program), Gyeongsang National University)
  • 채희정 (경상대학교 응용생명과학부, 기초과학연구소) ;
  • 김루미 (경상대학교 응용생명과학부, 기초과학연구소) ;
  • 문석식 (공주대학교 화학과) ;
  • 안종웅 (한국해양대학교 해양과학부) ;
  • 정영륜 (경상대학교 응용생명과학부, 기초과학연구소)
  • Published : 2004.12.01

Abstract

A bacterial strain YC4963 with antifungal activity against Colletotrichum orbiculare, a causal organism of cucumber anthracnose was isolated from the rhizosphere soil of Siegesbeckia pubescens Makino in Korea. Based on physiological and biochemical characteristics and 16S ribosomal DNA sequence analysis, the bac­terial strain was identified as Pseudomonas aurantiaca. The bacteria also inhibited mycelial growth of several plant fungal pathogens such as Botrytis cinerea, Fusarium oxysporum and Rhizoctonia solani on PDA and 0.1 TSA media. The antifungal activity was found from the culture filtrate of this isolate and the active compound was quantitatively bound to XAD adsorption resin. The antibiotic compound was purified and identified as phenazine-l-carboxylic acid on the basis of combined spectral and chemical analyses data. This is the first report on the production of phenazine-l-carboxylic acid by Pseudomonas aurantiaca.

Keywords

anthracnose;Colletotrichum orbiculare;phenazine-1-carboxylic acid;Pseudomonas aurantiaca

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