Isolation of Chryseobacterium meningosepticum Producing $\beta$-Mannosidase from a Mudfish

미꾸라지로부터 $\beta$-Mannosidase를 생산하는 Chryseobacterium meningosepticum의 분리

  • Kim, Hyeon-Suk (School of Applied Food and Nutritional Science, Woosong University) ;
  • Yun, Gi-Hong (School of Applied Food and Nutritional Science, Bioresouce and Application Research Center, Woosong University)
  • 김현숙 (우송대학교 응용식품 영양학부) ;
  • 윤기홍 (우송대학교 응용식품 영양학부, 생물소재 응용연구센터)
  • Published : 2004.12.01

Abstract

A bacterium producing the $\beta$-mannosidase was isolated from intestine of fresh fish. The isolate YB-29 has been identified as Chryseomeningosepticum on the basis on its 16S rRNA sequence, morphology and biochemical proper The $\beta$-mannosdiase activity was detected in both the culture filtrate and the cell extract of C. meningosepticum YB-29. The $\beta$-mannosidase of culture filtrate showed the maximum activity for hydrolysis of para-nitrophenyl-$\beta$-D-mannopyranoside at pH 5.0-6.0 and 55-$60^{\circ}C$. The enzyme was able to hydrolyze the oligomeric substrates such as mannobiose and mannotriose with higher activity for mannotriose than mannobiose.

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