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Effect of Conjugated Linoleic Acid on Intestinal and Hepatic Antioxidant Enzyme Activity and Lipid Peroxidation in Broiler Chickens

  • Ko, Y.H. ;
  • Yang, H.Y. ;
  • Jang, I.S.
  • Received : 2003.11.27
  • Accepted : 2004.04.29
  • Published : 2004.08.01

Abstract

The present study was designed to define whether dietary conjugated linoleic acid (CLA) could affect antioxidant enzymes including superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT), and glutathione S transferase (GST), and the level of malondialdehyde (MDA), a marker of lipid peroxidation, in the small intestine and liver from broiler chickens. A total of twenty-four 3 wk-old male broiler chickens were assigned to three dietary treatments (1.5% corn oil, 0.75% corn oil plus 0.75% CLA, and 1.5% CLA, isocalorically), and fed a grower-finisher diet from 22 to 35 days. In the small intestinal mucosae, the specific activities of SOD, GSH-Px, CAT, and GST, and the level of MDA were not substantially influenced by dietary CLA. In the liver, the specific activities of SOD, GSH-Px, and GST, and the level of MDA were also unaffected by dietary CLA at the level of either 0.75% or 1.5% compared with corn oil at the level of 1.5%. However, the broiler chickens fed the diet containing 1.5% CLA resulted in a significant increase in peroxisomal CAT activity and a marked decrease in total lipid and non-esterified fatty acids (NEFA) from liver tissues compared with those fed the diet containing 1.5% corn oil. In conclusion, ability of CLA to increase hepatic CAT activity suggest that dietary CLA may affect, at least in part, antioxidant defense system as well as lipid metabolism in the liver of broiler chickens.

Keywords

CLA;Broiler Chickens;SOD;GSH-Px;CAT;GST;MDA

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Cited by

  1. var. Jian vol.48, pp.3, 2016, https://doi.org/10.1111/jwas.12373
  2. Conjugated linoleic acid isomers strongly improve the redox status of bovine mammary epithelial cells (BME-UV1) vol.98, pp.10, 2015, https://doi.org/10.3168/jds.2015-9787

Acknowledgement

Supported by : Korea Science and Engineering Foundation (KOSEF)