Physiology and Growth Properties of Halophilic Bacteria Isolated from Jeotgal (Salted Seafood)

젓갈(염장발효식품)에서 분리한 호염세균의 생리 및 성장특성

  • Jung Yoo Jeong (Department of Biological Engineering, Seokyeong University) ;
  • Park Doo Hyun (Department of Biological Engineering, Seokyeong University)
  • 정유정 (서경대학교 이공대학 생물공학과) ;
  • 박두현 (서경대학교 이공대학 생물공학과)
  • Published : 2004.12.01

Abstract

Two species of halophilic bacteria were isolated from five salted seafoods and identified by 16S rDNA sequenc­ing homology. One was identified as Halomonas subglaciescola and other four strains were belong to Halomo­nas marina. The identity of all isolates with standard organisms was above $95\%.$ H. subglaciescola, H. marina IN, and H. marina SH-2 grew in salinity condition from $3%\;to\;18\%$ NaCl but growth of H. marina SQ and H. marina SH-l grew in salinity environment from $8\%\;to\;17\%.$ Maximum biomass of H. subglaciescola, H. marina IN, H. marina SQ, H. marina SH-1, and H. marina SH-2 growing in LB medium containing $15\%$ NaCl were about 3.2, 4.5, 4.5, 5.7, and 4.2, however the maximum biomass in LB medium containing $5\%$ NaCl were about 2.2, 1.1, 0.7, 0.2, and 2.4 as optical density at 660 nm, respectively. In scanning electron micrograph, unknown material (mucus) attached to outer membrane of all isolates was observed. When mucus isolated from halophilic bacterial cell was added to culture of E. coli, E. coli grew in medium containing $15\%$ NaCl.

Keywords

Halomonas marina;Halomonas subglaciescola;halophilic bacteria;halotolerant;osmoregulation

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