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다양한 생물반응기 형태가 인삼(Panax ginseng C.A. Meyer) 부정근의 생장과 Ginsenoside 생산에 미치는 영향

Effects of Various Bioreactors on Growth and Ginsenoside Accumulation in Ginseng Adventitious Root Cultures(Panax ginseng C.A. Meyer)

  • 김윤수 (중앙대학교 인삼산업연구센터) ;
  • 한은주 (충북대학교 첨단원예기술개발연구센터) ;
  • 백기엽 (충북대학교 첨단원예기술개발연구센터)
  • 발행 : 2004.09.01

초록

생물반응기의 형태에 따른 인삼부정근의 생장과 ginsen-side의 생산능력에 대하여 조사한 결과, 원형의 상부와 하부에 5 cm의 bubble column을 가진 bulb type bubble bioreactor (BU)에서 건물중은 41.92 g으로 가장 많이 증가하였으며, cylindric tube bioreactor (CT)에서 건물중이 38.55g으로 가장 낮게 나타났다. 이들 두 생물반응기의 초기 kLa 값은 BU 생물반응기에서 6.98 h$^{-1}$로 가장 높게 측정되었고, 반대로 bubble column이 없는 CT 생물반응기에서 5.25 h$^{-1}$으로 가장 낮게 측정되었다. 그러나 이와 같은 초기 kLa값의 차이는 부정근내의 이차대사산물인 ginsenoside의 함량에는 영향을 주지 않는 것으로 나타났다. 또한 BU 생물반응기에서 bubble column의 길이를 기존의 5 cm에서 10 cm로 연장시켰을 때, 초기 kLa값이 6.52 h$^{-1}$에서 7.80 h$^{-1}$로 증가하면서 인삼부정근의 생장을 42.13 g에서 50.30 g으로 약 16% 증가시킨 것으로 나타났다.

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피인용 문헌

  1. Effects of Aeration Rate and Sparger Type on Growth and Ginsenoside Accumulation in Bioreactor Cultures of Ginseng Adventitious Root(Panax ginseng C.A. Meyer) vol.32, pp.2, 2005, https://doi.org/10.5010/JPB.2005.32.2.111
  2. Tools for biotechnological production of useful phytochemicals from adventitious root cultures vol.15, pp.1, 2016, https://doi.org/10.1007/s11101-014-9391-z
  3. Ginsenosides: prospective for sustainable biotechnological production vol.98, pp.14, 2014, https://doi.org/10.1007/s00253-014-5801-9