- Volume 17 Issue 7
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Full Length cDNA, Genomic Organizations and Expression Profiles of the Porcine Proteasomal ATPases PSMC5 Gene
- Wang, Y.F. (Lab of Molecular Biology and Animal Breeding, School of Animal Science and Technology, Huazhong Agricultural University) ;
- Yu, M. (Lab of Molecular Biology and Animal Breeding, School of Animal Science and Technology, Huazhong Agricultural University) ;
- Liu, B. (Lab of Molecular Biology and Animal Breeding, School of Animal Science and Technology, Huazhong Agricultural University) ;
- Fan, B. (Lab of Molecular Biology and Animal Breeding, School of Animal Science and Technology, Huazhong Agricultural University) ;
- Wang, H. (Lab of Molecular Biology and Animal Breeding, School of Animal Science and Technology, Huazhong Agricultural University) ;
- Zhu, M.J. (Lab of Molecular Biology and Animal Breeding, School of Animal Science and Technology, Huazhong Agricultural University) ;
- Li, K. (Lab of Molecular Biology and Animal Breeding, School of Animal Science and Technology, Huazhong Agricultural University)
- Received : 2003.11.05
- Accepted : 2004.03.17
- Published : 2004.07.01
PSMC5 subunit, which belongs to the 26S proteasomal subunit family, plays an important role in the antigen presentation mediated by MHC class I molecular. Full-length cDNA of porcine PSMC5 was isolated using the in silico cloning and rapid amplification of cDNA ends (RACE). Amino acid was deduced and the primary structure was analyzed. Results revealed that the porcine PSMC5 gene shares the high degree of sequence similarity with its mammalian counterparts at both the nucleotide level and the amino acid level. The RT-PCR was performed to detect the porcine PSMC5 expression pattern in seven tissues and the result showed that high express level was observed in spleen, lung, marrow and liver while the low express level was in muscle. The full-length genomic DNA sequence of porcine PSMC5 gene was amplified by PCR and the genomic structure revealed that this gene was comprised by 12 exons and 11 introns. Best alignment of the cDNA and genomic exon DNA sequence presents 4 mismatches and this information potentially bears further study in gene polymorphisms.
Sequences Analysis;Expression Profiles;Porcine;PSMC5
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