Amperometric Determination of Anthracycline Antibiotics with the Mercury Film Thin Layer Flow Cell

수은피막 박막흐름전지를 이용한 Anthracycline계 항생제의 전류법 정량

  • 김경은 (상명대학교 자연과학대학 화학과) ;
  • 한영희 (상명대학교 자연과학대학 화학과)
  • Received : 2004.08.16
  • Accepted : 2004.11.24
  • Published : 2004.12.25

Abstract

The mercury film thin layer flow cell (MFTLFC) which yielded the highest sensitivity for the electrochemical reduction of doxorubicin was constructed by coating the glassy carbon working electrode (GCE; $A=0.208cm^2$) with $5{\mu}L$ of HgO coating solution (0.5% HgO + 0.25% polystyrene/cyclohexanone) and subsequently followed by applying a potential of -0.40 V for 300 sec in the flow stream of an acetate buffer of pH 4.5. The voltammogram of doxorubicin reached the diffusion current plateau at -0.53 V vs. a Ag/AgCl (3 M NaCl) in the MFTLFC. The diffusion current (Id) of doxorubicin at the MFTLFC was 1.7 times greater than the Id obtained at the TLFC employing a bare glassy carbon working electrode. When the peak areas (electric charge) were plotted vs. concentrations of standard anthracyclines, the calibration factors of doxorubicin and daunorubicin were $1.12{\times}10^8{\mu}C/M$ (coefficient of determination; $R^2$: 0.969) and $0.98{\times}10^8{\mu}C/M$> ($R^2$: 0.999), respectively in the concentration range between $1.0{\times}10^{-8}M$ and $1.0{\times}10^{-6}M$.

Keywords

mercury film thin layer flow cell;doxorubicin;daunorubicin;flow injection analysis

Acknowledgement

Supported by : 상명대학교

References

  1. P. W. Buehler, S. J. Robles, G. R. Adami, R. Gajee and A. Negrusz, Chromatographia, 49, 557-561(1999).
  2. R. Ricciarello, S. Pichini, R. Pacifici, I. Altieri, M. Pellegrini, A. Fattorossi and P. Zuccaro, J. Chromatogr. B, 707, 219-225(1998).
  3. Q. Hu, L. Zhang, T. Zhou and Y. Fang, Anal. Chim. Acta, 416, 15-19(2000).
  4. Y. Hahn and J.-Y. Yoo, J. Kor. Chem. Soc., 41, 180-185(1997).
  5. R. G.-M. Carra, A. Sάnchez-Misiego and A. Zirino, Anal. Chem., 67, 4484-4486(1995).
  6. British Pharmacopoeia Commission, 'British Pharmacopoeia', 492, 594, The Stationery Office, London, England, 2000.
  7. 한국 식품 의약품 안전청, '항생물질의약품기준', 315-320, 약업신문사, 한국, 2000.
  8. European Pharmacopoeia Commission, 'European Pharmacopoeia', 706, 784, Council of Europe, Strasbourg, 1997.
  9. L. Álvarez-Cedrón, M. L. Sayalero and J. M. Lanao, J. Chromatogr. B, 721, 271-278(1999).
  10. F. Lachâtre, P. Marquet, S. Ragot, J. M. Gaulier, P. Cardot and J. L. Dupuy, J. Chromatogr. B, 738, 281-291(2000).
  11. Y. Hahn and H. Y. Lee, Arch. Pharm. Res., 27, 31-34(2004).
  12. United States Pharmacopeial Convention, 'U. S. Pharmacopeia & National Formulary', 498, 606, National Publishing, Philadelphia, PA U.S.A., 2000.
  13. T. Lee, K.-C. Chung and J. Park, Electroanalysis, 14, 833-838(2002).
  14. H. P. Wu, Anal. Chem., 66, 3151-3157(1994).