Isolation and Characterization of Thermostable Xylanase-producing Paenibacillus sp. DG-22.

내열성 Xylanase를 생산하는 Paenibacillus sp. DG-22 균주의 분리 및 효소 특성

  • Published : 2004.03.01

Abstract

A new moderate thermophilic bacterial strain DG-22 which produces thermostable xylanase was isolated from a timber yard soil in Kyungju, Korea. On the basis of morphological, biochemical and phylogenetic studies the new isolate was identified as a Paenibacillus species. Production of xylanase in this strain was strongly induced by adding xylan to the growth medium and repressed by glucose or xylose. No cellulase activity was detected. The temperature and pH for optimum activity were 8$0^{\circ}C$ and 5.0-5.5, respectively. The crude xylanase was stable at $60^{\circ}C$ and retained 60% of initial activity after 2h at $70^{\circ}C$. Zymogram analysis of the culture supernatant showed two xylanase active bands with molecular masses of 22 and 30 kDa.

Keywords

Isolation;thermostable xylanase;Paenibacillus sp.

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