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Establishment and Identification of a Debao Pony Ear Marginal Tissue Fibroblast Cell Line

  • Zhou, X.M. (Institute of Animal Sciences, Chinese Academy of Agricultural Sciences) ;
  • Ma, Y.H. (Institute of Animal Sciences, Chinese Academy of Agricultural Sciences) ;
  • Guan, W.J. (Institute of Animal Sciences, Chinese Academy of Agricultural Sciences) ;
  • Zhao, D.M. (College of Veterinary Medicine, China Agricultural University)
  • Received : 2003.12.15
  • Accepted : 2004.05.21
  • Published : 2004.10.01

Abstract

The Debao pony ear marginal tissue fibroblast cell line (NDPEM 2/2) was uccessfully established using either primary explant technique or collagenase technique. The characterizations of the cell line were identified as following: the cells were adherent and of density limitation; population doubling time (PDT) of cells made with the two techniques were 35.9 h and 48 h, respectively; chromosome analysis showed that the frequency of cell chromosome number to be 2n=64 was 91.3%-92.8%. Confirmed by isoenzyme analysis, this cell line had no cross- contamination. Tests for microbial contamination from bacteria, fungi, virus or mycoplasma were negative. This newly established cell line meets all the standard quality controls of ATCC. It will provide a precious genetic resource for the conservation of the Debao pony breed, as well as effective experimental material for genetic studies on Debao ponies.

Keywords

Debao Pony;Ear Marginal Tissue;Fibroblast Cell Line;Primary Explant Technique;Collagenase Technique

Acknowledgement

Supported by : National Science Foundation of China

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