Homeodomain-leucine Zipper Proteins Interact with a Plant Homologue of the Transcriptional Co-activator Multiprotein Bridging Factor 1

  • Zanetti, Maria Eugenia (Instituto de Investigaciones Biologicas, Departamento de Biologia, Universidad Nacional de Mar del Plata) ;
  • Chan, Raquel L. (Catedra de Biologia Celular y Molecular, Facultad de Bioquimica y Ciencias Biologicas, Universidad Nacional del Litoral) ;
  • Godoy, Andrea V. (Instituto de Investigaciones Biologicas, Departamento de Biologia, Universidad Nacional de Mar del Plata) ;
  • Gonzalez, Daniel H. (Catedra de Biologia Celular y Molecular, Facultad de Bioquimica y Ciencias Biologicas, Universidad Nacional del Litoral) ;
  • Casalongue, Claudia A. (Instituto de Investigaciones Biologicas, Departamento de Biologia, Universidad Nacional de Mar del Plata)
  • Published : 2004.05.31


StMBF1 (Solanum tuberosum multiprotein bridging factor 1) is a plant member of the MBF1 family of transcriptional co-activators. In an attempt to understand the role of StMBF1, we analyzed its interaction with plant transcription factors of the homeodomain-leucine zipper (Hd-Zip) family, a group of proteins with a typical leucine zipper motif adjacent to a homeodomain. StMBF1 is able to interact in vitro with the Hd-Zip protein Hahb-4 both in the presence and absence of DNA. Upon binding, StMBF1 increases the DNA binding affinity of Hahb-4, and of another plant homeodomain containing protein from the GL2/Hd-Zip IV family, HAHR-1. The biological role of interactions is discussed in this paper.


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