Fermentation Studies on Pseudomonas aeruginosa Producing Antifungal Secondary Metabolite, PAFS.

항진균물질을 생합성하는 Pseudomonas aeruginosa의 배양생리적 특성 연구

  • 송성기 (강원대학교 생명과학부) ;
  • 윤권상 (강원대학교 생명과학부) ;
  • 정용섭 (전북대학교 응용생물공학부) ;
  • 전계택 (강원대학교 생명과학부)
  • Published : 2004.03.01


When both fructose and galactose were added to a production medium as carbon sources, the productivity of PAFS (Psedomonas Antifungal Substance) biosynthesized by Pseudomonas aeruginosa was observed to be reduced significantly due to the well-known phenomenon of catabolite repression. In order to overcome this phenomenon by use of fermentation bioprocess, fed-batch cultivation method was examined. In addition, a high producer mutant strain, AP-20 obtained by a rational screening method was tested for its productivity of PAFS in both batch and fed-batch fermentation processes. Notably fed-batch operation showed approximately 4 fold higher PAFS productivity than traditional batch operation process. It was appeared that galactose was utilized principally for the cell growth of Pseudomonas aeruginosa whereas large portion of fructose was used for the biosynthesis of PAFS. Furthermore it was observed that composition and feeding rate of production media should be optimized even in the fed-batch fermentation bioprocess. As an example, very slow feeding of carbon sources gave rather negative effect on the production of PAFS due to significant limitation of carbon and energy sources available for the producer microorganism.


Psedomonas aeruginosa;PAFS(Pseudomonas antifungal substance);catabolite repression;fed-batch cultivation


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