- Volume 17 Issue 12
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Multiple Ovulations and In vitro Fertilization in the Domestic Fowl (Gallus domesticus)
- Han, Haitang (Department of Biochemistry and Molecular Biology, China Agricultural University) ;
- Zhao, Chen (Department of Biochemistry and Molecular Biology, China Agricultural University) ;
- Li, Zandong (Department of Biochemistry and Molecular Biology, China Agricultural University)
- Received : 2003.10.23
- Accepted : 2004.05.25
- Published : 2004.12.01
The aim of this study was to obtain mature ova or embryos at a single cell stage, which can be used in avian transgenesis and nuclear transfer through multiple ovulations, in vitro fertilization and culture. Chicken anterior pituitary extract (CAPE) or acetone-dried chicken anterior pituitary extract (ACAPE) was used to induce multiple ovulations in hens pretreated with pregnant mare' serum gonadotrophin (PMSG). In vitro fertilization of the multiple ovulated ova was performed by inseminating sperm onto the germinal disks in m-Ringer' solution and incubating the ova at 41
Supported by : National Natural Science Foundation of China
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