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Expression of Clostridium thermocellum Endoglucanase Gene in Lactobacillus bulgaricus and Lactobacillus plantarum and in vitro Survival Characteristics of the Transformed Lactobacilli

Lactobacillus bulgaricus와 Lactobacillus plantarum 균주에서 Clostridium thermocellum 유래 endoglucanase의 발현과 발현 유산균의 in vitro 생존 특성

  • Cho, J.S. (School of agricultural biotechnology, Seoul National University) ;
  • Kang, S.H. (School of agricultural biotechnology, Seoul National University) ;
  • Lee, H.G. (School of agricultural biotechnology, Seoul National University) ;
  • Lee, H.J. (National Livestock Research Institute, RDA) ;
  • Woo, J.H. (Laboratory of Molecular Biology, National Institute of Mental Health) ;
  • Moon, Y.S. ;
  • Yang, C.J. (Department of Animal Science and Biotechnology, Jinju National University) ;
  • Choi, Y.J. (Department of Animal Science and Technology, Sunchon National University)
  • 조재순 (서울대학교 농생명공학부) ;
  • 강승하 (서울대학교 농생명공학부) ;
  • 이홍구 (서울대학교 농생명공학부) ;
  • 이현준 (농촌진흥청 축산기술연구소) ;
  • 우정희 (미국 메릴렌드주 국립보건원) ;
  • 문양수 (진주산업대학교 동물생명과학과) ;
  • 양철주 (순천대학교 동물자원과학과) ;
  • 최윤재 (서울대학교 농생명공학부)
  • Published : 2003.08.30

Abstract

Endoglucanase A from Clostridium thermocellum which is resistant to pancreatic proteinase was selected out of numbers cellulases then were expressed in lactobacilli. Recombinant lactobacilli expression vector, pSD1, harboring the endoglucanase gene from C. thermocellum under the control of its own promoter, was constructed. Both L. bulgaricus and L. plantarum were electrotransformed with pSD1. The endoglucanase activities of 0.120 and 0.144 U/ml were found in culture media of L. bulgaricus and L. plantarum containing pSD1, respectively. In vitro survival characteristics of the transformed lactobacilli were tested. Both L. bulgaricus and L. plantarum showed a similar resistance to low pH 3. Moreover, L. plantarum was bile-salt resistant in the presence of 0.3 and 1% oxgall. L. bulgaricus and L. plantarum showed a rather homogenous resistant pattern against the tested antibiotics. Both of the strains were resistant to amikacin, gentamicin, streptomycin, kanamycin, and colistin.

Keywords

Endoglucanase activity;Clostridium thermocellum;Survival of Lactobacilli

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