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돌나물 엽절편으로부텨 캘러스 형성 및 식물체 분화에 미치는 생장조절물질의 영향

Effects of Growth Regulators on Callus Induction and Plant Regeneration from Leaf Explants of Sedum sarmentosum

  • 안정호 (원광대학교 식물자원과학부 생명자원과학연구소) ;
  • 이승엽 (원광대학교 식물자원과학부 생명자원과학연구소)
  • Ahn, Jeong-Ho (Institute of Life Science and Natural Resources, Division of Plant Resources Science) ;
  • Lee, Seung-Yeob (Institute of Life Science and Natural Resources, Division of Plant Resources Science)
  • 발행 : 2004.03.01

초록

돌나물 (Sedum samentosum)의 기내 식물체 재분화 체계를 확립하기 위하여, 생장조절물질을 다르게 조합한MS배지에 잎절편을 치상한 후,캘러스 유기 및 식물체 분야에 미치는 생장조절물질의 영향을 조사하였다. 캘러스 유기는 3.0mg/L 2,4-D와 1.0mg/L BA첨가 배지에서 가장 높은 캘러스 유기율을 보였으나, NAA와 BA 혼합첨가 배지에서는 캘러스 형성율이 0∼3%이하로 극히 낮았다. 식물체 분화는 NAA 단독첨가 배지에서는 전혀 분화되지 않았으며,0.2mg/L NAA와 3.0mg/L BA첨가배지에서 가장 양호하였다. 캘러스를 식물체 분화배지에 계대배양 후 50일까지는 신초수 증가와 함께 (17.2개/캘러스),식물체의 기형이나 잎비대 현상도 적어 식물체 분화배지로 가장 적합하였다. 분화 식물체는 생장조절물질을 첨가하지 않은 MS기본배지로 계대배양하였을 때 발근 및 생육이 양호하였다.

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  1. Effect of Cytokinin and Putrescine on Plant Regeneration from Leaf Explant of Rhodiola sachalinesis A. Bor vol.32, pp.3, 2005, https://doi.org/10.5010/JPB.2005.32.3.195
  2. In vitro propagation of endangered species, Hylotelephium ussuriense (Kom.) H. Ohba vol.41, pp.1, 2014, https://doi.org/10.5010/JPB.2014.41.1.38
  3. Plant regeneration and soil acclimatization through photoautotrophic culture from leaf explant of a rare species in Sedum tosaense Makino vol.40, pp.2, 2013, https://doi.org/10.5010/JPB.2013.40.2.079
  4. Effect of plant growth regulators on plant regeneration from the Sedum rotundifolium D. Lee vol.37, pp.1, 2010, https://doi.org/10.5010/JPB.2010.37.1.084
  5. Influence of Benzyladenine and Thidiazuron on Shoot Regeneration from Leaf and Shoot tip Explants of Sedum sarmentosum Bunge vol.59, pp.0, 2016, https://doi.org/10.1590/1678-4324-2016150717