Characterizing Salt Stress Response in a Rice Variety and Its Salt Tolerant Lines Derived from In Vitro Mutagenesis

  • Lee In Sok (Korea Atomic Energy Research Institute) ;
  • Kim Dong Sub (Korea Atomic Energy Research Institute) ;
  • Kang Si Yong (Korea Atomic Energy Research Institute) ;
  • Wi Seung Gon (Korea Atomic Energy Research Institute) ;
  • Jin Hua (Department of Horticulture, Chungnam National Univ., Institute of Biological Resources and Environmental Research, Da Lian Nationalties University) ;
  • Yun PiI-Yong (Department of Horticulture, Chungnam National Univ.) ;
  • Lim Yong Pyo (Department of Horticulture, Chungnam National Univ.) ;
  • Lee Young Il (Korea Atomic Energy Research Institute)
  • Published : 2004.12.01

Abstract

The objectives were to compare the salt tolerance levels in the parental rice cultivar, Dongjinbyeo, and induced mutagenesis derived its lines for plant height, MDA, ATPase, POD, and 2-dimensional protein electrophoresis pattern in NaCl-containing hydroponic nutrient solutions. Rice plants isolated from a population of rice (Oryza sativa L. cv. Dongjinbyeo) mutation lines, which were generated in combination with in vitro selection and gamma-ray, exhibited salt tolerance. Line No. 18 had the longest plant, whereas NaCl-sensitive line (No. 25) had the shortest plant. The parent, and the sensitive line showed severe damage from salt stress. Tolerant lines (No. 18, 50) had a lower malonaldehyde (MDA) content than the sensitive one (Dongjinbyeo, No. 25) during salt stress. Several proteins showed significant quantitative variation through 2DE; phosphoribulokinase, peroxidase, oxygen evolving enhancer 1 and the $H^+-ATPase$, which are known to be involved in salt tolerance. The effect of salt on peroxidase and $H^+-ATPase$ activity in the seedlings of two groups with contrasting genotypes of rice was studied. A greater activity was recorded in the tolerant lines as compared to the sensitive ones (P<0.05, Duncan's test). The results indicate that salt tolerant lines expressed more salt stress-inducible proteins associated with salt tolerance than the sensitive lines during salt stress.

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