Studies on Biological Activities of the Polysaccharides and Oligosaccharides of Orostachys japonicus

와송 다당체 및 올리고당류의 생리 활성

  • Kim Ki Hoon (Department of Biomedical Laboratory Science and Biohealth Products Research Center, Inje University) ;
  • Kim Eun Young (Department of Biomedical Laboratory Science and Biohealth Products Research Center, Inje University) ;
  • Kim Yea Oon (Department of Biomedical Laboratory Science and Biohealth Products Research Center, Inje University) ;
  • Baek Geum Ok (Department of Biomedical Laboratory Science and Biohealth Products Research Center, Inje University) ;
  • Kim Han Bok (Department of Life Science, Hoseo University) ;
  • Lee Dong Seok (Department of Biomedical Laboratory Science and Biohealth Products Research Center, Inje University)
  • 김기훈 (인제대학교 의생명공학대학 임상병리학과, 인제대학교 바이오헬스 소재 연구센터) ;
  • 김은영 (인제대학교 의생명공학대학 임상병리학과, 인제대학교 바이오헬스 소재 연구센터) ;
  • 김예운 (인제대학교 의생명공학대학 임상병리학과, 인제대학교 바이오헬스 소재 연구센터) ;
  • 백금옥 (인제대학교 의생명공학대학 임상병리학과, 인제대학교 바이오헬스 소재 연구센터) ;
  • 김한복 (호서대학교 자연과학부 생명과학전공) ;
  • 이동석 (인제대학교 의생명공학대학 임상병리학과, 인제대학교 바이오헬스 소재 연구센터)
  • Published : 2004.12.01


Polysaccharides were prepared from Orostachys japonicus by extration with hot steam water (OJPl). The OIPl fraction was further purified by Sephadex G-50 gel filtration chromatography to produce FI (polysaccharides) and FII (oligosaccharides) fraction. The average molecular masses o fFI and FII fraction were determined to be 3050 kDa and 13 kDa, respectively. The antimicrobial activity of OIPl was tested against 8 strains of bacteria and one strain of yeast by the disc diffusion method, fluorescein diacetate (FDA) method and broth dilution method. The OIPl exhibited a very strong growth inhibition to Candida albicans. The OIPl remarkably sup­pressed the growth of Salmonella typhimurium and Staphylococcus aureus. The OIPl showed higher growth inhibition to Escherichia coli and Pseudomonas aeruginosa than propolis, positive control. When the anticancer activity of the OIPl, FI or FII was examined against human cancer cell lines and the Sarcoma 180 cells, these widely suppressed the proliferation of cell lines in the MTT assay and morphology study. Especially, they remarkably inhibited the growth of A549, HeLa and AGS cells. Also treatment of cancer cells with OJPl, FI or FII induced apoptotic cell death characterized by DNA fragmentation. The OJPl, FI or FII exhibiting various biological activities such as antimicrobial activity and anticancer activity is expected to be developed as new biohealth products.


anticancer activity;antimicrobial activity;DNA fragmentation;oligosaccharides;Orostachys japonicus;polysaccharides


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