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Genetic Differentiation between Sheep and Goats Based on Microsatellite DNA

  • Sun, W. (Animal Science & Veterinary Medicine College, Yangzhou University) ;
  • Chang, H. (Animal Science & Veterinary Medicine College, Yangzhou University) ;
  • Ren, Z.J. (Northwest Agriculture University) ;
  • Yang, Z.P. (Animal Science & Veterinary Medicine College, Yangzhou University) ;
  • Geng, R.Q. (Animal Science & Veterinary Medicine College, Yangzhou University) ;
  • Lu, S.X. (Animal Science & Veterinary Medicine College, Yangzhou University) ;
  • Du, L. (Animal Science & Veterinary Medicine College, Yangzhou University) ;
  • Tsunoda, K. (Showa University, School of Medicine)
  • Received : 2002.11.14
  • Accepted : 2004.02.19
  • Published : 2004.05.01

Abstract

The 7 sheep microsatellite markersOarFCB48, OarAE101, MAF33, OarFCB11, MAF70, OarFCB304 and OarFCB128, which were located on chromosomes 2, 4, 6, 9, 17 and 19, were selected to PCR in Hu sheep, Tong sheep and their closely related species,the goat. They were studied with the amplifying result of 7 microsatellite sites of Hu Sheep, Tong Sheep and goats, the data of allele number and range of allele' size of amplifying were analyzed with ANOVA. The results showed that there were no significant differences (p<0.05) in microsatellite DNA sites among 3 populations. Concerning the conservation of microsatellites in closely related species, selecting microsatellite sites located on the chromosome where the Robertsonian fusion was caused between sheep and goat, may be used in research into genetic differentiation and evolutionary relationships between sheep and goats.

Keywords

Sheep;Goat;Microsatellite DNA;Genetic Differentiation

Acknowledgement

Supported by : National Natural Science Foundation of China

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