Pulsatilla Saponin D: the Antitumor Principle from Pulsatilla koreana

  • Published : 2004.09.01

Abstract

By bioassay-guided separation, an already known saponin, Pulsatilla saponin D was isolated from the root of Pulsatilla koreana Nakai as a antitumor component when evaluated by in vivo antitumor activity as well as in vitro cytotoxic activity test. It showed potent inhibition rate of tumor growth (IR, 82%) at the dose of 6.4 mg/kg on the BDF1 mice bearing LLC cells.

References

  1. Bae, K. H., The medicinal plants of Korea. Kyo-Hak Press, 139 (1999)
  2. Campbell, W. E., Cragg, G. M. L., and Powrie, A. H., Anemonin, protoanemonin and ranunculin from Knowltonia capensis. Phytochemistry, 18, 323-324 (1979) https://doi.org/10.1016/0031-9422(79)80083-7
  3. Kang, S. S., Saponins from the root of Pulsatilla koreana. Arch. Pharm. Res., 12, 42-47 (1989) https://doi.org/10.1007/BF02855745
  4. Kim, Y., Kim, S. B., You, Y. J., and Ahn, B. Z., Deoxypodophyllotoxin; The cytotoxic and antiangiogenic component from Pulsatilla koreana. Planta Med., 68, 268-271 (2002) https://doi.org/10.1055/s-2002-23143
  5. Kim, Y., You, Y., J., Bang, S. C., Lee, C. W., Kim, H. M., and Ahn, B. Z., In vivo, antitumor activity of $SB31^{\circledR}$ injection. Phytother. Res. (2004, submitted)
  6. Kim, S. Y. and Kim, S. B., Anti-tumor effects of extracts of Pulsatilla koreana ($SB-31^{\circledR}$) in vitro. J. Kor. Cancer Assoc., 26 (6), 959-963 (1994)
  7. Li, R. Z. and Ji, X. J., The cytotoxicity and action mechanism of Ranunculin in vitro. Yao Hsueh Hsueh Pao, 28, 326-331 (1993)
  8. Martin, M. L., San Roman, L., and Dominguez, A., In vitro activity of protoanemonin, an antifungal agent. Planta Med., 56, 66-69 (1990) https://doi.org/10.1055/s-2006-960886
  9. Skehan P, Storeng R., Scudiero D., Monks A., McMahon J., Vistica D., Warren J. T., Bokesh H., Kenney S., and Boyd M. R., Feasibility of a highly-flux anticancer drug screen using a diverse panel of cultured human tumor cell lines. J. Natl. Cancer Inst., 82, 1107-1112 (1990) https://doi.org/10.1093/jnci/82.13.1107
  10. Teruhiro U, Jiro S, Yoshikazu S, Kumio A, and Yuji Y, Antitumor activity of a novel podophylotoxin derivatives (TOP53) against lung cancer and lung matastatic cancer. Cancer Res., 56, 2809-2814 (1996)
  11. Viqar U. A., Spectroscopic data of saponins. CRC Press, 1469 (2000)
  12. Ye, W. C., Ou, B. X., Ji, N. N., Zhao, S. X., Ye, T., Anthony, M. K., and Paul, S., Patensin, a saponin from Pulsatilla patens var. multifida. Phytochemistry, 39 (4), 937-939 (1995) https://doi.org/10.1016/0031-9422(95)00021-X
  13. Yoshihiro M., Akihito Y., Minpei K., Tomoki A., and Yutaka S., Triterpene saponins and lignans from the root Pulsatilla chinensis and their cytotoxic activity against HL-60 cells. J. Nat. Prod., 62, 1279-1283 (1999) https://doi.org/10.1021/np9901837