DOI QR코드

DOI QR Code

Liquid Boar Sperm Quality during Storage and In vitro Fertilization and Culture of Pig Oocytes

  • Park, C.S. (Division of Animal Science & Resources, Research Center for Transgenic Cloned Pigs Chungnam National University) ;
  • Kim, M.Y. (Division of Animal Science & Resources, Research Center for Transgenic Cloned Pigs Chungnam National University) ;
  • Yi, Y.J. (Division of Animal Science & Resources, Research Center for Transgenic Cloned Pigs Chungnam National University) ;
  • Chang, Y.J. (Division of Animal Science & Resources, Research Center for Transgenic Cloned Pigs Chungnam National University) ;
  • Lee, S.H. (College of Visual Image & Health, Kongju National University) ;
  • Lee, J.J. (Korea Thumb Vet. Co., LTD.) ;
  • Kim, M.C. (College of Veterinary Medicine, Chungnam National University) ;
  • Jin, D.I. (Division of Animal Science & Resources, Research Center for Transgenic Cloned Pigs Chungnam National University)
  • Received : 2003.12.02
  • Accepted : 2004.05.13
  • Published : 2004.10.01

Abstract

The percentages of sperm motility and normal acrosome on the liquid boar semen diluted and preserved at $4^{\circ}C$ with lactose hydrate, egg yolk and N-acetyl-D-glucosamine (LEN) diluent were significant differences according to preservation day and incubation time, respectively. The sperm motility steadily declined from 96.9% at 0.5 h incubation to 78.8% at 6 h incubation at 1 day of preservation. However, the sperm motility rapidly declined after 4 day of preservation during incubation. The normal acrosome steadily declined from 93.3% at 0.5 h incubation to 73.8% at 6 h incubation at 1 day of preservation. However, the normal acrosome rapidly declined after 3 day of preservation during incubation. The rates of sperm penetration and polyspermy were higher in 5 and $10{\times}10^6$ sperm/ml than in 0.2 and $1{\times}10^6$ sperm/ml. Mean numbers of sperm in penetrated oocyte were highest in $10{\times}10^6$ sperm/ml compared with other sperm concentrations. The rates of blastocysts from the cleaved oocytes (2-4 cell stage) were highest in $1{\times}10^6$sperm/ml compared with other sperm concentrations. In conclusion, we found out that liquid boar sperm stored at $4^{\circ}C$ could be used for in vitro fertilization of pig oocytes matured in vitro. Also, we recommend $1{\times}10^6$sperm/ml concentration for in vitro fertilization of pig oocytes.

Keywords

In vitro Fertilization;Pig Oocyte;Liquid Boar Sperm

Acknowledgement

Supported by : Korea Science & Engineering Foundation

References

  1. Johnson, L. A. 1998. Current developments in swine semen:preservation, artificial insemination and sperm sexing. In: (Ed. S. Dowe, J. Thomson and M. Varley), Proc. 15th Int. Pract. Vet. Sc., Birmingham, UK, Vol. 1. Nottingham University Press, UK, pp. 225-229.
  2. Martinez, E., J. M. Vazquez, C. Matas and J. Roca. 1996. Are washing and preincubation of boar spermatozoa really necessary to penetrate pig oocytes under in vitro conditions? Reprod. Dom. Anim. 31:317-320.
  3. Park, C. S., S. W. Han, J. S. Soh, D. I. Kim, H. K. Chung and C. G. Ryu. 1992. Study on fertilizing capacity of liquid boar semen depending on sperm concentration in 5 ml maxi-straw. Korean J. Anim. Sci. 34(2):97-100.
  4. Pursel, V. G., L. A. Johnson and G. B. Rampacek. 1972. Acrosome morphology of boar spermatozoa incubated before cold shock. J. Anim. Sci. 34:278-283.
  5. Wang, W. H., K. Niwa and K. Okuda. 1991. In vitro penetration of pig oocytes matured in culture by frozen-thawed ejaculated spermatozoa. J. Reprod. Fertil. 93:491-496.
  6. Watson, P. F. 1995. Recent developments and concepts in the cryopreservation of spermatozoa and assessment of their postthawing function. Reprod. Fertil. Dev. 7:871-891. https://doi.org/10.1071/RD9950871
  7. Yi, Y. J., Y. A. Kwon, H. J. Ko and C. S. Park. 2002. Effects of diluent component, freezing rate, thawing time and thawing temperature on acrosome morphology and motility of frozenthawed boar sperm. Asian-Aust. J. Anim. Sci. 15(11):1553-1558.
  8. Watson, P. F. 1996. Cooling of spermatozoa and fertilizing capacity. Reprod. Dom. Anim. 31:317-320. https://doi.org/10.1111/j.1439-0531.1996.tb00075.x
  9. Yi, Y. J., H. J. Ko, S. H. Lee, C. B. Yang, D. S. Son, H. K. Kim and C. S. Park. 2004. In vitro fertilization and development of pig oocytes inseminated with boar sperm by different sperm washing media after thawing of the frozen straws. Asian-Aust. J. Anim. Sci. 17(2):164-167.
  10. SAS Institute Inc. 1996. The SAS system for Windows, Release 6.12, Cary, NC.
  11. Abeydeera, L. R. and B. N. Day. 1997. Fertilization and subsequent development in vitro of pig oocytes inseminated in a modified tris-buffered medium with frozen-thawed ejaculated spermatozoa. Biol. Reprod. 57:729-734.
  12. Weitze, K. F. 1991. Long-term storage of extended boar semen. In: (L. A. Johnson and D. Rath), Proc. 2nd Int. Conf. Boar Semen Preservation, Beltsville, Maryland, USA. Reprod. Dom. Anim. (Suppl. 1), 231-253.
  13. Hamano, S. and Y. Toyoda. 1986. In vitro fertilization of pig eggs with ejaculated spermatozoa preincubated at high sperm concentration. Jpn. J. Anim. Reprod. 32:177-183.
  14. Coy, P., S. Ruiz, R. Romar, I. Campos and J. Gadea. 1999. Maturation, fertilization and complete development of porcine oocytes matured under different systems. Theriogenology 5:799-812.
  15. Suzuki, K., T. Mori and H. Shimizu. 1996. Effect of the duration of preincubation on the ability of pig spermatozoa to penetrated oocyte in vitro. Anim. Sci. Tech. Jpn. 67:24-27.
  16. Nagai, T., T. Takahashi, H. Masuda, Y. Shioya, M. Kuwayama, M. Fukushima, S. Iwasaki and A. Hamada. 1988. In vitro fertilization of frozen boar spermatozoa. J. Reprod. Fertil. 84:585-591.
  17. Nagai, T., K. Niwa and A. Iritani. 1984. Effect of sperm concentration during preincubation in a defined medium on fertilization in vitro of pig follicular oocytes. J. Reprod. Fertil. 70:271-275.
  18. Sellés, J., J. Gadea, R. Romar, C. Matás and S. Ruiz. 2003. Analysis of in vitro fertilizing capacity to evaluate the freezing procedures of boar semen and to predict the subsequent fertility. Reprod. Dom. Anim. 38:66-72.

Cited by

  1. Carbohydrate-Mediated Binding and Induction of Acrosomal Exocytosis in a Boar Sperm-Somatic Cell Adhesion Model1 vol.83, pp.4, 2010, https://doi.org/10.1095/biolreprod.110.084319