- Volume 17 Issue 10
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Establishment and Identification of a Debao Pony Ear Marginal Tissue Fibroblast Cell Line
- Zhou, X.M. (Institute of Animal Sciences, Chinese Academy of Agricultural Sciences) ;
- Ma, Y.H. (Institute of Animal Sciences, Chinese Academy of Agricultural Sciences) ;
- Guan, W.J. (Institute of Animal Sciences, Chinese Academy of Agricultural Sciences) ;
- Zhao, D.M. (College of Veterinary Medicine, China Agricultural University)
- Received : 2003.12.15
- Accepted : 2004.05.21
- Published : 2004.10.01
The Debao pony ear marginal tissue fibroblast cell line (NDPEM 2/2) was uccessfully established using either primary explant technique or collagenase technique. The characterizations of the cell line were identified as following: the cells were adherent and of density limitation; population doubling time (PDT) of cells made with the two techniques were 35.9 h and 48 h, respectively; chromosome analysis showed that the frequency of cell chromosome number to be 2n=64 was 91.3%-92.8%. Confirmed by isoenzyme analysis, this cell line had no cross- contamination. Tests for microbial contamination from bacteria, fungi, virus or mycoplasma were negative. This newly established cell line meets all the standard quality controls of ATCC. It will provide a precious genetic resource for the conservation of the Debao pony breed, as well as effective experimental material for genetic studies on Debao ponies.
Supported by : National Science Foundation of China
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