Biocontrol of Cabbage Clubroot by the Organic Fertilizer Using Streptomyces sp. AC-3.

Streptomyces sp. AC-3을 이용한 배추 무사마귀병의 생물학적 방제

  • Published : 2004.06.01

Abstract

This research is performed for a biological control of Chinese cabbage clubroot, we isolated an antagonistic bacterium AC-3 against Plasmodiophora sp., causal pathogens of cabbage clubroot. The isolated strain was identified as Streptomyces sp. by culture morphology, biochemical reactions, and homology research based on l6S rDNA sequences. Streptomyces sp. AC-3 produced chitinase (9.3 units/$m\ell$) in culture broth. So Plasmodiophora sp. mycelia changed abnonnal swelling, curling and branching mycelia by Streptomyces sp. AC-3 culture. In a field infected by Plasmodiophora sp., the treatment of a organic fertilizer added 2% Streptomyces sp. AC-3 microbial inoculant, it resulted in about 50% reducing the severity of cabbage clubroot significantly on cabbage plants compared with treated organic fertilizer plants. Additional disease such as sclerotinia rot, fusarium wilt and pythium rot were also significantly reduced by the treatment of the organic fertilizer added Streptomyces sp. AC-3 microbial inoculant.

References

  1. J. Ferm. Bioeng. v.75 Production of the antifungal peptide antibiotic, iturin by Bacillus subtilis NB22 in solid state fermentaion Akihiro, O.;A. Takashi;S. Makoto https://doi.org/10.1016/0922-338X(93)90172-5
  2. Aust. J. Biol. Sci. v.24 Bacteria and actinomycetes antagonistic to fungal root pathogens in Australian soils Broadbent, P.;K. F. Barker;Y. Waterworth https://doi.org/10.1071/BI9710925
  3. J. Indust. Microbiol. Biotechnol. v.23 In vitro and in vivo antagonism of pathogenic turfgrass fungi by Streptomyces hygroscopicus strain YCED9 and WYE53 Chamberlain, K.;D. L. Crawford https://doi.org/10.1038/sj.jim.2900671
  4. Enzymol. Microb. Technol. v.6 Recent advances in studies of the mechanisms of microbial degradation of lignins Crawford, R. L.;D. L. Crawford https://doi.org/10.1016/0141-0229(84)90092-9
  5. Can. J. Bot. v.71 Inhibitory effects of UV mutants of Streptomyces corchorusii and Streptomyces spiroverticillium on bean and banana wilt pathogens El-Abyad, M. S.;M. A. El-Sayed;A. R. El-Shanshoury;N. H. El-Batanouny https://doi.org/10.1139/b93-125
  6. Can. J. Microbiol. v.28 Degradation of plant pathogenic fungi by Trichoderma harzianum Elad, Y.;I. Chet;Y. Henis https://doi.org/10.1139/m82-110
  7. Annu. Rev. Microbiol. v.37 Ecology of actinomycetes Goodfellow, M.;S. T. Williams
  8. Poster presentation No. P-1-⑦ Control effect of chinese cabbage clubroot (Plasmodiophora brassicae) disease by beneficial antagonist Kim, J. H.;Y. R. Yeoung;B. S. Kim;J. Y. Jeon
  9. Bergey's Manual of Systematic Bacteriology The Actinomycetes: A Practical Guide to Genetic Identification of Actinomycetes Lechevalier, H. A.;S. T. Williams;M. E. Sharpe;J. G. Holt
  10. Appl. Environ. Microbiol. v.57 Pseudomonas stutzeri YPL-1 genetic transformation and antifungal mechanism against Fusarium solani, an agent to plant root rot Lim, S. H.;K. S. Kim;S. D. Kim
  11. Mol. Plant Pathol. v.83 Antifungal synergistic interaction between chitinlytic enzymes from Trichoderma harzanum and Enterobacter cloacae Lorito, M.;A. Di Pietro;C. K. Hayer;S. L. Woo;G. E. Harman
  12. Microbiol. v.141 Nucleotide sequences of streptomycete 16S ribosomal DNA: Towards a specific identification system for streptomycetes using PCR Mehling, A.;F. Wehmeir;W. Piepersberg https://doi.org/10.1099/13500872-141-9-2139
  13. Anal. Chem. v.31 Use of dinitrosalicylic acid reagent for determination of reducing sugar Miller, G. L. https://doi.org/10.1021/ac60147a030
  14. Can. J. Microbiol. v.9 Lysis of soil fungi by bacteria Mitchell, R.;M. Alexander https://doi.org/10.1139/m63-023
  15. Kor. J. Microbiol. Biotechnol. v.30 Chitinase production and isolation of Serratia plymuthica AL-1 antagonistic to white rot fungi from Allium fistulosum roots Joo, G. J.;I. H. Lee;J. H. Kim
  16. Kor. J. Life Sci. v.12 Isolation and antifungal activity of Bacillis ehimensis strain YJ-37 as antagonistic against vegetables damping-off fungi Joo, G. J.;J. H. Kim;S. J. Kang https://doi.org/10.5352/JLS.2002.12.2.200
  17. Methods of soil analysis, part 3. Chemical methods, Agronomy, Book series number 5 Sparks, D. L.
  18. World J. Microbiol. Biotechnol. v.7 Physiology and nutritional aspects of actinomycetes: An overview Srinivasan, M. C.;R. S. Laxman;M. V. Deshpande https://doi.org/10.1007/BF00328987
  19. Soil & Tillage Research v.41 A review of plant disease, pathogen interactions and microbial antagonism under conservation tillage in temperate humid agriculture Sturz, A. V.;M. R. Carter;H. W. Johnston https://doi.org/10.1016/S0167-1987(96)01095-1
  20. J. Agric. Sci. Finl. v.59 The biological control of seedborne Alternaria brassicicola of cruciferous plants with a powdery preparation of Streptomyces sp. Tahvonen, R.;H. Avikainen
  21. An. Agric. Fenniae v.27 Biological control of Botrytis cinerea and Rhizoctonia solani in lettuce by Streptomyces sp. Tahvonen, R.;L. Lahdenpera
  22. Plant - Microbe interactions Current concepts in the use of introduced bacteria for biological disease control: mechanisms and antifungal metabolites Thomashow, L. S.;D. M. Weller;G. Stacey(ed.);N. Keen(ed.)
  23. Annu. Rev. Phytopathol. v.36 Systemic resistance induced by rhizosphere bacteria van Loon, L. C.;P. A. H. M. Bakker;C. M. J. Pieterse https://doi.org/10.1146/annurev.phyto.36.1.453
  24. J. Agric. Res. v.51 Calcium cyanamide in relation to control of clubroot of cabbage Walker, J. C.
  25. Appl. Environ. Microbiol. v.63 Purification and characterization of two bifunctional chitinase/lysozyme extracellularly produced by Pseudomonas aeruginosa K-187 in a shrimp and crab shell powder medium Wnag, S. L.;W. T. Chang
  26. Mycological Research v.95 Boron, pH and inoculum concentration influencing colonization by Plasmodiophora brassicae Webster, M. A.;G. R. Dixon https://doi.org/10.1016/S0953-7562(09)81363-4
  27. Agronomy J. v.88 Utilization of allelopathy for weed management in agroecosystems Weston L. A. https://doi.org/10.2134/agronj1996.00021962003600060004x
  28. Soil Biol. and Biochem. v.23 Phenolic compounds in conductive and suppressive soils on clubroot disease of crucifers Young, C. C.;K. T. Cheng;G. R. Waller https://doi.org/10.1016/0038-0717(91)90032-F