Effect of Recombinant Bovine Somatotropin (Boostin-250) on Blood Metabolites and Milk Yield of Lactating Buffaloes

  • Mishra, A. (Centre of Advanced Studies, Division of Physiology, Indian Veterinary Research Institute) ;
  • Shukla, D.C. (Centre of Advanced Studies, Division of Physiology, Indian Veterinary Research Institute)
  • Received : 2003.10.15
  • Accepted : 2004.05.29
  • Published : 2004.09.01


In order to investigate the effect of recombinant bovine somatotropin (rbST) on blood metabolites and milk yield of lactating buffaloes, 30 lactating buffaloes after acclimatization for 30 days were divided into 2 groups as control (n=10) and experimental (n=20). Animals were injected 250mg of rbST (Boostin-250) on 0, $14^{th}$ and $28^{th}$ day subcutaneously at ischiorectal fosse, where as control animals were given placebo of 2 ml normal saline. Fortnightly blood samples were collected from 15 days prior to 60 days post injection to estimate different blood metabolites. Daily milk yield was recorded and weekly average yield of each animal was calculated. From this study, it was found that blood metabolites such as glucose, triglycerides, total proteins, albumin, globulin and electrolytes like sodium and potassium were not altered by rbST injection. However, there was a significant (p<0.05) decrease in blood urea nitrogen (BUN) concentration in experimental group as compared to that of control group. The weekly average milk yield was significantly (p<0.001) higher (25%) in rbST injected group over the control group.


rbST;Blood Metabolites;Milk Yield;Buffaloes


  1. Doumas, B. T., W. A. Watson and A. G. Biggs. 1971. Clinical chemistry Acta, 31:87.
  2. Fullerton, F. M. 1989. Cardiovascular responses and mammary substrate uptake in Jersey cows treated with pituitary derived growth hormone during lactation. J. Dairy Resea. 56(1):27-35.
  3. Lough, D. S., L. D. Muller, R. S. Kensinger, T. F. Sweeney and L. C(Jr). Griel. 1988. Effct of added dietary fat and bovine somatotropin on the performance and metabolism of lactating dairy cows. J. Dairy Sci. 71:1161-1169.
  4. Rodkey, F. L. 1965. Direct spectrophotometric determination of albumin in human serum, clinical chemistry, 11:478-487.
  5. Abas, I. and H. Ozpinar. 2001. Studies on the effect of bovine somatotropin hormone on milk yield, milk composition and health of dairy cows. Turk Veterinerlik-ve-Hayvancilik-Deraisi, 25(1):65-73.
  6. Tanwattna, P., S. Chanpongsang and N. Chaiyabuter. 2003. Effect of exogenous bovine somatotropin on mammary function of late lactating crossbred Holstein cows. Asian-Aust. J. Anim. Sci. 16(1):88-95.
  7. Bauman, D. E., M. J. De Geeter, C. J. Peel, G. M. Lanza and R. C. Gorewit. 1982. Effect of recombinantly derived bovine growth hormone (bGH) on lactational performance of high yielding dairy cows. J. Dairy Sci. 65(Suppl. 1):121(Abstr.).
  8. Kweon, U. G., K. T. Nam, H. S. Kim, S. K. Yun, J. S. Kim and B. J. Hang. 2000. Effect of rbST (recombinat bovine somatotropin) injection time on haemotological values and blood metabolic substrates in holstein steers. Kor. J. Anim. Sci. 42(3):269-278.
  9. Vicini, J. L., D. S. Hudson, W. J. Cole, M. A. Miller, P. J. Eppard, T. C. White and R. J. Collier. 1990. Effect of acute challenge with an extreme dose of somatotropin in prolonged release formulation on milk production and health of diary cattle. J. Dairy Sci. 73:2093-2102.
  10. Lean, I. J., R. L. Baldwin, H. F. Troutt, M. L. Bruss, J. C. Galland, T. B. Farver, J. Rostami, L. D. Weaver and C. A. Holmberg. 1992. Impact of bovine somatotropin administration beginning at day 70 of lactation serum metabolites, milk contituents and production in cows previously exposed to exogenous somatotropin. Am. J. Vet. Res. 53(5):731-741.
  11. Bucolo, G. and M. David. 1973. Quantitative determination of serum triglycerides by the use of enzyme. Clinical Chem. 19:476-482.
  12. Bauman, D. E., P. J. Eppard, M. J. De Geeter and G. M. Lanza. 1985. Response of high producing dairy cows to long term treatment with pituitary somatotropin and recombinant somatotropin. J. Dairy Sci. 68:1352-1362.
  13. Boyd, R. D., D. E. Bauman, D. G. Fox and C. Scanes. 1991. Impact of metabolism modifiers on protein accretion and protein and energy requirements of livestock. J. Anim. Sci. 69 (Suppl.2):56-75.
  14. FAO. 2002. Food and Agriculture Organisation Trade year book. Vol. 56.
  15. Henry, R. J. 1968. Clinical chemistry, principles and technics, Harper and Row, New York, p. 268.
  16. Snedecor, G. W. and W. G. Cochran. 1989. Statistical methods. $9^{th}$ edn. The Iowa state uni.Press,Ames,Iowa.
  17. Bauman, D. E. and R. G. Vernon. 1993. Effects of Exogenous Bovine Somatotropin on lactation. Ann. Rev. Nutr. 13:437-461.
  18. Henry, R. J., D. C. Cannon and I. W. Winkelman. 1974. Clinical Chemistry, Principles and Techniques. $2^{nd}$ edition. Harper and Row Publisher, Inc. NY, p. 1288.
  19. Ludri, R. S., R. C. Upadhyay, M. Singh, J. R. M. Guneratna and R. P. Basson. 1989. Milk production in lactating buffalo receiving recombinantly produced bovine somatotropin. J. Dairy Sci. 72:2283-2287.
  20. Burton, J. L., B. W. McBride, B. W. Kennedy, J. H. Burton, T. H. Elsasser and B. Woodward. 1991. Influence of exogenous bovine somatotropin on the responsiveness of peripheral blood lymphocytes to mitogen. J. Dairy Sci. 74(3):916-928.
  21. Skarda, J., E. Markalous, J. Slaba, P. Krejei, O. Skardova and J. Zednik. 1992. Effect of methionyl bovine somatotropin in a prolonged release vehicle on milk porduction, hormone profiles and health in dairy cows. J. Dairy Res. 59(4):499-506.

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