Hypocholesterolemic Effect of Yogurt Supplemented Salicornia herbacea Extract in Cholesterol-Fed Rats.

콜레스테롤 급여 흰쥐에서 함초 요구르트의 콜레스테롤 저하효과

  • 차재영 ((주)바이오허브 부설연구소) ;
  • 전병삼 ((주)바이오허브 부설연구소) ;
  • 박정원 ((주)바이오허브 부설연구소) ;
  • 김범규 ((주)바이오허브 부설연구소) ;
  • 정찬영 (경상대학교 농업생명과학대학 응용생명과학부) ;
  • 류진수 (경상대학교 농업생명과학대학 응용생명과학부) ;
  • 최충국 (경상대학교 농업생명과학대학 응용생명과학부) ;
  • 조영수 (동아대학교 생명자원과학대학 응용생명공학부)
  • Published : 2004.10.01


The effect of yogurt containing Hamcho (Salicornia herbacea) extract (Hamcho yogurt: HY) on the lipid concentrations in male Sprague-Dawley rats fed a cholesterol diet were studied. Rats were assigned three groups in the experiment; cholesterol diet (the CHOL group), cholesterol diet plus yogurt by lactic acid bacteria (the Y group) or cholesterol diet plus yogurt supplemented Hamcho extract by lactic acid bacteria (the HY group). Fermented milk with or without Hamcho extract in the diet supplemented at the levels 5.0% for 3 weeks. The concentrations of total cholesterol and bile acid in serum was significantly lower in the HY group than the CHOL group. This effect was also observed in rats fed a diet supplemented fermented milk. This cholesterol-lowering effect was more pronounced in the HY group compared to the Y group. The atherogenic index was significantly lower in the HY or Y groups than the CHOL group. The concentration of non esterified fatty acid in serum was significantly lower in the HY group than the CHOL and the Y groups. These results suggested that Hamcho yogurt exert the hypocholesterolemic effect in cholesterol fed rats.

콜레스테롤 식이로 유발시킨 고콜레스테롤혈증 흰쥐에 유산균 발효유(요구르트) 및 해양식물인 함초(Salicornia herbacea) 추출물 첨가 유산균 발효유(함초 요구르트)를 식이중에 5% (w/w)수준으로 첨가하여 지질농도에 미치는 영향을 검토하였다. 혈청 총 콜레스테롤 농도는 콜레스테롤 식이군에 비해 함초 요구르트 및 요구르트 식이군에서 각각 유의적으로 감소하였다. 동맥경화 지수(atherogenic index)도 콜레스테롤 식이군에 비해 함초 요구르트 및 요구르트 식이군에서 각각 유의적으로 감소하여 항동맥경화 작용이 있는 것으로 나타났다. 함초 요구르트에 의한 콜레스테롤 감소 효과는 콜레스테롤로부터 담즙산 합성 증가에 의한 혈청 중의 담즙산 농도 증가와 장내 흡수 저해에 의한 분변 중으로의 증가에 의한 것으로 사료되었다. 혈중 유리 지방산 농도는 함초 요구르트 식이군에서 유의적으로 감소하였다. 혈당치는 함초 요구르트 식이에서만 유의적으로 감소되는 것으로 나타났다. 이상의 실험 결과로부터 함초 추출물 첨가 요구르트는 혈청 콜레스테롤 감소에 의한 항동맥경화 효과가 있는 것으로 나타나 해양식물 유래 신기능성 식품 개발 가능성이 대두되었다.



  1. Agerbaek, M., L. U. Gerdes and B. Richelsen. 1995. Hypocholesterolemic effect of a new fermented milk product in healthy middle-aged men. Eur. J. Clin. Nutr. 49, 346-352
  2. Anderson, J. W. and S. E. Gilliland. 1999. Effect of fermented milk (yogurt) containing Lactobacillus acidophilus L1 on serum cholesterol in hypercholesterolemic humans. J. Am. Coll. Nutr. 18, 43-50
  3. Annual report on the cause of death statistics. 2000. National Statistical Office, Republic of Korea
  4. Bang, M. A., H. A. Kim and Y. J. Cho. 2002. Hypoglycemic and antioxidant effect of dietary Hamcho powder in streptozotocin-induced diabetic rats. J. Korean Soc. Food Sci. Nutr. 31, 840-846
  5. Castelli, W. P., R. J. Garrison, P. W. F. Wilson, R. D. Abborr, S. Kalousdian and W. B. Kannel. 1986. Incidence of coronary heart disease and lipoprotein cholesterol levels. The Framingham study, JAMA, 256, 2835-2845
  6. Cha, J. Y., D. J. Kim and Y. S. Cho. 2000. Effect of chlorogenic acid on the concentrations of serum and hepatic lipid in rats. J. Korean Sco. Agric. Chem. Biotechnol. 43, 153-157
  7. Cha, J. Y., B. S. Jeon, G. G. Shin, D. W. Bae, H. K. Kim and B. K. Kim. 2002. The development of novel functional food as effective diabetic therapy on type 2 diabetes animals and patients (NIDDM). Nippon Nogeikagaku Kaisshi, Sendai, Japan, pp. 249
  8. Cha, J. Y., S. J. Jeong and Y. S. Cho. 2001. Effect of chicory root extract on cholesterol metabolism in rats. Agric. Chem. Biotechnol. 44, 131-134
  9. Cha, J. Y. and Y. S. Cho. 1999. Effect of potato polyphenolics on hyperlipidemia in rats. J. Korean Soc. Food Sci. Nutr. 29, 274-279
  10. Cha, J. Y. and Y. S. Cho. 2001. Effect of stem bark extract from Morus alba and Cudrania tricuspidata on the concentrations of lipid and tissue lipid peroxidation in the cholesterol-fed rats. Korean J. Food Sci. Technol. 33, 128-134
  11. Cha, J. Y., S. S. Hong, Y. S. Cho and D. J. Kim. 2003. Effects of different dietary fats and fibers on the lipid concentrations of liver and serum and biochemical index in rats. J. Korean Soc. Food Sci. Nutr. 32, 1377-1384
  12. Cho, Y. C., J. H. Ahn, S. M. Jeon, K. S. Lee and D. S. Kang. 2002. The study on phamarchological action of Hamcho (Salicornia herbacea). Korean J. Medicinal Crop Sci. 10, 93-99
  13. Cho, K. J., Y. S. Lee and B. H. Ryu. 1990. Antitumor effect and immunology activity seaweeds toward sarcoma-180. J. Korean Fish Soc. 23, 345-352
  14. Duncan, D.B. 1957. Mutiple range test for correlated and heteroscedastic means. Biometrics 13, 164-176
  15. Gilliand, S. E., C. R. Nelson and C. Maxwell. 1985. Assimilation of cholesterol by Lactobacillus acidoplilis. Appli. Environ. Microbiol. 49, 377-381
  16. Gordon, T., W. P. Casfelli, M. C. Hjortland, W. B. Kennel and T. R. Dawher. 1977. Hight density lipoprotein as a protective factor against coronary heart diseases, the Framingham study. Am. J. Med. 62, 707-714
  17. Grunewald, K. K. 1982. Serum cholesterol levels in rats fed skim milk fermented by Lactobacillus acidophilus. J. Food Sci. 47, 2078-2079
  18. Han, S. K., M. S. Kim and B. S. Pyo. 2003. Antioxidative effect of glasswort (Salicornia herbacea L.) on the lipid oxidation of pork. Korean J. Food Sci. Ani. Resour. 23, 46-49
  19. Han, S. K. and M. S. Kim. 2003. Antioxidative effect of Salicornia herbacea L. grown in closed sea beach. J. Korean Soc. Food Sci. Nutr. 32, 207-210
  20. Hepner, G., R. Fried, S. S. Jeor, L. Fusetti and R. Morin. 1979. Hypocholesterolemic effect of yoghurt and milk. Am. J. Clin. Nutr. 32, 19-24
  21. Ihm, B. S. and J. S. Lee. 1986. The strategies of Salicornia herbacea and Suaeda japonica for coping with environmental fluctuation of salt marsh. Korean J. Environ. Biol. 4, 15-25
  22. Jeong, C. Y., J. S. Ryu, C. K. Choi, B. S. Jeon, J. W. Park, G. G. Shin, B. K. Kim, D. W. Bae and J. Y. Cha. 2004. Preparation and Quality Characteristics of Fermented Milk Supplemented with Salicornia herbacea Extract Powder. Korean J. Life Sci. (submitted)
  23. Jeong, E. J. and B. H. Bang. 2003. The effect on the quality of yogurt added water extracted from sea tangle. Korean J. Food Nutr. 16, 66-71
  24. Jo, Y. C., J. H. Ahn, S. M. Chon, K. S. Lee, T. J. Bea and D. S. Kang. 2002. Studies on pharmacological effects of glasswort (Salicornia herbacea L.). Korean J. Medicinal Corp Sci. 10, 93-99
  25. Kim, M. H. 2000. The water-soluble extract of chicory reduces cholesterol uptake in gut-perfused rats. Nutr. Res. 20, 1017-1026
  26. Kim. D. S. and C. H. Kim. 2001. Effect of sea tangle, Laminaria japonicus, extract on activities of glucokinase and hexokinase in alloxan-induced diabetic mellitus mice. Korean J. Life Sci. 11, 467-482
  27. Lee, E. J. and M. K. Sung. 2001. Effect of fiber-rich sea mustard feeding on AOM-induced colon aberrant crypt formation and colonic cell proliferation in Sprague Dawley rats. J. Korean Soc. Food Sci. Nutr. 30, 535-539
  28. Lee, H. S., M. S. Choi, Y. K. Lee, S. H. Park and Y. J. Kim. 1996. A study on the development of high-fiber supplements for the diabetic patients (2)-Effect of seaweed supplementation on the lipid and glucose metabolism in streptozotocin-induced diabetic rats. Korean J. Nutr. 29, 296-306
  29. Lee, K. S., J. S. Seo and Y. S. Choi. 1998. Effect of sea tangle and hypoglycemic agent on lipid metabolism in diabetic rats. J. Korean Soc. Food Sci. Nutr. 27, 960-967
  30. Mann, G. V. and A. Spoerry. 1974. Studies of a surfactant and cholesterolemia in the Maasai. Am. J. Clin. Nutr. 27, 464-469
  31. Park, C. K., J. Y. Cha, B. S. Jeon, N. M. Kim and K. H. Shim. 2000. Effects of chicory root water extracts on serum triglyceride and microsomal triglyceride transfer protein (MTP) activity in rats. J. Koean Soc. Food Sci. Nutr. 29, 518-524

Cited by

  1. A salt substitute with low sodium content from plant aqueous extracts vol.44, pp.2, 2011,
  2. Physicochemical Characteristics and Physiological Activities of Naturally Fermented Glasswort (Salicornia herbacea L.) Juice vol.40, pp.11, 2011,
  3. Ameliorative effect of saltwort (Salicornia herbacea) extract on hepatic dysfunction and hyperlipidemia in rats vol.21, pp.2, 2012,
  4. Anti-obesity effect of Korean Hamcho (Salicornia herbaceaL.) powder on high-fat diet-induced obese rats vol.48, pp.2, 2015,
  5. Quality Characteristics of Yulmoo Mul-kimchi Containing Saltwort (Salicornia herbacea L.) vol.40, pp.7, 2011,
  6. Effect of Dietary Intake of Salicornia herbacea L. Hot Water Extract on Anti-obesity in Diet-induced Obese Rats vol.41, pp.7, 2012,