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Cloning and Characterization of Bovine Titin-cap (TCAP) Gene

  • Yu, S.L. (Division of Animal Science & Resources, College of Agriculture and Life Sciences Chungnam National University) ;
  • Chung, H.J. (Division of Animal Science & Resources, College of Agriculture and Life Sciences Chungnam National University) ;
  • Jung, K.C. (Division of Animal Science & Resources, College of Agriculture and Life Sciences Chungnam National University) ;
  • Sang, B.C. (Division of Animal Science & Resources, College of Agriculture and Life Sciences Chungnam National University) ;
  • Yoon, D.H. (Division of Animal Genomics & Bioinformatics, National Livestock Research Institute) ;
  • Lee, S.H. (Division of Animal Genomics & Bioinformatics, National Livestock Research Institute) ;
  • Kata, S.R. (Department of Veterinary Pathology, Texas A&M University, College Station) ;
  • Womack, J.E. (Department of Veterinary Pathology, Texas A&M University, College Station) ;
  • Lee, J.H. (Division of Animal Science & Resources, College of Agriculture and Life Sciences Chungnam National University)
  • Received : 2004.02.11
  • Accepted : 2004.05.27
  • Published : 2004.10.01

Abstract

Titin-cap (TCAP), one of the abundant transcripts in skeletal muscles, was nvestigated in this study in cattle because of its role in regulating the proliferation and differentiation of myoblasts by interacting with the myostatin gene. From the 5, and 3, RACE experiments, full-length TCAP coding sequence was identified, comprising 166 amino acids. The amino acid comparison showed high sequence similarities with previously identified human (95.8%) and mouse (95.2%) TCAP genes. The TCAP expression, addressed by northern blot, is limited in muscle tissues as indicated by Valle et al. (1997). The radiation hybrid analysis localized the gene on BTA19, where the comparative human and porcine counterparts are on HSA17 and SSC12. A few muscle-related genetic disorders were mapped on HSA17 and some growth-related QTLs were identified on SSC12. The bovine TCAP gene found in this study opens up new possibilities for the investigation of muscle-related genetic diseases as well as meat yield traits in cattle.

Keywords

RACE;Northern Analysis;Radiation Hybrid Mapping;Cattle;TCAP

Acknowledgement

Supported by : Rural Development Administration

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