DOI QR코드

DOI QR Code

Antioxidant Enzymes in Postpartum Anoestrus Buffaloes Supplemented with Vitamin E and Selenium

  • Anita, Anita ;
  • Singha, S.P.S. ;
  • Dhillon, K.S. ;
  • Nayyar, Shashi
  • Received : 2002.09.17
  • Accepted : 2003.04.04
  • Published : 2004.05.01

Abstract

The 15 buffaloes were divided into three groups, viz. group 1: normal cyclic buffaloes; group 2: postpartum anoestrus buffaloes and group 3: post partum anoestrus buffaloes supplemented with intramuscular injections of Vit. E.-care Se containing 500 mg $\alpha$-tocopheryl acetate and 15 mg selenium at weekly intervals for two months. The postpartum anoestrus buffaloes had significantly higher levels of erythrocytic lipid peroxidation, superoxide dismutase and glucose-6 phosphate dehydrogenase activities but lower glutathione peroxidase activity as compared to normal cyclic buffaloes. The supplementation of vitamin E and selenium lowered the level of erythrocytic lipid peroxidation, superoxide dismutase and glucose-6 phosphate dehydrogenase activities but it had no effect on whole blood selenium and erythrocytic gluathione peroxidase activity. All the animals in group 3 became cyclic and showed 60% conception rate.

Keywords

Vitamin E;Selenium;Anoestrus;Antioxidant Enzymes

References

  1. Brezezinska, S. E., J. Miller, I. D. III Quigley, J. R. Moore and F. C. Madsen. 1994. Antioxidant status of dairy cows supplemented prepartum with vitamin E and selenium. J. Dairy Science 77:3087-3095.
  2. Nishikimi, M., N. A. Rao. and K. Yasgi. 1972. The occurrence of the superoxide anion in the reaction of reduced phenazine methsulfate and molecular oxygen. Biochem. Biophys. Res. Commun. 46:849-854.
  3. Koh, T. S. and T. H. Benson. 1983. Critical reappraisal of fluorometric method for determination of selenium in biological materials. Assoc. Off. Anal. Chem. J. 66:918-925.
  4. Deutsch, J. 1978. Maleimide as an inhibitor in measurement of erythrocyte glucose-6-phosphae dehydrogenase activity. Clin. Chem. 24:885-889.
  5. Placer, Z. A., L. L. Cushman and B. C. Johnson. 1966. Estimation of product of lipid peroxidation (Malonyl dialdehyde) in biochemical systems. Anal. Biochem. 16:359-364.
  6. Hafeman, D. G., R. A. Sunde and W. G. Hoekstra. 1974. Effect of dietary selenium on erythrocyte and liver glutathione peroxidase in the rat. J. Nutr. 104:580-587.
  7. Walsh, D. M., S. Kennedy, W. J. Blanchflower, E. A. Goodall and D. G. Kennedy. 1993a. Vitamin E and selenium deficiencies increase indices of lipid peroxidation in rumiant calves. J. vit. Nutr.Res. 63:188-194.
  8. Walsh, D. M., D. G. Kennedy, E. A. Goodall and S. Kennedy. 1993b. Antioxidant enzyme activity in the muscle of calves depleted of vitamin E or selenium or both. Br. J. Nutr. 70:621-630.
  9. Bieleski, H. K., P. Weber and H. E. Weiss. 1997. Antioxidative vitamins in prevention. Vitamin: Physiologie Patho Physiology Theraphie: 206-228.
  10. Singh, S., T. P. Singh, M. L. Bansal and R. Kumar. 1991. Statistical Methods for Research Workers. pp.310-312. Kalyani Publisher, New Delhi.
  11. Kim, H. S., J. M. Lee, S. B. Park, S. G. Jeag, J. K. Jung and K. S. Im. 1997. Effect of vitamin E and selenium administration on the reproductive performance in the dairy cows. Asian-Aust. J. Anim.Sci. 10:308-312.
  12. Pironi, L., E. Ruggeri, C. Zolezzi and L. Sarvarino.1998. Lipid peroxidation and antioxidant status in adult receiving lipid based home parental nutrition. Am. J. Clin. Nutr. 68:888-893.

Cited by

  1. Characterization and adsorption of arsenate and selenite onto Kemiron vol.43, pp.10, 2008, https://doi.org/10.1080/10934520802171741