Protective Effects of Jihwangeumja on Oxidative Stress-induced Injury of Human Umbilical Vein Endothelial Cells

혈관내피세포의 산화적 손상에 대한 지황음자의 방어기전 연구

  • 정용준 (원광대학교 한의학전문대학원) ;
  • 장재호 (원광대학교 한의학전문대학원) ;
  • 이대용 (원광대학교 한의학전문대학원) ;
  • 이민구 (원광대학교 한의학전문대학원) ;
  • 전인철 (원광대학교 한의학전문대학원) ;
  • 정대영 (원광대학교 심계내과학교실) ;
  • 이인 (원광대학교 심계내과학교실) ;
  • 신선호 (원광대학교 심계내과학교실) ;
  • 문병순 (원광대학교 한의학전문대학원)
  • Published : 2004.06.01


Objectives : Oxidative stress can induce negative responses such as growth inhibition or cell death by necrosis or apoptosis due to the intensity of the oxidative stress, as well as positive responses such as cellular proliferation or activation. We examined the effect of Jihwangeumja on this process. Methods and Results : We analyzed the influence of oxidative stress and agents that modify its effect in human umbilical vein endothelial cell (HUVEC). Oxidative stress was induced by $B_2O_2$. With induced oxidative stress the results obtained indicate that it has a harmful effect over cell function and viability, and that this effect is dose and time dependent. When oxidative stress increased, Jihwangeumja reduced cell damage and had protective functions. $B_2O_2$, induced the apoptosis of HUVEC through the activation of intrinsic caspases pathway as well as mitochondrial dysfunction. A significant increase in cell survival was observed in culture cells with oxidative stress when they were treated with Jihwangeumja. Conclusions : These results suggest that Jihwangeumja may be potentially useful to treat HUVEC against oxidative damages mediated by modulation of caspase protease and mitochondrial dysfunction.


  1. 中國處方大辭典 팽부인
  2. Arch. Biochem. Biophys. v.394 no.1 Hypochlorous acid stimulation of the mitogen-activated protein kinase pathway enhances cell survival Midwinter R.G.;Vissers M.C.;Winterbourn C.C.
  3. Zhonghua Shao Shang Za Zhi v.17 no.6 The role of released cytochrome c from mitochondria in the apoptosis of HUVECs induced by hydrogen peroxide Yuan J.;Cheng J.;Qin X
  4. Biochem. J. v.365 no.Pt3 Activated neutrophils oxidase extracellular proteins of endothelial cells in culture : effect of nitric oxide donors Czapski G.A.;Avram D.;Sakharov D.V.;Wirtz K.W.;Strosznajder J.B.;Pap E.H.
  5. Prostaglandins Other Lipid Mediat. v.67 no.1 Effects of oxidative stress and antioxidant treatments on eicosanoid synthesis and lipid peroxidation in long term human umbilical vein endothelial cells culture Estrada-Garcia L.;Carrera-Rotllan J.;Puig-Parellada P
  6. 四物湯이 血管內皮細胞에 미치는 影響 南昌圭
  7. 병리학 대한병리학회
  8. HARRISON'S 내과학 해리슨 번역 편찬위원회
  9. 宣明論方(醫方類聚券五) 劉河間
  10. 동양의학대사전 전통의학연구소
  11. 證治準繩(券一) 王肯堂
  12. 證脈 方藥合編 黃度淵
  13. 東醫寶鑑 許浚
  14. 東醫方劑와 處方解說 尹用甲
  15. 한방임상병리학 鄭遇悅;安圭錫
  16. Free Radic Biol. Med. v.30 no.6 Variants of peroxiredoxins expression in response to hydroperoxide stress Mitsumoto A.;Takanezawa Y.;Okawa K.;Iwamatsu A.;Nakagawa Y
  17. 노화 김숙희;김화영
  18. 노인의학 의학교육연수원
  19. 張氏醫通 장로
  20. 한의학기초이론 羅昌洙;孟雄在;蔡禹錫
  21. 四物湯이 老化白鼠 腦組織의 生化學的 變化에 미치는 影響 朴鍾雲
  22. 內經靈樞解析 楊維傑
  23. 黃帝內經 洪元植(편저)
  24. Science v.281 no.5381 Mitochondria and apoptosis Green D.R.;Reed J.C.
  25. Biol. Signals Recept. v.10 no.3-4 Mitochondria and apoptosis Gottlieb R.A.
  26. Zhonghua Shao Shang Za Zhi v.17 no.6 The role of released cytochrome c from mitochondria in the apoptosis of HUVECs induced by hydrogen peroxide Yuan J.;Cheng J.;Qin X.
  27. J. Exp. Med. v.191 no.1 Ionizing radiation and chemotherapeutic drugs induce apoptosis in lymphocytes in the absence of Fas or FADD/MORT1 signaling. Implications for cancer therapy Newton K.;Strasser A.