Studies on Biological Activities of the Polysaccharides and Oligosaccharides of Orostachys japonicus

와송 다당체 및 올리고당류의 생리 활성

  • Kim Ki Hoon (Department of Biomedical Laboratory Science and Biohealth Products Research Center, Inje University) ;
  • Kim Eun Young (Department of Biomedical Laboratory Science and Biohealth Products Research Center, Inje University) ;
  • Kim Yea Oon (Department of Biomedical Laboratory Science and Biohealth Products Research Center, Inje University) ;
  • Baek Geum Ok (Department of Biomedical Laboratory Science and Biohealth Products Research Center, Inje University) ;
  • Kim Han Bok (Department of Life Science, Hoseo University) ;
  • Lee Dong Seok (Department of Biomedical Laboratory Science and Biohealth Products Research Center, Inje University)
  • 김기훈 (인제대학교 의생명공학대학 임상병리학과, 인제대학교 바이오헬스 소재 연구센터) ;
  • 김은영 (인제대학교 의생명공학대학 임상병리학과, 인제대학교 바이오헬스 소재 연구센터) ;
  • 김예운 (인제대학교 의생명공학대학 임상병리학과, 인제대학교 바이오헬스 소재 연구센터) ;
  • 백금옥 (인제대학교 의생명공학대학 임상병리학과, 인제대학교 바이오헬스 소재 연구센터) ;
  • 김한복 (호서대학교 자연과학부 생명과학전공) ;
  • 이동석 (인제대학교 의생명공학대학 임상병리학과, 인제대학교 바이오헬스 소재 연구센터)
  • Published : 2004.12.01

Abstract

Polysaccharides were prepared from Orostachys japonicus by extration with hot steam water (OJPl). The OIPl fraction was further purified by Sephadex G-50 gel filtration chromatography to produce FI (polysaccharides) and FII (oligosaccharides) fraction. The average molecular masses o fFI and FII fraction were determined to be 3050 kDa and 13 kDa, respectively. The antimicrobial activity of OIPl was tested against 8 strains of bacteria and one strain of yeast by the disc diffusion method, fluorescein diacetate (FDA) method and broth dilution method. The OIPl exhibited a very strong growth inhibition to Candida albicans. The OIPl remarkably sup­pressed the growth of Salmonella typhimurium and Staphylococcus aureus. The OIPl showed higher growth inhibition to Escherichia coli and Pseudomonas aeruginosa than propolis, positive control. When the anticancer activity of the OIPl, FI or FII was examined against human cancer cell lines and the Sarcoma 180 cells, these widely suppressed the proliferation of cell lines in the MTT assay and morphology study. Especially, they remarkably inhibited the growth of A549, HeLa and AGS cells. Also treatment of cancer cells with OJPl, FI or FII induced apoptotic cell death characterized by DNA fragmentation. The OJPl, FI or FII exhibiting various biological activities such as antimicrobial activity and anticancer activity is expected to be developed as new biohealth products.

Keywords

anticancer activity;antimicrobial activity;DNA fragmentation;oligosaccharides;Orostachys japonicus;polysaccharides

References

  1. 金在佶.1984.原色天然藥物 大事典(上卷). 447. 南山堂. 서울
  2. Chee, H.Y. 2002. In vitro Evaluation of the antifungal activity of propolis extract on Cryptococcus neoformans and Candida albicans. Mycology 30, 93-95
  3. Chen, X.M. and G.Y. Tian. 2003. Structural elucidation and antitumor activity of a fructan from Cyathula officinalis Kuan. Carbohydr. Res. 338, 1235-1241
  4. Franz, G. 1989. Polysaccharides in pharmacy: current applications and future concepts. Plant Med. 55, 493-497
  5. Lee, B.W. and D.H. Shin. 1991. Screening of natural antimicrobial plant extract on food spoilage microorganisms. Kor. J. Food Sci. Technol. 23, 200-204
  6. Park, H.J., S.C. Leem, M.S. Lee and H.S. Young. 1994. Triterpene and steroids from Orostachys japonicus. Kor. J. Pharm. 25, 20-23
  7. 左春旭, 仲英, 姜岩靑. 1988. 瓦松 中黃美化合物的 分離鑑定 19, 148. 中草藥. 丁杏包
  8. Lee, S.W., H.J. Kim and S. Hwangbo. 2001. Studies on the chemical characteristics of korean propolis. Kor. J. Food Sci. Ani. Resour. 21, 383-388
  9. Taylor, K.A.C.C. 1995. A modification of the phenol sulfuric acid method of total sugar determination. Appl. Biochem. Biotechnol. 53, 207-214
  10. Chang, R. 2002. Bioactive polysaccharides from traditional chinese medicine herbs as anticancer adjuvants. J. Altern. Complement Med. 8, 559-565
  11. Kim, H.S. and J.O. Shin. 1997. Isolation and antimicrobial activity of Xanthium strumarium L. extract. Kor. J. Appl. Microbiol. Biotechnol. 25, 183-188
  12. Roberfroid, M.B. 1977. Health benefits of non-digestible oligosaccharides. Diet. Fiber Health Dis. 22, 211-219
  13. Chakrabarty, S., M. Roy, B. Hazra and R.K. Bhattacharya. 2002. Induction of apoptosis in human cancer cell lines by diospyrin, a plant-derived bisnaphthoquinonoid, and its synthetic derivatives. Cancer Lett. 188, 85-93
  14. Chand, S., I. Lusunzi, D.A. Veal, L.R. Williams and P. Karuso. 1994. Rapid screening of the antimicrobial activity of extracts and natural products. J. Antibiot. 47, 1295-1304
  15. 左春旭, 蔡玉英, 姜岩靑. 1985.瓦松有效成分的 硏究16, 243. 中草藥. 丁杏包
  16. Ha, Y.L. and W.P. Michael. 1991. Naturally-occuring novel anticarcinogens: Conjugated dienoic derivatives of linoleic acid (CLA) (in Korean). J. Kor. Soc. Food Nutr. 20, 401-407
  17. Park, H.J., H.S. Young, J.O. Kim, S.H. Lee and J.S. Choi. 1991. A study on the chemical constituents of Orostachys japonicus A. Berger. Kor. J. Pharm. 22, 78-84
  18. 小學館. 1985. 小學館編;中藥大事典. 526. 上悔科學技術. 出版社. 上悔
  19. Kono, T., M. Watanabe, K. Koyama, T. Kishimoto, S. Fukushima, T. Sugimura and K. Wakabayashi. 1999. Cytotoxic activity of pierisin, from the cabbage butterfly, Pieris rapae, in various human cancer cell lines. Cancer Lett. 137, 75-81