Amperometric Determination of Anthracycline Antibiotics with the Mercury Film Thin Layer Flow Cell

수은피막 박막흐름전지를 이용한 Anthracycline계 항생제의 전류법 정량

  • 김경은 (상명대학교 자연과학대학 화학과) ;
  • 한영희 (상명대학교 자연과학대학 화학과)
  • Received : 2004.08.16
  • Accepted : 2004.11.24
  • Published : 2004.12.25


The mercury film thin layer flow cell (MFTLFC) which yielded the highest sensitivity for the electrochemical reduction of doxorubicin was constructed by coating the glassy carbon working electrode (GCE; $A=0.208cm^2$) with $5{\mu}L$ of HgO coating solution (0.5% HgO + 0.25% polystyrene/cyclohexanone) and subsequently followed by applying a potential of -0.40 V for 300 sec in the flow stream of an acetate buffer of pH 4.5. The voltammogram of doxorubicin reached the diffusion current plateau at -0.53 V vs. a Ag/AgCl (3 M NaCl) in the MFTLFC. The diffusion current (Id) of doxorubicin at the MFTLFC was 1.7 times greater than the Id obtained at the TLFC employing a bare glassy carbon working electrode. When the peak areas (electric charge) were plotted vs. concentrations of standard anthracyclines, the calibration factors of doxorubicin and daunorubicin were $1.12{\times}10^8{\mu}C/M$ (coefficient of determination; $R^2$: 0.969) and $0.98{\times}10^8{\mu}C/M$> ($R^2$: 0.999), respectively in the concentration range between $1.0{\times}10^{-8}M$ and $1.0{\times}10^{-6}M$.


mercury film thin layer flow cell;doxorubicin;daunorubicin;flow injection analysis


Supported by : 상명대학교


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