Determination of oxymatrine in Sophora Radix by High Performance Liquid Chromatography

고성능 액체 크로마토그래피를 이용한 고삼 중의 oxymatrine 분석에 관한 연구

  • Received : 2004.04.27
  • Accepted : 2004.06.05
  • Published : 2004.08.25

Abstract

A high performance liquid chromatographic method was designed for the quantitative analysis of oxymatrine in Sophora Radix. The separation of oxymatrine was performed by reversed-phase chromatography with a $C_{18}$ column and a buffered aqueous solution containing acetonitrile, and monitored by UV absorption at 215 nm. Extraction of oxymatrine in Sophora Radix was carried out using various solvents and extraction methods. The optimum extraction efficiency for the crushed Sophora Radix was achieved by reflux at $80^{\circ}C$ in 50% ethanol for five hours. Most extraction methods used to complicate pretreatments. In this study, sublimation was employed for a extraction method without going through complicate pretreatments. Sublimation was carried out under high vacuum ($1{\times}10^{-3}$ torr) and at high temperature ($200^{\circ}C$). Extraction efficiency using Sublimation was found to be inferior to other extraction methods.

Keywords

Oxymatrine;HPLC;Sophora radix

Acknowledgement

Supported by : 숭실대학교

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