Molecular Characterization of the Bacterial Community in Activated Sludges by PCR­RFLP

PCR-RFLP 방법을 이용한 활성 슬러지의 세균군집 분석

  • Lee Hyun-Kyung (Department of Microbiology and Basic Science Research Institute, Chungbuk National University) ;
  • Kim Jun-Ho (Department of Microbiology and Basic Science Research Institute, Chungbuk National University) ;
  • Kim Chi-Kyung (Department of Microbiology and Basic Science Research Institute, Chungbuk National University) ;
  • Lee Dong-Hun (Department of Microbiology and Basic Science Research Institute, Chungbuk National University)
  • 이현경 (충북대학교 미생물학과 및 기초과학연구소) ;
  • 김준호 (충북대학교 미생물학과 및 기초과학연구소) ;
  • 김치경 (충북대학교 미생물학과 및 기초과학연구소) ;
  • 이동훈 (충북대학교 미생물학과 및 기초과학연구소)
  • Published : 2004.12.01

Abstract

Diversity of the bacterial communities and the relation between community structure and components of waste­water were analyzed by 16S rRNA-based molecular techniques. Clone libraries of the 16S rDNAs from the sludges were constructed by PCR and cloning. The 1,151 clones from a sludge sample of sewage treatment plant were clustered into 699 RFLP phylotypes and the 1,228 clones from the wastewater disposal plant of chemical industry were clustered into 300 RFLP phylotypes. Shannon-Weiner diversity indices of two sampling sites were 8.7 and 6.1, indicating that the bacterial community structure of sewage treatment plant was more diverse than that of wastewater disposal plant of chemical industry. Forty clones belonging to predominant RFLP types were selected and sequenced. Seventy percent (28 clones) of the sequenced clones were related to the uncultured bacteria in public databases. The ${\beta}-Proteobacteria$ dominated in the bacterial communities of investigated two sludge samples. 16S rDNA sequences of the sewage treatment plant were similar to those of other activated sludges, while the bacterial community in wastewater disposal plant of chemical industry rep­resented the strains identified from high-temperature, anaerobic, hydrocarbon-rich, and sulfur-rich environ­ments. This result suggested that bacterial communities depended upon the components of wastewater.

Keywords

16S rRNA;activated sludge;bacterial community;PCR-RFLP

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