Diacylglycerol Production by Enzymatic Glycerolysis of Soybean Oil.

대두유에서 글리세롤리시스 반응을 이용한 디글리세리드의 효소적 생산

  • 박경준 (서울산업대학교 식품공학과) ;
  • 안은영 (서울산업대학교 식품공학과) ;
  • 권기석 (서울산업대학교 식품공학과) ;
  • 김강성 (용인대학교 식품영양학과) ;
  • 강성태 (서울산업대학교 식품공학과)
  • Published : 2004.03.01

Abstract

Diglyceride (DG) was prepared by reaction of soybean oil and glycerol in the presence of lipase. The initial rate of DG production was greatly affected by the amount of lipase. However the DG content at equilibrium was hardly affected by the amount of lipase added to the reaction mixture. The initial rate of FFA formation was highly affected by the moisture content between 0.5 and 2.3%, but at higher water content (3.3-5.2%), there was a small increase in the rate. And DG content at equilibrium slowly increased with the increase of the water content in glycerol up to 4.4%. However, there was a sharp decrease in DG content at higher water content (5.2-6.4%) due to higher free fatty acid production. The highest yield of DC was obtained at the temperature ranges of 30-5$0^{\circ}C$. The final yield of DG was not dependent on the glycerol (GL) to triglyceride (TG) molar ratio. However, at the molar ratio of 0.75:1 (GL/TG), the enzyme-catalyzed reaction was highly efficient and utilized all the glycerol. In optimized conditions for glycerolysis a yield of approximately 45% DG was obtained. 66% of total DG was 1,3-DG.

Keywords

Pseudomonas lipase;diglyceride;glycerol;triglyceride;free fatty acid

References

  1. Biochim. Biophys. Acta. v.912 Lipase-catalyzed ester synthesis in oil-continuous microemulsions Flecher,P.D.I.;R.Freedman;B.Robinson;G.Rees;R.Schomacker
  2. J. Am. Oil Chem. Soc. v.61 Continuous synthesis of glycerides by lipase in a microporous membrane reactor Hoq,M.M.;T.Yamane;s.Shimizu
  3. Kor. J. Food Sci. Technol v.30 Producion of unique microbial lipid Sin,Y.C.
  4. JAOCS v.71 High-yield diacylglycerol formation by solid-phase enzymatic glycerolysis fo hydrogenated beef tallow Yamane,T.;S.T.Kang;K.Kawahara;Y.Koizumi
  5. Bailey's Industrial Oil and Fat Products v.2 In Fat splitting, esterification, and interesterification Sonntag,N.O.N.
  6. Biochim. Biophys. Acta v.912 Lipase-catalysed ester synthesis in oil-continuous microemulsions Paul,D.I.;f.Robert;b.Freedman;H.Robinson
  7. J. Am. Chem. Soc. v.59 Glycerolysis of fats and methyl esters Sonntag,N.O.V.
  8. J. Am. Oil Chem. Soc. v.66 Enzymatic glycerolysis of a triglyceride in aqueous and nonaqueous microemulsions Holmberg,K.;b.Lasesen;M.Strak
  9. J. Am. Oil Chem. Soc. v.68 High yield enzymatic glycerolysis of fats and oils McNeill,G.P.;S.Shimazu;T.Yamane
  10. J. Jpn. Oil Chem. Soc. v.35 Continuousglycerolysis of fat by lipase in microporous hydrophobic membrane reactor Yamane,T.;M.M.Hoq;S.Ithoh;S.Shimizu
  11. Appl. Biochem. Biotechnol. v.50 Production of glycerides from glycerol and fatty acids by native lipase of Nigella sativa seed Sunna,M.L.Dandik;H.A.Aksoy
  12. J. Ferment. Bioeng v.80 Esterification of glycosides by a mono-and diacylglycerol lipase from Penicillium camembertii a comparison of the products with Candida cylindracea lopase Hirofumi,N.;S.Kitabata;S.Yuji;N.Masaki;T.Yoshio
  13. J. Lipid Res. v.2 Separation of lipid classes by chromatography on florisil Karroll,K.
  14. Development of enzymatic reactor for production of emulsifier and useful materials Korea Advanced Institute of Science & Technology
  15. Yeast v.14 Candida rugosa lipases. Molecular Biology and Versatility in Biotechnology Benjamin,S.;A.Pandey
  16. Kor. J. Food Sci. Technol v.30 Production and use of functional lipids Yun,S.H.;K.S.Kim;S.W.Zoe
  17. Kor. J. Food Sci. Technol. v.30 Development and use of carbohydrate polyesters Jung,H.Y.
  18. LIPIDS v.18 Quantitative determination of Tri-, Di-, Monooleins and Free Oleic Acid by the Thin Layer Chromatography-Flame Ionization Detector System Using Internal Standards and Boric Acid Impregnated Chromarod Tatara,T.;T.Fujii;T.Kawase;M.Minagawa
  19. Appl. Biochem. and Biotechnol v.82 Lipase-catalyzed production of short-chain acids trepenyl esters of interest to the food industry Francoise,L.;P.robert
  20. J. Am. Oil Chem. Soc. v.65 Enzymatic preparation of monoglycerides in microemulsion Holmberg,K.;e.Ostberg
  21. Biotechnol. Lett v.20 Rapid enzymatic production of acylglycerols from conjugated linoleic acid and glycerol in a solvent-free system Jose,A.;C.O.Arcos;G.C.Hill Jr.
  22. Determination of lipolytic activity of lipase for industrial use v.601 Japan Industry Standard
  23. J. Jpn. Oil. Chem. Soc. v.35 Glycerolysis of fat by lipase Yamane,T.;M.M.Hoq;S.Ithoh;S.Shimizu
  24. J. Am. Oil Chem. Soc. v.67 Solid phase enzymatic glycerolysis of beef tallow resultion in a high yield of monoglyceride McNeill,G.P.;S.Shimazu;T.Yamane
  25. J. Am. Chem. Soc. v.53 Food emulsifiers: Surface activity, edibility, manufacture, composition, and application Lauridsen,J.B.
  26. EP0377154, A3, B1, B2 Kao corp(JP) Preparation of diglycerides Kohori,J.;Y.Kawakara;Y.Hirota
  27. J. Org. Chem. v.64 A New approach to the stereospecific synthesis of phospholipid use of L-glyceric acid for the preparation of diacylglycerol Farzaneh,S.;D.W.Roodsari;S.P.Gregory;H.Joseph
  28. Wiedrmann: Nautrition and technic of edible oil Lee,Y.J.;H.Lars
  29. Kor. J. Food Sci. Technol. v.26 Effect of temperature on diacylglycerol production by enzymatic solid-phase glycerolysis of hydrogenated beef tallw Kang,S.T.;T.Yamane