DOI QR코드

DOI QR Code

Effect of α-Tocopherol Supplementation on Plasma Levels of Antioxidant Vitamins in Anestrus Buffalo Heifers (Bubalus bubalis)

  • Kahlon, R.S. (Department of Veterinary Physiology, College of Veterinary Science, Punjab Agricultural University) ;
  • Singh, Rajvir (Department of Veterinary Physiology, College of Veterinary Science, Punjab Agricultural University)
  • Received : 2003.10.11
  • Accepted : 2004.04.23
  • Published : 2004.08.01

Abstract

The present investigation was undertaken to study the status of plasma antioxidant vitamins in normal cycling and $\alpha$-tocopherol supplemented anestrus buffalo heifers. The pre-supplementation plasma levels ($\mu$mol/L) of vitamin E and $\beta$-carotene were significantly (p<0.05) lower and of vitamin C was significantly (p<0.05) higher in anestrus heifers (4.06$\pm$0.07; 4.56$\pm$0.17; 21.04$\pm$0.21) when compared to normal cycling ones (4.92$\pm$0.05; 6.76$\pm$0.12; 14.24$\pm$0.16). The oral supplementation of$\alpha$-tocopherol at 3,000 mg per week per animal in anestrus heifers resulted in a significant (p<0.01) increase in vitamin E and $\beta$-carotene levels and a significant (p<0.01) decrease in vitamin C concentration. Results indicated that supplementation of $\alpha$-tocopherol to anestrus buffalo heifers improved the antioxidant status by mitigating the harmful effects of free radical induced oxidative stress.

Keywords

Antioxidant Vitamins;$\alpha$-Tocopherol;Anestrus;Buffalo Heifers

References

  1. Burton, G. W. and K. U. Ingold. 1984. $\beta$-Carotene: an unusual type antioxidant. Science 224:569-573.
  2. Goto, K., O. Kajisa, K. Ezoe, Y. Nakanishi, A. K. Ogaw, M. Tasaki, H. Ohta, S. Inohae, S. Tateyama and T. Kawabata. 1989. Relationship between plasma $\beta$-carotene concentration and embryo quality in superovulated Japanese Black cattle. Memoirs Fac. Agril Kagoshima Univ.25:113-117.
  3. Grandhi, R. P., M. W. Smith, M. Frigg and P. A. Thacker. 1993. Effect of supplemental vitamin E during prepubertal development and early gestation on reproductive performance and nutrient metabolism in gilts. Can. J. Anim. Sci. 73:593-603.
  4. Jukola, E., J. Hakkarainen, H. Saloniemi and S. Sankari. 1996. Blood selenium, vitamin E, Vitamin A and $\beta$-carotene concentrations and udder development, fertility treatments and fertility. J. Dairy Sci. 79:838-845.
  5. Liebler, D. C. and J. A. Burr. 1995. Antioxidant stoichiometry and the oxidative fate of vitamin E in peroxy radical scavenging reactions. Lipids 30:789-793.
  6. Wang, J. Y., C. B. Hafi and L. L. Larson. 1988. Effect of supplemental $\beta$-carotene on luteinizing hormone released in response to gonadotropin-releasing hormone challenge in ovariectomized Holstein cows. J. Dairy Sci. 71:498-504.
  7. Machlin, L. J. and A. Bendich. 1987. Free radical tissue damage: protective role of antioxidant nutrients. Fed. Am. Soc. Exp. Biol. J. 1: 441-445.
  8. Chan, A. C. 1993. Partners in defense, Vitamin E and Vitamin C. J. Physiol. Pharmacol. 71:725-731. https://doi.org/10.1139/y93-109
  9. Machlin, L. J. 1991. Vitamin E. In: (Ed. L. J. Machlin) Handbook of Vitamins. 2nd edn, pp. 99-144. Marcel Dekker, New York.
  10. Krinsky, N. I. 1993. Actions of carotenoids in biological systems. Ann. Rev. Nutr. 13:561-587. https://doi.org/10.1146/annurev.nu.13.070193.003021
  11. Kahlon,R. S., S. P. S. Sodhi, Singh Rajvir and Singh Narinder 2002. Osmotic fragility of erythrocytes in normal cycling and $\alpha$-tocopherol supplemented anestrus buffalo heifers. SARAS J. Livestock & Poultry Production 18 (3-4):30-35.
  12. Weiss, W. P., J. S. Hogan, K. L. Smith and S. N. Williams. 1994. Effect of dietary fat and vitamin E on $\alpha$-tocopherol and $\beta$-carotene in blood of peripartum cows. J. Dairy Sci. 77:1422-1429.
  13. Stahl, W. and H. Sies. 1993. Physical quenching of singlet oxygen and cis – trans isomerization of carotenoids. Ann. New York Acad. Sci. 691: 10-19. https://doi.org/10.1111/j.1749-6632.1993.tb26153.x
  14. Mert, N., R. Vural, U. Gunsen and M. Akandir. 1992. Relationship of anoestrus with plasma $\beta$-carotene, vitamin A and vitamin E levels in Thoroughbred crosses. Veteriner Fakultesi Dergisi Uludag Universitesi 11:19-23.
  15. Larson, R. A. 1997. Carotenoids and related polyenes. In: (Ed. R. A. Larson) Naturally Occurring Antioxidants. pp. 179-189. Lewis Publishers, New York.
  16. Papas, A. M. 1996. Determinants of antioxidant status in humans. Lipids 31:S77-S82.
  17. Garnsworthy, P. C. 1988. Nutrition and Lactation in the Dairy cow. pp. 140-145. Butterworths, London.
  18. Kayden, H. J., C. K. Chow and L. K. Bjornson. 1973. Spectrophotometric method for determination of tocopherol in red blood cells. J. Lipid Res. 14:533-540.
  19. Draper, H. H. 1990. Nutritional modulation of oxygen radical pathology. Adv. Nutr. Res. 8:119-145.
  20. Kahlon, R. S. 1998. Studies on antioxidant status of normal cycling, delayed pubertal and anoestrus buffaloes (Bubalus bubalis). Ph. D. dissertation, Punjab Agricultural University, Ludhiana, India.
  21. Lotthammer, K. H. 1975. Eierstocks-und Gebarmuttererkrankungen bei subklinischen stoffwechselstorungen der Milchkuhe. Praktische Tierarzt Sonderheft collegium Veternarium 56:24-29.
  22. Lotthammer, K. H., B. C. Cook and H. Friesecke. 1978. Importance of $\beta$-carotene for bovine fertility. In: Roche Symposium. London, Roche, Switzerland.
  23. Baker, H. and O. Frank. 1968. Clinical Vitaminology: Methods and Interpretation. pp. 153-168. Interscience Publishers, London.
  24. Barnes, M. M. C. and A. J. Smith. 1975. The effect of vitamin E deficiency on some enzymes of steroid hormone biosynthesis. Int. J. Vit. Nutr. Res. 45:396-402.
  25. Ezzo, O. H. 1995. The effect of vitamins and selenium supplementation on serum vitamin levels and some reproductive patterns in Egyptian buffaloes during pre and postpartum periods. Buffalo J. 11:103-107.
  26. Rock, C. L., R. A. Jacob and P. E. Bowen.. 1996. Update on the biological characteristics of the antioxidant micronutrients: vitamin C, vitamin E and the carotenoids. J. Am. Diet Assoc. 96:693-702.
  27. Kahlon, R. S. and R. Singh. 2003. Status of antioxidant enzymes in normal cycling and $\alpha$-tocopherol supplemented anestrus buffalo heifers (Bubalus bubalis). Asian-Aust. J. Anim. Sci. 16(2):217-221.