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Eletrochemical Detection of Gene using Microelectrode-array DNA Chip

미소전극어레이형 DNA칩을 이용한 유전자의 전기화학적 검출

  • ;
  • ;
  • Eiichi Tamiya (Japan Advanced Institute of Science and Technology, School of Materials Scienc) ;
  • 최용성 (원광대학교 전기전자 및 정보공학부) ;
  • 권영수 (동아대학교 전기전자 및 컴퓨터공학) ;
  • ;
  • 박대희 (원광대학교 전기전자 및 정보공학부)
  • Published : 2004.07.01

Abstract

In this paper, a DNA chip with a microelectrode array was fabricated using microfabrication technology. Several probe DNAs consisting of mercaptohexyl moiety at their 5 end were immobilized on the gold electrodes by DNA arrayer. Then target DNAs were hybridized and reacted with Hoechst 33258, which is a DNA minor groove binder and electrochemically active dye. Linear sweep voltammetry or cyclic voltammetry showed a difference between target DNA and control DNA in the anodic peak current values. It was derived from Hoechst 33258 concentrated at the electrode surface through association with formed hybrid. It suggested that this DNA chip could recognize the sequence specific genes.

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