Effect of α-Tocopherol Supplementation on Plasma Levels of Antioxidant Vitamins in Anestrus Buffalo Heifers (Bubalus bubalis)

  • Kahlon, R.S. (Department of Veterinary Physiology, College of Veterinary Science, Punjab Agricultural University) ;
  • Singh, Rajvir (Department of Veterinary Physiology, College of Veterinary Science, Punjab Agricultural University)
  • Received : 2003.10.11
  • Accepted : 2004.04.23
  • Published : 2004.08.01


The present investigation was undertaken to study the status of plasma antioxidant vitamins in normal cycling and $\alpha$-tocopherol supplemented anestrus buffalo heifers. The pre-supplementation plasma levels ($\mu$mol/L) of vitamin E and $\beta$-carotene were significantly (p<0.05) lower and of vitamin C was significantly (p<0.05) higher in anestrus heifers (4.06$\pm$0.07; 4.56$\pm$0.17; 21.04$\pm$0.21) when compared to normal cycling ones (4.92$\pm$0.05; 6.76$\pm$0.12; 14.24$\pm$0.16). The oral supplementation of$\alpha$-tocopherol at 3,000 mg per week per animal in anestrus heifers resulted in a significant (p<0.01) increase in vitamin E and $\beta$-carotene levels and a significant (p<0.01) decrease in vitamin C concentration. Results indicated that supplementation of $\alpha$-tocopherol to anestrus buffalo heifers improved the antioxidant status by mitigating the harmful effects of free radical induced oxidative stress.


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