Isolation and Characterization of Chlorothalonil-dissipating Bacteria from Soil.

토양으로부터 Chlorothalonil 전환 미생물의 분리 및 특성

  • 이수현 (경북대학교 농화학과) ;
  • 신재호 (경북대학교 농화학과) ;
  • 최준호 (경북대학교 농화학과) ;
  • 박종우 (경북대학교 농화학과) ;
  • 김장억 (경북대학교 농화학과) ;
  • 이인구 (경북대학교 농화학과)
  • Published : 2004.03.01

Abstract

Chlorothalonil is a wide-spectrum fungicide that is widely used in the world. Chlorothalonil is known as a potential toxic pollutant due to its high application rate, persistence, and toxicity to humans and other species. With the Increase of necessity of bioremediation, this study was conducted to isolate the chlorothalonil dissipation bacteria from soil. Soil samples were collected from 184 sites of farmland and wastewater disposal soil.661 strains resistant to chlorothalonil were isolated by dilution method from chlorothalonil-containing enrichment culture. After incubating at $30^{\circ}C$ in 1/10 LB media containing 10 ppm of chlorothalonil for a week, dissipation ability of chlorothalonil was investigated by HPLC. Finally, a strain SH35B, capable of dissipating chlorothalonil efficiently, was selected. The strain SH35B was identified as Ochrobactrum sp. Ten ppm of chlorothalonil In 1/10 LB media were completely dissipated by the growth of Ochrobactrum sp. SH35B for 30 h at $30^{\circ}C$. In the isolated strain, the content of glutathione and the activity of glutathione S-transferase were supposed to be ones of the Important factors for chlorothalonil dissipation and were higher than those of control strains, Escherichia coli and Bacillus subtilis.

Keywords

Chlorothalonil;Ochrobactrum sp.;dissipation

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