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Effects of Various Bioreactors on Growth and Ginsenoside Accumulation in Ginseng Adventitious Root Cultures(Panax ginseng C.A. Meyer)

다양한 생물반응기 형태가 인삼(Panax ginseng C.A. Meyer) 부정근의 생장과 Ginsenoside 생산에 미치는 영향

  • 김윤수 (중앙대학교 인삼산업연구센터) ;
  • 한은주 (충북대학교 첨단원예기술개발연구센터) ;
  • 백기엽 (충북대학교 첨단원예기술개발연구센터)
  • Published : 2004.09.01

Abstract

The type of air lift bioreactor affected the root growth in ginseng adventitious root cultures. Among bioreactors used in this experiment, bulb type bubble bioreactor (BU) was the best to increase root growth (41.92 g dry weight). The kLa value representing the oxygen transfer capacity from medium to explants (6.98 h$^{-1}$ ) in BU with 5 cm bubble column was higher than other bioreactors. On the other hand, cylindric tube bioreactor (CT) without bubble column resulted in minimum root growth (38.55 g dry weight) and kLa value (5.25 h$^{-1}$ ). Furthermore, the root growth (50.30 g dry weight) in BU with 10 cm bubble column more increased than 5 cm bubble column. However, the kLa value do not affected the secondary metabolite such as ginsenosides. These results show that the bubble column in air lift bioreactor increase kLa value and increased kLa value stimulate the growth of ginseng adventitious roots.

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  2. Tools for biotechnological production of useful phytochemicals from adventitious root cultures vol.15, pp.1, 2016, https://doi.org/10.1007/s11101-014-9391-z
  3. Ginsenosides: prospective for sustainable biotechnological production vol.98, pp.14, 2014, https://doi.org/10.1007/s00253-014-5801-9