Antiproliferative Effect and Apoptotic Induction of Bauhinia forficata Extract in Human Cancer Cells.

Bauhinia forficata 추출물의 인체 암세포에 대한 성장억제 및 세포사멸 유도 활성

  • 임혜영 (한양대학교 의과대학 유전학교실) ;
  • 이철훈 (한양대학교 의과대학 유전학교실)
  • Published : 2004.03.01

Abstract

Pata de Vaca (Bauhinia forficata) Is a tree which grows naturally in the rainforests and tropical parts of Peru and Brazil, as well as tropical zones of Asia, eastern Paraguay and northeastern Argentina. The active fraction (Pata-50) of the 70% ethanol extract from Pata de Vaca was sequentially fractionated by HP-20 Diaion column chromatography and C-18 column chromatography, and its characteristics were investigated. The growth of all cancer cells tested except for MCF-7 was Inhibited in a concentration-dependent manner by Pata-50. Its $IC_{50}$ values were estimated to be 40.4 $\mu\textrm{g}$/$m\ell$ on AGS, 51.3 $\mu\textrm{g}$/$m\ell$ on HT-29, 52.1$\mu\textrm{g}$/$m\ell$ on HepG2, 65.2$\mu\textrm{g}$/$m\ell$ on A549, and 77.5$\mu\textrm{g}$/$m\ell$ on HeLa cells. A flow cytometric analysis of HepG2 cells revealed induction of apoptosis, but cell cycle regulation was not affected. The HepG2 cell population of apoptosis region increased In a concentration-dependent manner by Pata-50.

Keywords

Bauhinia forficata;Pata de Vaca;Pata-50;antitumor activity

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