Analysis of Enzymes of Stenotrophomonas maltophilia LK-24 Associated with Phenol Degradation

Stenotrophomonas maltophilia LK-24의 페놀분해 관련 효소

  • Kim, Jeong-Dong (Department of Life Science, Hanyang University) ;
  • Kang, Kook-Hee (Department of Food and Biotechnology, Sungkyunkwan University)
  • Published : 2004.03.01


The analysis of enzymes associated with metabolism of phenolics by Stenotrophomonas maltophilia LK-24 was conducted. To identify metabolites of phenol and phenol compound, we investigated enzymes of S. maltophilia LK-24 associated with degradation of phenolics. We found that phenol hydrolase, catechol-2.3-dioxygenase, 2-hydroxymuconic semialdehyde dehydrogenase, 2-hydroxymuconic semialdehyde hydroxylase and acetaldehyde dehydrogenase were activated. The results showed that phenolics were gone through the meta-pathway ring cleavage. The results will contribute greatly to understand metabolic pathways of phenol and it is possible to make some assessment of the feasibility of using S. maltophilia LK-24 for the treatments of phenolic-contaminated waste streams.


Stenotrophomonas maltophilia;biodegradation;phenol;meta-pathway


  1. J. Ind. Microbiol. Biotechnol. v.28 Substrate-dependent autoaggregation of Pseudomonas putida CP1 during the degradation of mono-chlorophenols and phenol Farrell,A.;B.Quilty
  2. Biodegradation v.8 Enhancement of biodegradation of phenol and a non-growth substrate 4-chlorophenol by medium augmentation with conventional carbon sources Loh,K.C.;S.J.Wang
  3. Appl. Microbiol. Biotechnol v.8 Biodegradation of Phenol by trichlorophenol-cometabolizing bacterium Park,G.T.;;J.G.Kim;S.J.Lee
  4. Appl. Environ. Microbiol. v.61 Utilization of halogenared benzenes, phenols and benzoates by Rhodococcus opacus GM-14 Zaitsev,G.M.;J.S.Uotia;I.V.Tsikol;A.G.Lobanok;M.S.Salkinoja-Salonen
  5. J. Bacteriol v.100 Phenol and benzoate metabolism by Pseudomonas putida: regulation of tangential pathway Feist,C.F.;G.D.Hegeman
  6. Biodegradation v.5 The evolution of pathway for aromatic hydrocarbon oxidation in Pseudomonas Williams,P.A.;J.R.Sayers
  7. Review Environ.Contam.Toxicol. v.146 Chlorophenols in the terrestrial environment Jenson,J.
  8. J. Bacterial. v.174 Nucleotide sequence and functional analysis of the complete phenol/3,4-dimethylphenol catabolic pathway of Pseudomonas sp. Strain CF60 Shingler,V.;J.Powlowski;U.Marklund
  9. J. Microbiol. Biotechnol v.7 Degradation of chlorophenols and phenol mixture by cooperative activities of chlorophenol-degrading strains Bae,H.S.;Y.G.Cho;S.T.Lee
  10. J. Appl. Bacteriol. v.79 Bioremediation of methyl violet and phenol from a dye-industry waste effluent using Pseudomonas spp. Sarnaik,S.;P.Kanekar
  11. Eur. J. Biochem. v.20 The meat-cleavage of catechol by Azotobacter species: 4-oxlocrotonate pathways Sala-Trepet,J.M.;W.C.Evans
  12. Anal. Biochem. v.72 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Bradford,M.M.
  13. J. Microbiol. Biotechnol v.12 Identification of Stenotrophomonas maltophilia LK-24 and its degradability of crystal violet Kim,J.D.;J.H.Yoon;Y.H.Park;F.Kawai;H.T.Kim;d.W.Lee;K.H.Kang
  14. American Society for Microbiology Metabolic and regulatory check points in phenol degradation by Pseudomonas sp. CF600. Molecular Biolgy of Pseudomonas Shinger,V.
  15. Appl. Microbiol. Biotechnol. v.46 Growth performance and pathway flux determine substrate preference of Alcaligenes eurtrophus during growth on acetate plus aromatic compound mixtrues Ampe,F.;D.Leonard;N.D.Lindley
  16. Biological degradaton and bioremediation of toxic chemicals Catabolic potenitial of Pseudomonales: a regulatory perspective Houghton,J.E.;M.S.Shanley;G.R.Chaudhry(ed.)
  17. Biophy. Res. Comm. v.60 The meta-cleavage of catechol by a thermophilic Bacillus species Buswell,J.A.
  18. J. Microbiol. Biotechnol. v.11 Identification of Yarrowia lipolytica Y103 and its degradability of phenol and 4-chlorophenol Lee,J.S.;E.J.Kang;M.O.Kim;D.H.Lee;K.S.Bae;C.H.Kim
  19. Lett. Appl. Microbiol. v.21 Aerobic degradation and dechlorination of 2-chlorophenol, 3-chlo-rophenol and 4-chlorophenol by a Pseudomonas pickettii strain Fava,F.;P.M Armenate;D.Kafkewitz
  20. Appl. Microbiol. Biotechnol v.42 Phenol and its derivatives as heterotrophic substrates for microbial growh- an energetic comparison Muller,R.H.;W.Babel
  21. Appl. Microbiol. Biotechnol v.40 Utilization of side-chain precursor for penicillin biosynthesis in a high-producing strain Penicillium chrysogenun Eriksen,S.H.;B.Jensen;I.Schneider;S.Kaasgaard;J.Olsen
  22. Appl. Biochem. Biotechno. v.17 The metabolism of aromatic compounds by thermophilic Bacilli Adams,D.;D.W.Ribbons
  23. Water Res. v.36 The enhancement of 2-chlo-rophenol degradation by mixed microbial community when augmented with Pseudomonas putida CP1 Farrell,A.;B.Quilty
  24. Archive Microiol. v.174 Transformation of ortho-substituted biphenyls by Methylosinus trichosporium OB3b: substituent effect on oxidation kinetics and product formation Angela,S.L.;A.Peter;D.S.Jeremy
  25. Appl. Microbiol. Biotechnol. v.46 Growth rate-dependent expression of phenol-assimilation pathways in Alcaligenes eutrophus LMP 134, influence of formate as an auxiliary energy source Muller,R.H.;W.Babel
  26. Appl. Microbiol. Biotechnol v.50 Simultaneous degradation of chloro- and methyl-substituted aromatioc compounds: competition between Pseudomonas strains suing the ortho - and meta-pathway or the ortho-pathway exclusively Franck Mokroa,A.C.;E.Schmidt
  27. Appl. Microbiol. Biotechnol. v.29 Kinetics of the simultaneous batch degradation of p-cresol and phenol by Pseudomonas putida Hutchinson,D.H.;C.W.Robinson
  28. Einfuhrung in die Gewassermikrobuiolgie Reichardt,W
  29. Microbiology v.146 Arrangement and regulation of the genes for meta-pathway enzymes required for degradation of phenol in Comamonas testosterroni TA441 Arai,H.;T.Ohishi;M.Y.Chang;T.Kudo
  30. Biodegradation v.7 Biodegradation of the mixtures of 4-chlorophenol and phenol by Comamonas testosteroni CPW 301 Sung,B.H.;J.M.Lee;Y.B.Kim;S.T.Lee
  31. Enzyme Microbiol. Technol. v.23 Meta-pathway degradation of phenolics by thermophilic Bacilli Saiqa,A.;F.L.Roberto;A.C.Don
  32. Microbiology v.142 Flu limitation in orthopathway of benzoate degradation of Alcaligenes eutrophus: metabolite overflow and induction of the meta-pathway at high substrate concentrations Ampe,F.;N.D.Lindley
  33. J. Bacteriol. v.124 Metabolism by phenols and cresols by Bacillus stearothermophilus Buswell,J.A.
  34. J. Bacteriol v.107 Dissimilation of aromatic compounds by Alcaligenes eutrophus Johnson,B.F.;R.Y.Stanier