- Volume 17 Issue 8
DOI QR Code
Effect of Conjugated Linoleic Acid on Intestinal and Hepatic Antioxidant Enzyme Activity and Lipid Peroxidation in Broiler Chickens
- Ko, Y.H. (Department of Animal Science & Biotechnology, Regional Animal Industry Research Center (RAIRC) Jinju National University) ;
- Yang, H.Y. (Department of Animal Science & Biotechnology, Regional Animal Industry Research Center (RAIRC) Jinju National University) ;
- Jang, I.S. (Department of Animal Science & Biotechnology, Regional Animal Industry Research Center (RAIRC) Jinju National University)
- Received : 2003.11.27
- Accepted : 2004.04.29
- Published : 2004.08.01
The present study was designed to define whether dietary conjugated linoleic acid (CLA) could affect antioxidant enzymes including superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT), and glutathione S transferase (GST), and the level of malondialdehyde (MDA), a marker of lipid peroxidation, in the small intestine and liver from broiler chickens. A total of twenty-four 3 wk-old male broiler chickens were assigned to three dietary treatments (1.5% corn oil, 0.75% corn oil plus 0.75% CLA, and 1.5% CLA, isocalorically), and fed a grower-finisher diet from 22 to 35 days. In the small intestinal mucosae, the specific activities of SOD, GSH-Px, CAT, and GST, and the level of MDA were not substantially influenced by dietary CLA. In the liver, the specific activities of SOD, GSH-Px, and GST, and the level of MDA were also unaffected by dietary CLA at the level of either 0.75% or 1.5% compared with corn oil at the level of 1.5%. However, the broiler chickens fed the diet containing 1.5% CLA resulted in a significant increase in peroxisomal CAT activity and a marked decrease in total lipid and non-esterified fatty acids (NEFA) from liver tissues compared with those fed the diet containing 1.5% corn oil. In conclusion, ability of CLA to increase hepatic CAT activity suggest that dietary CLA may affect, at least in part, antioxidant defense system as well as lipid metabolism in the liver of broiler chickens.
Supported by : Korea Science and Engineering Foundation (KOSEF)
- Aebi, H. 1974. Catalase. In: Methods in Enzymatic Analysis (Ed. H. U. Bergmeyer). Academic Press. New York. pp. 673-677.
- Evans, M. E., L. M. Brown and M. K. McIntosh. 2002. Isomerspecific effects of conjugated linoleic acid on adiposity and lipid metabolism. J. Nutr. Biochem. 13:508-516.
- Jang, I., K. Chae and J. Cho. 2001. Effects of age and strain on small intestinal and hepatic antioxidant defense enzymes in Wistar and Fisher 344 rats. Mech. Ageing Dev. 122:561-570.
- O'Shea, M., R. Devery, F. Lawless, J. Murphy and C. Stanton. 2000. Milk fat conjugated linoleic acid inhibits growth of human mammary MCF-7 cancer cells. Anticancer Res. 320:3591-3601.
Rahman, S. M., Y. Wang, H. Yotsumoto, J. Cha, S. Han, S. Inoue and T. Yanagita. 2001. Effects of conjugated linoleic acid on serum leptin concentration, body-fat accumulation, and
$\beta$-oxidation of fatty acid in OLETF rats. Nutrition 17:385-390.
- Yu, L., A. Adams and M. Gabel. 2002. Conjugated linoleic acid isomers differ in their free radical scavenging properties. J. Agric. Food Chem. 50:4135-4140.
- Kramer, J. K., N. Sehat, M. E. Dugan, M. M. Mossoba, M. P. Yurawecz, J. A. Roach, K. Eulitz, J. L. Aalhus, A. L. Schaefer and Y. Ku. 1998. Distributions of conjugated linoleic acid (CLA) isomers in tissue lipid classes of pigs fed a commercial CLA mixture determined by gas chromatography and silver ion-high-performance liquid chromatography. Lipids 33:549- 558.
- Whigham, L. D., M. E. Cook and R. L. Atkinson. 2000. Conjugated linoleic acid: Implications for human health. Pharmacol. Res. 42:503-510.
- Youdim, K. A. and S. G. Deans. 1999. Dietary supplementation of thyme essential oil during the lifetime of the rat: its effect on the antioxidant status in liver, kidney and tissues. Mech. Ageing Dev. 109:163-175.
- Badinga, L., K. T. Selberg, A. C. Dinges, C. W. Corner and R. D. Miles. 2003. Dietary conjugated linoleic acid alters hepatic lipid content and fatty acid composition in broiler chickens. Poult. Sci. 82:111-116.
- Josephy, P. D., B. Mannervik and P. O. Montellano. 1997. Glutathione and Detoxification. In: Molecular Toxicology. Oxford University Press. New York, pp. 152-181.
- Stangl, G. 2000. High dietary levels of a conjugated linoleic acid mixture alter hepatic glycerophospholipid-carrying serum lipoproteins of rats. J. Nutr. Biochem. 11:184-191.
- Leung, Y. H. and R. H. Liu. 2000. Trans-10, cis-12-conjugated linoleic acid isomer exhibits stronger oxyradical scavenging capacity than cis-9,trans11-conjugated linoleic acid isomer. J. Agric. Food Chem. 48:5469-5475.
- SAS/STAT User’s Guide, Version 6, 4th Edition. Vol 2. 1989. SAS Ins., Cary, NC.
- Basu, S., A. Smedman and B. Vessby. 2000. Conjugated linoleic acid induces lipid peroxidation in humans. FEBS Letters 468:33-36.
- Ha, Y. L., J. Storkson and M. W. Pariza. 1990. Inhibition of benzo(a)pyrene-induced mouse forestomach neoplasia by conjugated dienoic derivatives of linoleic acid. Cancer Res. 50:1097-101.
- Habig, W. H., M. J. Phobst and W. B. Jakoby. 1974. Glutathione - S- transferase: The first enzymatic steps in mercapturic acid formation. J. Biol. Chem. 249:7130.
- McCord, J. M. and I. Fridovich. 1969. Superoxide dismutase. An enzymic function for erythrocuprein (hemocuprein). J. Biol. Chem. 244:6049-6055.
- Kilian, M., I. Mautsch, J. I. Gregor, P. Stahlknecht, C. A. Jacobi, I. Schimke, H. Guski and F. A. Wenger. 2002. Influence of conjugated linoleic acid vs. conventional linoleic acid on liver metastasis and hepatic lipid peroxidation in BOP-induced pancreatic cancer in Syrian hamster. Prosta. Leuko. Essential Fatty Acids 67:223-228.
- Bidlack, W. R. and A. L. Tappel. 1973. Damage to microsomal membrane by lipid peroxidation. Lipids 8:177-182.
- Dunshea, F. R., E. Ostrowska, B. Luxford, R. J. Smits, R. G. Campbell, D. N. D'Souza and B. P. Mullan. 2002. Dietary conjugated linoleic can decrease backfat in pigs housed under commercial conditions. Asian-Aust. J. Anim. Sci. 15(7):1011-1017.
- Bee, G. 2000. Dietary conjugated linoleic acids alter adipose tissue and milk lipids of pregnant and lactating sows. J. Nutr. 130:2292-2298.
- Joo, S. T., J. I. Lee, Y. L. Ha and G. B. Park. 2002. Effects of dietary conjugated linoleic acid on fatty acid composition, lipid oxidation, color, and water-holding capacity of pork loin. J. Anim. Sci. 80:108-112.
- Tappel, A. L. 1978. Glutathione peroxidase and hydroperoxides. In: Methods in Enzymology (Ed. S. Fleischer and L. Packer). Academic Press. New York. pp. 506-513.
- Chin, S. F., W. Lin, J. M. Storkson, Y. L. Ha and M. W. Pariza. 1992. Dietary sources of conjugated dienoic isomers of linoleic acid, a newly recognized class of anticarcinogenens. J. Food Comp. Anal. 5:185-197.
- Folch, J., M. Sloane and G. H. Stanley. 1957. A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem. 226:497-509.
- Wang, J. H., M. K. Song, Y. S. Son and M. B. Chang. 2002. Effect of concentrate level on the fermentation of conjugated linoleic acid and trans-octadecenoic acid by ruminal bacteria when incubated with oilseeds in vitro. Asian-Aust. J. Anim. Sci. 15(5):687-694.
- Du, M., D. U. Ahn, K. C. Nam and J. L. Sell. 2001. Volatile profiles and lipid oxidation of irradiated cooked chicken meat from laying hens fed diets containing conjugated linoleic acid. Poult. Sci. 80:235-241.