Isolation and Characterization of Chlorothalonil-dissipating Bacteria from Soil.

토양으로부터 Chlorothalonil 전환 미생물의 분리 및 특성

  • 이수현 (경북대학교 농화학과) ;
  • 신재호 (경북대학교 농화학과) ;
  • 최준호 (경북대학교 농화학과) ;
  • 박종우 (경북대학교 농화학과) ;
  • 김장억 (경북대학교 농화학과) ;
  • 이인구 (경북대학교 농화학과)
  • Published : 2004.03.01


Chlorothalonil is a wide-spectrum fungicide that is widely used in the world. Chlorothalonil is known as a potential toxic pollutant due to its high application rate, persistence, and toxicity to humans and other species. With the Increase of necessity of bioremediation, this study was conducted to isolate the chlorothalonil dissipation bacteria from soil. Soil samples were collected from 184 sites of farmland and wastewater disposal soil.661 strains resistant to chlorothalonil were isolated by dilution method from chlorothalonil-containing enrichment culture. After incubating at $30^{\circ}C$ in 1/10 LB media containing 10 ppm of chlorothalonil for a week, dissipation ability of chlorothalonil was investigated by HPLC. Finally, a strain SH35B, capable of dissipating chlorothalonil efficiently, was selected. The strain SH35B was identified as Ochrobactrum sp. Ten ppm of chlorothalonil In 1/10 LB media were completely dissipated by the growth of Ochrobactrum sp. SH35B for 30 h at $30^{\circ}C$. In the isolated strain, the content of glutathione and the activity of glutathione S-transferase were supposed to be ones of the Important factors for chlorothalonil dissipation and were higher than those of control strains, Escherichia coli and Bacillus subtilis.


Chlorothalonil;Ochrobactrum sp.;dissipation


  1. Appl. Environ. Microbiol. v.66 16S rRNA gene-based detection of tetrachloroethene dechlorinating desulfuromonas and dehalococcoides species Frank,E.L.;S.Qing;L.Jieran;M.T.James
  2. Meth. Enzmol v.77 Glutathione S-transferase(rat and human) Habig,W.H;W.B.Jakoby
  3. Arch. Environ. Contam. Toxicol. v.40 Transformation pathways of $^{14}C$-chlorothalonil in tropical soils Regitano,J.B.;V.L.Tornisielo;A.Lavorenti;R.S.Pacovsky
  4. Mol. Gen. Genet. v.152 Restriction and modification in Bacillus species. Genetic transformation of bacteria with DNA from different species. part I. Uozumi,T.;T.Hoshino;K.Miwa;S.Horinouchi;T.Beppu;K.Arima
  5. J. Pestic. Sci. v.16 population change and characteristics of chlorothalonil degrading bacteria in soil Katayama,A.;H.Isemura;S.Kuwatsuka
  6. Pestic. Biochem. Physiol v.40 Soil transformation of alachlor via glutathione conjugation Paul,C.;C.Feng
  7. Anal. Biochem. v.190 Microtiter plate assay for the measurement of glutathione and glutathione disulfide in large numble of biological samples Baker,M.A.;G.J.Cerniglia;A.Zaman
  8. gairdeneri. Aquatic. Toxicol. v.7 The toxicology and metabolism of chlorothalonil in fish. i. lethal levels for Salmo gairdneri, Galaxias maculatus, G. truttaceous and G. auratus and the fate of $^C$-TCIN in S. gairdneri Davies,P.E.;R.W.G.White
  9. Appl. Environ. Microbiol. v.66 Enrichment of an endosulfan-degrading mixed bacterial culture Sutherland,T.D.;I.Horne;M.J.Lacey;R.L.Harcourt;R.J.Russell;J.G.Oakeshott
  10. Mol. Gen. Genet v.152 Restriction and modification in Bacillus species. Genetic transformation of bacteria with DNA from different specties. Part Ⅰ. Uozumi,T.;T.Hoshino;K.Miwa;S.Horinouchi;T.Beppu;K.Arima
  11. Molecular cloing, a laboratory manual.(3rd ed.) Sambrook,J.;D.W.Russell
  12. J. Biol. Chem. v.249 Glutathiones S-transferase : the first enzymatic steps in mercaturic acid formation Habig,W.H.;H.J.Pabst;W.B.Jakoby
  13. Biol. Fertil. Soils v.23 Biodegradation of chlorothalonil in soil after suppression of degradation Motonaga,K.;K.Tagagi;S.Matumoto
  14. Glutathione : Metabolism and function Arias,M.;W.B.Jakoby
  15. J. Mol. Biol v.166 Studies on transformation of Escherichia coli with plasmid Hanahan,D.
  16. Evniron. Tox. Chem. v.17 Suppression of chlorothalonil degradation in soil after repeated application Motonaga,K.;K.Tagagi;s.Matumoto
  17. Pestic. Sci. v.4 The synthesis and biological activity of substituted chlorinated benzenedicarbonitriles Clifford,D.P.;F.Edamura
  18. Fundam. Appl. Toxicol. v.33 Biotransformation of the fungicide chlorothalonil by glutathione conjugation Rosner,E.;C.Christa;W.Dekant
  19. J. Pest. Sci. v.22 Ubiquitous capability to substitute chlorine atoms of chlorothalonil in bacteria Katayama,A.;T.Itou;T.Ukai
  20. Metabolic pathway of agrochemicals. part 2: Insecticides and fungicides Robert,T.;D.Huston
  21. J. Pest. Reform v.17 Chlorothalonil Cox,C.