Hydrolysis Activity of ${\alpha}-Galactosidase$ from Bacillus licheniformis

Bacillus licheniformis로부터 생산된 ${\alpha}-Galactosidase$의 가수분해 활성

  • Kim Hyun Suk (School of Applied Food and Nutritional Science, Woosong University) ;
  • Lee Kyung-Seob (School of Applied Food and Nutritional Science, Woosong University) ;
  • So Jae Ho (School of Applied Food and Nutritional Science, Woosong University) ;
  • Yoon Ki-Hong (School of Applied Food and Nutritional Science, Bioresouce and Application Research Center, Woosong University)
  • 김현숙 (우송대학교, 응용식품 영양학부) ;
  • 이경섭 (우송대학교, 응용식품 영양학부) ;
  • 소재호 (우송대학교, 응용식품 영양학부) ;
  • 윤기홍 (우송대학교, 응용식품 영양학부, 생물소재 응용연구센터)
  • Published : 2004.12.01

Abstract

The maximum productivity of ${\alpha}-galactosidase,$ capable of hydrolyzing completely ${\alpha}-D-l,6-galactopyranosyl$ linkages within oligomeric substrates such as melibiose, raffinose and stachyose to liberate galactose residue, was reached to 718 mU/ml in the culture filtrate of Bacillus licheniformis at death phase. The ${\alpha}-galactosidase$ was identified to show different efficiencies for hydrolyzing the ${\alpha}-galactooligosaccharides$ according to analysis of reaction products by both TLC and quantification of the liberated reducing sugars. The enzyme was active on ${\alpha}-galactooligosaccharides$ in the order of melibiose, raffinose, and stachyose. Though the hydrolyzing activity of enzyme was faintly inhibited by reaction products such as galactose, glucose and sucrose with amounts of five folds more than the added substrates (20 mM), the largest inhibition of enzyme activity was caused by galactose among the end products. Unknown compound, which migrated slower than melibiose on TLC, was detected during hydrolysis reaction of melibiose, suggesting that the ${\alpha}-galactosidase$ has a glycosyl transferase activity. In addition, the enzyme was able to hydrolyze efficiently raffinose and stachyose existed in the soluble extract of soybean meal.

Keywords

Bacillus licheniformis;hydrolysis activity;transferase activity

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