Protective Effects of Jihwangeumja on Oxidative Stress-induced Injury of Human Umbilical Vein Endothelial Cells

혈관내피세포의 산화적 손상에 대한 지황음자의 방어기전 연구

  • 정용준 (원광대학교 한의학전문대학원) ;
  • 장재호 (원광대학교 한의학전문대학원) ;
  • 이대용 (원광대학교 한의학전문대학원) ;
  • 이민구 (원광대학교 한의학전문대학원) ;
  • 전인철 (원광대학교 한의학전문대학원) ;
  • 정대영 (원광대학교 심계내과학교실) ;
  • 이인 (원광대학교 심계내과학교실) ;
  • 신선호 (원광대학교 심계내과학교실) ;
  • 문병순 (원광대학교 한의학전문대학원)
  • Published : 2004.06.01

Abstract

Objectives : Oxidative stress can induce negative responses such as growth inhibition or cell death by necrosis or apoptosis due to the intensity of the oxidative stress, as well as positive responses such as cellular proliferation or activation. We examined the effect of Jihwangeumja on this process. Methods and Results : We analyzed the influence of oxidative stress and agents that modify its effect in human umbilical vein endothelial cell (HUVEC). Oxidative stress was induced by $B_2O_2$. With induced oxidative stress the results obtained indicate that it has a harmful effect over cell function and viability, and that this effect is dose and time dependent. When oxidative stress increased, Jihwangeumja reduced cell damage and had protective functions. $B_2O_2$, induced the apoptosis of HUVEC through the activation of intrinsic caspases pathway as well as mitochondrial dysfunction. A significant increase in cell survival was observed in culture cells with oxidative stress when they were treated with Jihwangeumja. Conclusions : These results suggest that Jihwangeumja may be potentially useful to treat HUVEC against oxidative damages mediated by modulation of caspase protease and mitochondrial dysfunction.

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