Portal Absorption of Feed Oligo-peptides in Chickens

  • Wang, Lijuan (College of Animal Science & Technology, China Agricultural University) ;
  • Ma, Qiugang (College of Animal Science & Technology, China Agricultural University) ;
  • Cheng, Ji (College of Animal Science & Technology, China Agricultural University) ;
  • Guo, Baohai (College of Animal Science & Technology, China Agricultural University) ;
  • Yue, Hongyuan (College of Animal Science & Technology, China Agricultural University)
  • Received : 2003.12.04
  • Accepted : 2004.05.17
  • Published : 2004.09.01


The effect of duodenal infusion with feed oligo-peptide solution on portal absorption of amino acids was investigated in poultry under unanaesthetized conditions. Four peptide solutions were used in the experiment: enzymatic hydrolysates from fish meal, soybean meal, cottonseed meal and rapeseed meal proteins with average molecular weights less than 3,000 Da and 1,000 Da, respectively. Intestinal absorptions of these oligo-peptide solutions were compared by determining the concentration of free amino acid (FAA) in portal blood after the duodenal administrations of oligo-peptide solutions. Absorptive intensity and balance were used to estimate the intestinal absorption rate of amino acids. The absorptive intensities of amino acids were highest for the fish and soybean meal oligo-peptides. The ratios of amino acids absorbed in the portal blood from fish and soybean meal oligo-peptides were more similar to the composition of the infused amino acids than that observed from the cottonseed and rapeseed meal oligo-peptides. A positive correlation was found between absorption rate and proportion of PAA in the oligo-peptides. The higher absorption rate could be contributed to the higher proportion of peptide bound amino acids (PAA). The results suggest that fish and soybean meal protein are significantly more easily hydrolyzed into oligo-peptides (p<0.05) in the gastrointestinal tracts of poultry and as such can be utilized more effectively by body tissues.


Chickens;Oligo-peptide;Intestinal Absorption;Duodenal Infusion;Portal Amino Acid


  1. Burston, D. J., M. Addison and D. M. Matthews. 1972. Uptake of depeptide containing basic and acidic amino acids by rat small intestine in vitro. Clin. Sci. 43:823.
  2. Daniel, H., M. Boll and U. Wenzel. 1994. Physiological importance and characteristics of peptide transport in intestinal epithelial cells. In: (Ed. W. B. Souffrant and H. Hagemeister). VIth international Symposium on Digestive Physiology in Pigs.Vol. 1:1-7. Dummerstorf Pub.
  3. Rerat, A., C. S. Nunes, F. Mendy and L. Roger. 1988. Amino acid absorption and production of pancreatic hormones in anaesthetized pigs after duodenal infusions of a milk enzymatic hydrolysate or of free amino acids. Br. J. Nutr. 60:121-136.
  4. Savoie, L., R. Charbonneau and C. Parent. 1989. In vitro amino acid digestibility of food proteins as measured by the digestion cell technique. Plant-foods for human nutr. 39:93-107.
  5. Feng, X.Y., C. Ji and H. Jiang. 2002. Relationship between amino acid availability and hydrolyzed peptide quantity in poultry feed. Journal of China Agricultural University 7(1):107-113.
  6. Webb. K. E. 1990. Intestinal absorption of protein hydrolysis products: A review. J. Anim. Sci. 68:3011-3022.
  7. Matthews, D. M. 1975. Intestinal absorption of peptides. Physiol. Rev. 55:527.
  8. Hara, H., R. Funabili, M. Iwata and K. I. Yamazaki. 1984. Portal absorption of small peptides in rats under unrestrained conditions. J. Nutr. 114:1122.
  9. Silk, D. B. A., G. K. Grimble and R. G. Rees. 1985. Protein digestion and amino acid and peptide absorption. Proc. Nutr. Sci. 44:63.
  10. Webb, K. E., Jr. J. C. Matthews and D. B. DiRienzo. 1992. Peptide absorption -a review of current concepts and future perspectives. J. Anim. Sci. 70:3248.
  11. Duncan, D. B. 1955. Multiple range and multiple F tests. Biometrics 11:1-42.
  12. Matthews, D. M. Rate of peptide uptake by small intestine. 1972. In: (Ed. K. Ellion and M. O'Connor) CIBA Symposium: peptide transport in bacteria and mammalian gut. pp. 71-88. Associated scientific publishers, Amsterdam.
  13. Le, G. W., Y. H. Shi, J. Xie, Z. H. Wang and F. Yang. 1997. The effect of duodenal infusion of casein hydrolysate- COP or equivalent AA mixture on the intestinal amino acids absorption and portal plasma peptides of chicks. Acta Zoonutrimenta Sinica. 9(4):14-23.
  14. Kim, Y. S., W. Birtwhistle and Y. W. Kim. 1972. Peptide hydrolases in the brush border and soluble fractions of small intestinal mucosa of rat and man. J. Clin. Invest. 51:1419.

Cited by

  1. Preventive Effects of Collagen Peptide from Deer Sinew on Bone Loss in Ovariectomized Rats vol.2014, pp.1741-4288, 2014,
  2. Creation of thermostable polypeptide cassettes for amino acid balancing in farm animal rations vol.53, pp.6, 2017,