The Effect of Low-Intensity Treadmill Exercise on Blood Levels of Glucose, Insulin and Lipids in Streptozotocin-Induced Diabetic Rats

저강도 treadmill 운동이 streptozotocin 유도 당뇨쥐의 혈당, 인슐린 및 지질 농도에 미치는 영향

  • 김세종 (부산대학교 체육학과) ;
  • 서혜림 (부산대학교 체육학과) ;
  • 고정림 (부산대학교 체육학과) ;
  • 염종우 (부산대학교 체육학과) ;
  • 예정복 (부산대학교 체육학과) ;
  • 이선주 (부산대학교 나노기술학과) ;
  • 김경환 (부산대학교 나노기술학과) ;
  • 손원협 (부산대학교 나노기술학과) ;
  • 장은숙 (부산대학교 나노기술학과)
  • Published : 2004.04.01


Exercise is beneficial to the diabetic patients and streptozotocin-induced diabetic rat has been used for the study of exercise effect. The purpose of this study was to establish the optimal condition of induction of hyperglycemic diabetic rat using streptozotocin and to examine the preventive effect of treadmill exercise on the diabetic rat before and after streptozotocin injection. Intraperitoneal injection of increasing amount of streptozotocin up to 40 mg/kg dose-responsively induce hyperglycemic diabetic rat and inversely reduced the blood insulin level. Body weight was also gradually reduced with the increasing amount of streptozotocin. Control and diabetic rats exercised for 4 weeks before streptozotocin injection. The exercise was performed in the treadmill for 25 minutes a day and 5 times a week with low intensity (0 degree tilt, 15 m/min velocity). Following streptozotocin injection, the blood glucose level was measured every week and the rat was sacrificed after 4 weeks to measure the concentration of insulin and blood lipids. The blood levels of glucose and insulin was significantly reduced with exercise before streptozotocin injection, while those were not changed after streptozotocin injection. The levels of blood lipids such as total cholesterol, HDL-cholesterol, LDL-cholesterol and triglyceride were close to normal control rats. From this study, researchers found the optimal condition of preparation of streptozotocin-induced hyperglycemic diabetic rat, and the mild treadmill exercise has beneficial effect on preventing hyperglycemia and hyperlipidemia. Thus, even low intensive running prevent not only diabetes but also diabetic vascular complications.


  1. Mehods Find Exp Clin Pharmacol v.25 no.3 Protective effect of pioglitazone against multiple low-dose streptozotocin-induced diabetes in rats Anjaneyulu,M.;P.Ramarao
  2. Trends Endocrinol. Metab. v.8 Autoimmunity and type I diabetes Bach,J.F.
  3. Experientia v.52 N-mono-methyl-arginine and nicotinamide prevent streptozotocin-induced double strand DNA break formation in pancreatic rat islets Bedoya,F.J.;F.Solano;M.Lucas
  4. Can. J. Appl. Physiol. v.20 Benefits of Exercise for Type Ⅱ Diabetics: Convergence of Epidemiologic, Physiologic,; and Molecular Evidence Bonen,A.
  5. Cell v.89 The role of fat in autoimmune diabetes Chervonsky,A.V.;Y.Wang;F.S.Wong;I.Visintin;R.A.Flavell;C.A.Janeway;L.A.Matis
  6. Diabetes v.32 Glucose turnover during recovery from intensive exercise Cualies,J.;J.J.Cunningham;L.Nelson;N.Btown;E.Nadel;R.S.Sherwin
  7. Current Therapy of Diabetes Mellitus (first edition) DeFronzo,R.A.
  8. Biochem Pharmacol v.50 Comparison of inhibition of glucose stimulated insulin secretion in rat islets of Langerhans by streptozotocin and methyl and ethyl nitrosoureas and methanesulphonates. Lack of correlation with nitric oxide-releasing or $O^6$-alkylating ability Delaney,C.A.;A.Dunger;M.Di Matteo;J.M.Cunningham;M.H.Green;I.C.Green
  9. N. Engl. J. Med. v.303 Cytotoxic autoantibodies to beta cells in the serum of patients with insulin-dependent diabetes mellitus Doberson,M.J.;J.E.Scharff,;F.Ginsberg-Fellner;A.L.Notkins
  10. N. Engl. J. Med. v.314 Type I diabetes mellitus : A chronic autoimmune disease Eisenbarth,G.S.
  11. Diabetes v.25 Relative importance of transport and alkylation for pancreatic beta-cell toxicity of streptozotocin Elsner,M.;B.Guldbakke;M.Tiedge;R.Munday;S.Lenzen
  12. Diabetologia v.40 Exercise and the metabolic syndrome Eriksson,J.;S.Taimela;V.A.Koivisto
  13. J. Nutr v.131 no.9 Exercise down-regulates hepatic fatty acid synthase in streptozotocin treated rats Fiebig,R.G.;J.M.Hollander;L.L.Ji
  14. Annu. Rev. Med. v.49 Exercise, glucose transport and insulin sensitivity Goodyear,L.J.;B.B.Kahn
  15. Am. J. Physiol. v.273 Exercise regulation of glucose transport in skeletal muscle Hayashi,T.;J.F.P.Wojtaszewski;L.J.Goodyear
  16. Sports Med. v.24 Role of exercise training in the prevention and treatment of insulin resistance and non-insulin-dependent diabetes mellitus Ivy,J.L.
  17. J. Basic Clin Physiol Pharmacol. v.6 no.3-4 The effect of metformin on insulin receptors and lipid perosidation in alloxan and streptozotocin inducel diabetes Kanigur,S.G.;M.Guven;I.Onaran;V.Tezcan;A.Cenani;H.Hatemi
  18. Biol Chem Hoppe-Seyler v.376 Nitric oxide generation during cellular metabolization of the diabetogenic N-methyl-N-nitroso-urea streptozotocin contributes to islet cell DNA damage Kroncke,K.D.;K.F.Fehsel;A.Sommer;M.L.Rodriguez;V.Kolb-Bachofen
  19. Med. Sci. Sports Exe. v.23 no.6 Effects of exercise training on glucose control, lipid metabolism, and insulin sensitivity in hypertriglyceridemia and non-insulin dependent diabetes mellitus Lampman,R.M.;D.E.Schteingart
  20. Diabetologia v.28 Molecular biology of type I (insulin-dependent) diabetes mellitus Lernmark,A.
  21. Diabetologia v.20 A prospective analysis of antibodies reacting with pancreatic islet cells in insulin-dependent diabetic children Lernmark,A.;B.Haggloff;Z.Freedman;J.Irvine;J.Ludvigsson;G.Holmgren
  22. Food Technology and Nutrition v.Ⅱ Encyclopedia of Food Science, Macrae,R.;R.K.Robinson;M.J.Sadler
  23. Essentials of Human Nutrition Diabetes mellitus Mann,J.;Mann J.(ed.);Truswell AS(ed.)
  24. Pharmacol Res v.44 no.5 Improvement in insulin sensitivity by losartan in non-insulin dependent diabetic (NIDDM) rats Murali,B.;R.K.Goyal
  25. Endocr J v.49 no.5 Exercise training increases the activity of pyruvate dehydrogenase complex in skeletal muscle of diabetic rats Nakai,N.;Y.Miyazaki;Y.Sato;M.Nagasaki;M.Tanaka;K.Nakashima;Y.Shimomura
  26. Br. J. Sports Med. v.33 Diabetes and exercise Peirce,N.S.
  27. Metabolism v.49 no.11 A new rat model of type 2 diabetes: the fat-fed, streptozotocin treated rat Reed,M.J.;K.Meszaros;L.J.Entes;M.D.Claypool;J.G.Pinkett;T.M.Gadbois and G.M.Reaven
  28. Diabetes Care v.7 Improvement of glucose homeostasis after exercise training in non-insulin dependent diabetes Reitmmann,J.S.
  29. Diabetes v.43 STZ transport and cytotoxicity. Specific enhancement in GLUT2 expressing cells Schnedl,W.J.;S.Ferber;J.H.Johnson;C.B.Newgard
  30. Neurosci Lett v.346 no.3 Trendmill exercise suppresses diabetes-induced increment of nenropeptide Y expression in the gypothalamus of rats Shin,M.S.;Kim,H.;Chang,H.K.;Lee,T.H.;Jang,M.H.;Shin,M.C.;Lim,B.V.;Lee,H.H.;Kim,Y.P.;Yoon,J.H.;Jeong,I.G.;Kim,C.J.
  31. Physiol. Res. v.50 The mechanism of alloxan and streptozotocin action in β cells of the rat pancreas Szkudelski,T.
  32. Endocrinology v.138 Short-term insulin treatment prevents the diabetogenic action of streptozotocin in rats Thulesen,J.;C.Orskov;J.J.Holst;S.S.Poulsen
  33. West Indian Med. J. v.45 Streptozotocin alters pancreatic beta-cell responsiveness to glucose within six hours of injection into rats West,E.;O.R.Simon;E.Y.Morrison
  34. Am. J. Physiol. v.277 AMP-activated protein kinase, a metabolic master switch: possible roles in Type 2 diabetes Winder,W.W.;D.G.Hardie
  35. Am J Physiol Endocrinol Metab v.282 Physical exercise prevents the development of type 2 diabetes mellitus in Psammomys obesus Yuval,H.;S.Yair;S.Yoav;S.M.Dani;L.Lea;B.Liora;R.S.Sanford;M.Joseph
  36. The medical clinics of North America v.69 Diabetes and exercise Zinmann,B.;M.Vranic